{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,3]],"date-time":"2026-10-03T17:06:46Z","timestamp":1791047206999,"version":"4.1.0"},"reference-count":36,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2022,12,23]],"date-time":"2022-12-23T00:00:00Z","timestamp":1671753600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Anatomy"],"published-print":{"date-parts":[[2023,5]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>The auditory tube (AT), an osteocartilaginous channel, connects the nasopharynx to the middle ear cavity. At the nasopharyngeal opening of the AT, there are dense collections of submucosal glands. In a recent article, Valstar et al. proposed these nasopharyngeal tubal glands conglomerate as salivary glands, which starkly contrasts with their previously known anatomy for being a component of the respiratory tract. This study examines the contesting views regarding the taxonomical categorization of the nasopharyngeal tubal glands.<\/jats:p><\/jats:sec><jats:sec><jats:title>Materials and Methods<\/jats:title><jats:p>The AT glands in context were examined in human cadavers grossly, and microscopically using routine and special (Hematoxylin and Eosin [H&amp;E] and Periodic acid\u2013Schiff [PAS] respectively), as well as immunohistochemical (for alpha\u2010SMA and salivary amylase) staining methods and compared with the major and minor salivary glands and the submucosal glands in the trachea. Further, a biochemical analysis was performed to detect the presence of salivary amylase in the oral and nasopharyngeal secretions of the four living human subjects, representing major salivary glands and tubal glands, respectively.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>The submucosal seromucous glands with a surface lining of respiratory epithelium were observed at the nasopharyngeal end of AT. The cells in the tubal glands showed cytoplasmic positivity for alpha\u2010SMA, which indicated the presence of the myoepithelial cells; however, this expression was significantly lower than in the seromucous submucosal glands within the trachea. Salivary alpha\u2010amylase was undetectable in the cadaveric tissue samples. Moreover, the amylase level in the nasopharyngeal swabs was negligible compared to the oral swabs.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>The anatomical location along the respiratory tract, the presence of respiratory epithelium in the overlying mucosa, their morpho\u2010functional resemblance to the seromucous glands in the trachea, and the absence of salivary amylase strongly indicate that the tubal glands are taxonomically different from the salivary glands. Given the available evidence, their existing recognition as a part of the respiratory tract and an integral component of the AT seems more appropriate.<\/jats:p><\/jats:sec>","DOI":"10.1111\/joa.13816","type":"journal-article","created":{"date-parts":[[2022,12,23]],"date-time":"2022-12-23T12:07:17Z","timestamp":1671797237000},"page":"771-780","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":12,"title":["Morpho\u2010functional characterization of the submucosal glands at the nasopharyngeal end of the auditory tube in humans"],"prefix":"10.1111","volume":"242","author":[{"given":"Chiman","family":"Kumari","sequence":"first","affiliation":[{"name":"Department of Anatomy Postgraduate Institute of Medical Education and Research (PGIMER)  Chandigarh India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Richa","family":"Gupta","sequence":"additional","affiliation":[{"name":"Department of Anatomy Postgraduate Institute of Medical Education and Research (PGIMER)  Chandigarh India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mayank","family":"Sharma","sequence":"additional","affiliation":[{"name":"Department of Anatomy Postgraduate Institute of Medical Education and Research (PGIMER)  Chandigarh India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Justin","family":"Jacob","sequence":"additional","affiliation":[{"name":"Department of Anatomy Postgraduate Institute of Medical Education and Research (PGIMER)  Chandigarh India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ravi K.","family":"Narayan","sequence":"additional","affiliation":[{"name":"Department of Anatomy, Dr. B.C. Roy Multi Specialty Medical Research Centre Indian Institute of Technology  Kharagpur India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Daisy","family":"Sahni","sequence":"additional","affiliation":[{"name":"Department of Anatomy Postgraduate Institute of Medical Education and Research (PGIMER)  Chandigarh India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1589-9568","authenticated-orcid":false,"given":"Ashutosh","family":"Kumar","sequence":"additional","affiliation":[{"name":"Department of Anatomy All India Institute of Medical Sciences (AIIMS)\u2010Patna  Patna India"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2022,12,23]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.4103\/0975\u20107406.155898"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1017\/S002221510012540X"},{"key":"e_1_2_9_4_1","volume-title":"Salivary gland disorders and diseases: diagnosis and management","author":"Bradley P.J.","year":"2011"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/J.RADONC.2020.12.004"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1002\/AR.23569"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/0046\u20108177(95)90060\u20108"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1001\/ARCHOTOL.1944.00680010374001"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1007\/S10911\u2010005\u20109586\u20104"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.2330\/JORALBIOSCI1965.27.702"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598\u2010021\u201003991\u20105"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00508431"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1002\/STEM.680"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.1159\/000358776"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/J.RADONC.2020.12.007"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.4103\/JCIS.JCIS_45_18"},{"key":"e_1_2_9_17_1","doi-asserted-by":"publisher","DOI":"10.1007\/S00268\u2010005\u20100544\u20105"},{"key":"e_1_2_9_18_1","doi-asserted-by":"publisher","DOI":"10.1007\/S00418\u2010007\u20100322\u20106"},{"key":"e_1_2_9_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/J.STEM.2018.04.007"},{"key":"e_1_2_9_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/J.ANORL.2017.03.010"},{"key":"e_1_2_9_21_1","first-page":"174","volume-title":"The Developing Human: Clinically Oriented Embryology","author":"Moore K.L.","year":"2016"},{"key":"e_1_2_9_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.radonc.2020.12.003"},{"key":"e_1_2_9_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.radonc.2020.12.016"},{"key":"e_1_2_9_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/J.RADONC.2020.12.014"},{"key":"e_1_2_9_25_1","first-page":"585","volume-title":"Histology: a text and atlas: with correlated cell and molecular biology","author":"Pawlina W.","year":"2020"},{"key":"e_1_2_9_26_1","doi-asserted-by":"publisher","DOI":"10.1007\/S11307\u2010016\u20100945\u2010X"},{"key":"e_1_2_9_27_1","doi-asserted-by":"publisher","DOI":"10.1016\/J.RADONC.2021.06.004"},{"key":"e_1_2_9_28_1","doi-asserted-by":"publisher","DOI":"10.1038\/s42003\u2010021\u201001876\u2010x"},{"key":"e_1_2_9_29_1","doi-asserted-by":"publisher","DOI":"10.1177\/00220345930720091301"},{"key":"e_1_2_9_30_1","first-page":"705","volume-title":"Gray's anatomy: the anatomical basis of clinical practice","author":"Standring S","year":"2021"},{"key":"e_1_2_9_31_1","doi-asserted-by":"publisher","DOI":"10.1186\/S13287\u2010020\u201001628\u20104\/FIGURES\/4"},{"key":"e_1_2_9_32_1","doi-asserted-by":"publisher","DOI":"10.1177\/000348948609500314"},{"key":"e_1_2_9_33_1","doi-asserted-by":"publisher","DOI":"10.1016\/J.STEM.2018.03.018"},{"key":"e_1_2_9_34_1","volume-title":"Anatomia Humana, Tomo III","author":"Testut L.A.","year":"1972"},{"key":"e_1_2_9_35_1","doi-asserted-by":"publisher","DOI":"10.3109\/00016488109133285"},{"key":"e_1_2_9_36_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.radonc.2020.09.034"},{"key":"e_1_2_9_37_1","doi-asserted-by":"publisher","DOI":"10.1016\/J.RADONC.2020.12.001"}],"container-title":["Journal of Anatomy"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/joa.13816","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/joa.13816","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/joa.13816","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,8,22]],"date-time":"2023-08-22T05:16:36Z","timestamp":1692681396000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/joa.13816"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,12,23]]},"references-count":36,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2023,5]]}},"alternative-id":["10.1111\/joa.13816"],"URL":"https:\/\/doi.org\/10.1111\/joa.13816","archive":["Portico"],"relation":{},"ISSN":["0021-8782","1469-7580"],"issn-type":[{"value":"0021-8782","type":"print"},{"value":"1469-7580","type":"electronic"}],"subject":[],"published":{"date-parts":[[2022,12,23]]},"assertion":[{"value":"2022-04-29","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2022-12-13","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2022-12-23","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}