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A sample of 30 mice (CD\u20101 mouse, <jats:italic>Mus musculus<\/jats:italic>) from five age groups (postnatal Days 2, 21, 90, 365, and 720) were used. Three\u2010dimensional mouse laryngeal thyroid cartilage reconstructions were generated from contrast\u2010enhanced micro\u2010computed tomography (CT) image stacks. Cartilage matrix composition was estimated as Hounsfield units (HU). HU were determined by overlaying 3D reconstructions as masks on micro\u2010CT image stacks and then measuring the attenuation. Cartilage shape was quantified with landmarks placed on the surface of the thyroid cartilage. Shape differences between the five age groups were analyzed using geometric morphometrics and multiparametric analysis of landmarks. The relationship between HU and shape was investigated with correlational analyses. Among five age groups, HU became higher in older animals. The shape of the thyroid cartilage changes with age throughout the entire life of a mouse. The changes in shape were not synchronized with changes in cartilage matrix composition. The thyroid cartilage of young and old <jats:italic>M. musculus<\/jats:italic> larynx showed a homogenous mineralization pattern. High\u2010resolution contrast\u2010enhanced micro\u2010CT imaging makes the mouse larynx accessible for analysis of genetic and environmental factors affecting shape and matrix composition.<\/jats:p>","DOI":"10.1111\/joa.14006","type":"journal-article","created":{"date-parts":[[2024,2,2]],"date-time":"2024-02-02T04:44:17Z","timestamp":1706849057000},"page":"739-748","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Postnatal changes in thyroid cartilage shape and cartilage matrix composition are not synchronized in <i>Mus musculus<\/i>"],"prefix":"10.1111","volume":"244","author":[{"given":"Megan","family":"Coyne","sequence":"first","affiliation":[{"name":"College of Veterinary Medicine Midwestern University  Glendale Arizona USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jolien","family":"Dellafaille","sequence":"additional","affiliation":[{"name":"Bruker Belgium  Antwerp Belgium"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6875-0017","authenticated-orcid":false,"given":"Tobias","family":"Riede","sequence":"additional","affiliation":[{"name":"College of Veterinary Medicine Midwestern University  Glendale Arizona USA"},{"name":"College of Graduate Studies, Department of Physiology Midwestern University  Glendale Arizona USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2024,2]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1177\/000348940711600810"},{"key":"e_1_2_9_3_1","unstructured":"Adams D.C. 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