{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,16]],"date-time":"2026-04-16T15:30:57Z","timestamp":1776353457179,"version":"3.51.2"},"reference-count":34,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2024,10,5]],"date-time":"2024-10-05T00:00:00Z","timestamp":1728086400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Anatomy"],"published-print":{"date-parts":[[2025,2]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The sentinel lymph node (SN) concept has a significant impact on cancer surgery. We aimed to examine which morphology of dendritic cells (DCs) and macrophages corresponds to \u201cpreconditioning\u201d of the SN against cancer. Although macrophages are generally able to tolerate cancer metastasis, the CD169\u2010positive subtype is believed to be a limited exception. Immunohistochemical and morphometric analyses were performed to examine DC\u2010SIGN\u2010, CD68\u2010, and CD169\u2010positive cells in SNs and non\u2010SNs of 23 patients with gastric cancer with or without nodal metastasis. All patients survived for &gt;5\u2009years without recurrence. DCs were present in the subcapsular, paracortical, and medullary sinuses, the endothelia of which expressed DC\u2010SIGN and smooth muscle actin (SMA). In the non\u2010SNs of patients without metastasis, subcapsular DCs occupied a larger area than SNs, and this difference was statistically significant. Conversely, subcapsular DCs were likely to have migrated to the paracortical area of the SNs. DC clusters often overlapped with macrophage clusters; however, histiocytosis\u2010like clusters of CD169\u2010negative macrophages showed a smaller overlap. We found a significantly larger overlap between DC\u2010SIGN and CD169\u2010positive clusters in SNs than in non\u2010SNs; the larger overlap seemed to correspond to a higher cross\u2010presentation of cancer antigens between these cell populations. DC\u2010SIGN\u2013CD169\u2010double positive cells might exist within this overlap. SNs in gastric cancers are usually preconditioned as a frontier of cancer immunity, but they may sometimes be suppressed earlier than non\u2010SNs. DC\u2010SIGN\u2010 and CD169\u2010positive cells appeared to decrease owing to a long lag time from the primary lesion occurrence and a short distance from the metastasis.<\/jats:p>","DOI":"10.1111\/joa.14147","type":"journal-article","created":{"date-parts":[[2024,10,5]],"date-time":"2024-10-05T07:47:53Z","timestamp":1728114473000},"page":"272-287","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Difference between sentinel and non\u2010sentinel lymph nodes in the distribution of dendritic cells and macrophages: An immunohistochemical and morphometric study using gastric regional nodes obtained in sentinel node navigation surgery for early gastric cancer"],"prefix":"10.1111","volume":"246","author":[{"ORCID":"https:\/\/orcid.org\/0009-0003-3356-4224","authenticated-orcid":false,"given":"Tomohiro","family":"Sonoda","sequence":"first","affiliation":[{"name":"Department of Digestive Surgery, Graduate School of Medical 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