{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,2]],"date-time":"2026-02-02T15:57:39Z","timestamp":1770047859392,"version":"3.49.0"},"reference-count":37,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2023,7,5]],"date-time":"2023-07-05T00:00:00Z","timestamp":1688515200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Anatomy"],"published-print":{"date-parts":[[2023,12]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Vagal afferent neuronal somas are in the nodose and jugular ganglia. In this study, we identified extraganglionic neurons in whole\u2010mount preparations of the vagus nerves from Phox2b\u2010Cre\u2010ZsGreen transgenic mice. These neurons are typically arranged in small clusters and monolayers along the cervical vagus nerve. Although infrequent, these neurons were sometimes observed along the thoracic and esophageal vagus. We performed RNAscope in situ hybridization and confirmed that the extraganglionic neurons detected in this transgenic mouse strain expressed vagal afferent markers (i.e., <jats:italic>Phox2b<\/jats:italic> and <jats:italic>Slc17a6<\/jats:italic>) as well as markers that identify them as potential gastrointestinal mechanoreceptors (i.e., <jats:italic>Tmc3<\/jats:italic> and <jats:italic>Glp1r<\/jats:italic>). We also identified extraganglionic neurons in the vagus nerves of wild\u2010type mice that were injected intraperitoneally with Fluoro\u2010Gold, thereby ruling out possible anatomical discrepancies specific for transgenic mice. In wild\u2010type mice, extraganglionic cells were positive for peripherin, confirming their neuronal nature. Taken together, our findings revealed a previously undiscovered population of extraganglionic neurons associated with the vagus nerve. Going forward, it is important to consider the possible existence of extraganglionic mechanoreceptors that transmit signals from the abdominal viscera in future studies related to vagal structure and function.<\/jats:p>","DOI":"10.1111\/joa.13925","type":"journal-article","created":{"date-parts":[[2023,7,5]],"date-time":"2023-07-05T12:27:26Z","timestamp":1688560046000},"page":"936-950","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Evidence of extraganglionic vagal mechanoreceptors in the mouse vagus nerve"],"prefix":"10.1111","volume":"243","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-6362-7968","authenticated-orcid":false,"given":"Luis","family":"Leon\u2010Mercado","sequence":"first","affiliation":[{"name":"Department of Internal Medicine Center for Hypothalamic Research, UT Southwestern Medical Center  Dallas Texas 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