{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,3,12]],"date-time":"2025-03-12T04:40:00Z","timestamp":1741754400307,"version":"3.38.0"},"reference-count":31,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2024,9,5]],"date-time":"2024-09-05T00:00:00Z","timestamp":1725494400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Anatomy"],"published-print":{"date-parts":[[2025,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Counting growth layers in dentine and\/or secondary cementum is widely used for age determination in wild mammals but the underlying seasonal changes in the structure and degree of mineralisation of dental tissue have not been well characterised. We embedded first (m1) and second (m2) mandibular permanent molar teeth from a 12\u2010year\u2010old female Svalbard reindeer (<jats:italic>Rangifer tarandus platyrhynchus<\/jats:italic>) in PolyMethylMethAcrylate (PMMA), prepared cut and polished surfaces coated with evaporated carbon and used 20\u2009kV back\u2010scattered electron imaging in a scanning electron microscope (BSE\u2010SEM) to study aspects of dental tissue structure which depend on the degree of mineralisation at the micron and sub\u2010micron scale. BSE\u2010SEM revealed differences between the mineral content of growth layers (annulations) in the secondary cementum and the primary and secondary dentine, the latter, incidentally, still forming at death in m1. Wide bands of less well mineralised tissue formed in the cementum during active appositional phases. Thin, denser bands formed by maturation\u2010mineralisation of existing tissue when growth slowed in winter. This maturation mimics the processes seen in lamellar bone and articular cartilage. Counter to previous suggestions, there was evidence of substantial resorption and repair of the secondary cementum and of formation of dentine throughout life. Secondary dentine is layered by mineral content like cementum. In the crown, this was mainly tubular dentine with well\u2010marked interglobular dentine layers. In the lower pulp chamber and root, it was largely without tubules. Substantial non\u2010mineralised spaces found at the cement\u2010dentine junction in the root apical regions in m2 represent inclusions of the Hertwig's Epithelial Root Sheath (HERS) or the Epithelial Rests of Malassez (ERM) between the two tissues, a phenomenon which has previously only been identified in Muridae. The anatomical changes which result in the formation of the incremental lines (annulations) in dental tissues of reindeer, identified here for the first time at the micrometre level, are likely to be common across most if not all long\u2010lived species of mammals living in seasonal environments.<\/jats:p>","DOI":"10.1111\/joa.14135","type":"journal-article","created":{"date-parts":[[2024,9,5]],"date-time":"2024-09-05T10:05:08Z","timestamp":1725530708000},"page":"160-167","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Microanatomy of incremental growth lines in dental tissues in reindeer <i>Rangifer tarandus<\/i>"],"prefix":"10.1111","volume":"246","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-9871-5498","authenticated-orcid":false,"given":"Alan","family":"Boyde","sequence":"first","affiliation":[{"name":"Dental Physical Sciences Unit, Institute of Dentistry Queen Mary University of London  London UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7074-9135","authenticated-orcid":false,"given":"Nicholas J. C.","family":"Tyler","sequence":"additional","affiliation":[{"name":"Centre for Saami Studies UiT the Arctic University of Norway  Troms\u00f8 Norway"},{"name":"Department of Agricultural Sciences Lincoln University  Christchurch New Zealand"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2024,9,5]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-051X.1990.tb01690.x"},{"key":"e_1_2_10_3_1","first-page":"65","volume-title":"Growth of odontocetes and sirenians: problems in age determination: proceedings of the international conference on determining age of odontocete cetaceans [and sirenians]","author":"Boyde A.","year":"1980"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.1007\/978\u20101\u20104939\u20108997\u20103_31"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00223\u2010021\u201000866\u20109"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.1201\/9781351189590-13"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00315817"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598\u2010020\u201062177\u20107"},{"key":"e_1_2_10_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/s8756\u20103282(01)00686\u2010x"},{"key":"e_1_2_10_10_1","first-page":"1","article-title":"Review of the formation of incremental lines in tooth cementum of terrestrial mammals","volume":"11","author":"Grue H.","year":"1979","journal-title":"Danish Review of Game Biology"},{"key":"e_1_2_10_11_1","first-page":"81","volume-title":"Dentin and Dentinogenesis","author":"Jones S.J.","year":"1984"},{"key":"e_1_2_10_12_1","unstructured":"Klevezal G.A.&Kleinenberg S.E.(1967)Opredelenie vozrasta mlekopitayushchikh po sloistym strukturam zubov i kosti. IMZh AN SSSR. Institut Morfologii Zhivotnykh. Im. A.N. Severtsova. 144 p. [English translation:Age determination of mammals from annual layers in teeth and bones. Jerusalem: Israel program for scientific translations.]."},{"key":"e_1_2_10_13_1","doi-asserted-by":"publisher","DOI":"10.1038\/169972b0"},{"key":"e_1_2_10_14_1","first-page":"11","article-title":"A new method of age determination in mammals with special reference to the elephant seal","volume":"2","author":"Laws R.M.","year":"1953","journal-title":"Falkland Islands Dependencies Survey, Scientific Report"},{"key":"e_1_2_10_15_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1469-7998.1979.tb03431.x"},{"key":"e_1_2_10_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/s0022\u20105320(70)90119\u2010x"},{"key":"e_1_2_10_17_1","doi-asserted-by":"publisher","DOI":"10.1006\/jasc.1994.1052"},{"issue":"2","key":"e_1_2_10_18_1","first-page":"430","article-title":"Age determination of deer by annular structure of dental cementum","volume":"30","author":"Low W.A.","year":"1963","journal-title":"Journal of Wildlife Management"},{"key":"e_1_2_10_19_1","doi-asserted-by":"publisher","DOI":"10.1038\/186092a0"},{"key":"e_1_2_10_20_1","unstructured":"Miller F.L.(1974)Biology of the Kaminuriak population of barren\u2010ground caribou. Part 2: dentition as an indicator of age and sex: composition and socialisation of the population. Canadian Wildlife Service Report Series Nr.31. Ottawa."},{"key":"e_1_2_10_21_1","doi-asserted-by":"publisher","DOI":"10.1038\/198350a0"},{"key":"e_1_2_10_22_1","doi-asserted-by":"publisher","DOI":"10.2307\/2912"},{"volume-title":"Age determination of toothed whales and sirenians. 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