{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,11,12]],"date-time":"2026-11-12T09:02:49Z","timestamp":1794474169272,"version":"4.9.1"},"reference-count":40,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2024,6,24]],"date-time":"2024-06-24T00:00:00Z","timestamp":1719187200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["AR 69047"],"award-info":[{"award-number":["AR 69047"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Anatomy"],"published-print":{"date-parts":[[2024,11]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Early diagnosis of post\u2010traumatic osteoarthritis (PTOA) is critical for designing better treatments before the degradation becomes irreversible. We utilized multimodal high\u2010resolution imaging to investigate early\u2010stage deterioration in articular cartilage and the subchondral bone plate from a sub\u2010critical impact to the knee joint, which initiates PTOA. The knee joints of 12 adult rabbits were mechanically impacted once on the femoral articular surface to initiate deterioration. At 2\u2010 and 14\u2010week post\u2010impact surgery, cartilage\u2010bone blocks were harvested from the impact region in the animals (<jats:italic>N<\/jats:italic>\u2009=\u20096 each). These blocks were assessed for deterioration using polarized light microscopy (PLM), microcomputed tomography (\u03bcCT), and biochemical analysis. Statistically significant changes were noted in the impact tissues across the calcified zone (CZ) at 14\u2009weeks post\u2010impact: the optical retardation values in the CZ of impact cartilage had a drop of 29.0% at 14\u2009weeks, while the calcium concentration in the CZ of impact cartilage also had a significant drop at 14\u2009weeks. A significant reduction of 6.3% in bone mineral density (BMD) was noted in the subchondral bone plate of the impact samples at 14\u2009weeks. At 2\u2009weeks post\u2010impact, only minor, non\u2010significant changes were measured. Furthermore, the impact knees after 14\u2009weeks had greater structural changes compared with the 2\u2010week impact knees, indicating progressive degradation over time. The findings of this study facilitated a connection between mineralization alterations and the early deterioration of knee cartilage after a mechanical injury. In a broader context, these findings can be beneficial in improving clinical strategies to manage joint injuries.<\/jats:p>","DOI":"10.1111\/joa.14102","type":"journal-article","created":{"date-parts":[[2024,6,25]],"date-time":"2024-06-25T06:13:47Z","timestamp":1719296027000},"page":"740-750","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Assessment of post\u2010trauma microstructural alterations in the rabbit knee cartilage and subchondral bone"],"prefix":"10.1111","volume":"245","author":[{"ORCID":"https:\/\/orcid.org\/0009-0008-4834-2663","authenticated-orcid":false,"given":"Amanveer","family":"Singh","sequence":"first","affiliation":[{"name":"Department of Physics and Center for Biomedical Research Oakland University  Rochester Michigan 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