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In this morphological immunohistochemical and double\u2010label confocal microscopy study we investigated the distribution of BChE in the mouse gastrointestinal tract. BChE\u2010positive cells were detected in the liver (both in hepatocytes and cholangiocytes), in the keratinised layers of the squamous epithelium of the oesophagus and forestomach, in the oxyntic mucosa of the stomach, in the mucus\u2010secreting cells of duodenal Brunner glands and the small and large intestinal mucosa. Interestingly, BChE\u2010positive cells were often detected close to gastrointestinal proliferative niches. In the oxyntic mucosa, the close proximity of ghrelin\u2010producing and BChE\u2010positive parietal cells suggests that BChE may be involved in ghrelin hydrolysation through paracrine action. 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