{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,11]],"date-time":"2026-06-11T12:19:02Z","timestamp":1781180342792,"version":"3.54.1"},"reference-count":67,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2024,12,26]],"date-time":"2024-12-26T00:00:00Z","timestamp":1735171200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"DOI":"10.13039\/100000001","name":"National Science Foundation","doi-asserted-by":"publisher","award":["1149453"],"award-info":[{"award-number":["1149453"]}],"id":[{"id":"10.13039\/100000001","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000001","name":"National Science Foundation","doi-asserted-by":"publisher","award":["1827647"],"award-info":[{"award-number":["1827647"]}],"id":[{"id":"10.13039\/100000001","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100008227","name":"Achievement Rewards for College Scientists Foundation","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100008227","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100009988","name":"Society for Developmental Biology","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100009988","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000057","name":"National Institute of General Medical Sciences","doi-asserted-by":"publisher","award":["T32GM007103"],"award-info":[{"award-number":["T32GM007103"]}],"id":[{"id":"10.13039\/100000057","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Anatomy"],"published-print":{"date-parts":[[2025,6]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The fovea, a pit in the retina, is crucial for high\u2010acuity vision in humans and is found in the eyes of other vertebrates, including certain primates, birds, lizards, and fish. Despite its importance for vision, our understanding of the mechanisms involved in fovea development remains limited. Widely used ocular research models lack a foveated retina, and studies on fovea development are mostly limited to histological and molecular studies in primates. As a first step toward elucidating fovea development in nonprimate vertebrates, we present a detailed histological atlas of retina and fovea development in the bifoveated <jats:italic>Anolis sagrei<\/jats:italic> lizard, a novel reptile model for fovea research. We test the hypothesis that retinal remodeling, leading to fovea formation and photoreceptor cell packing, is related to asymmetric changes in eye shape. Our findings show that anole retina development follows the typical spatiotemporal patterning observed in most vertebrates: retinal neurogenesis starts in the central retina, progresses through the temporal retina, and finishes in the nasal retina. However, the areas destined to become the central or temporal fovea differentiate earlier than the rest of the retina. We observe dynamic changes in retinal thickness during ocular elongation and retraction\u2014thinning during elongation and thickening during retraction. Additionally, a transient localized thickening of the ganglion cell layer occurs in the temporal fovea region just before pit formation. Our data indicate that anole retina development is similar to that of humans, including the onset and progression of retinal neurogenesis, followed by changes in ocular shape and retinal remodeling leading to pit formation. We propose that anoles are an excellent model system for fovea development research.<\/jats:p>","DOI":"10.1111\/joa.14193","type":"journal-article","created":{"date-parts":[[2024,12,27]],"date-time":"2024-12-27T05:51:01Z","timestamp":1735278661000},"page":"1019-1033","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Histological analysis of retinal development and remodeling in the brown anole lizard (<i>Anolis sagrei<\/i>)"],"prefix":"10.1111","volume":"246","author":[{"given":"Ashley M.","family":"Rasys","sequence":"first","affiliation":[{"name":"Department of Cellular Biology The University of Georgia  Athens Georgia USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shana H.","family":"Pau","sequence":"additional","affiliation":[{"name":"Department of Genetics The University of Georgia  Athens Georgia USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Katherine E.","family":"Irwin","sequence":"additional","affiliation":[{"name":"Department of Cellular Biology The University of Georgia  Athens Georgia USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sherry","family":"Luo","sequence":"additional","affiliation":[{"name":"Department of Genetics The University of Georgia  Athens Georgia USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hannah Q.","family":"Kim","sequence":"additional","affiliation":[{"name":"Department of Cellular Biology The University of Georgia  Athens Georgia USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M. Austin","family":"Wahle","sequence":"additional","affiliation":[{"name":"Department of Genetics The University of Georgia  Athens Georgia USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Douglas B.","family":"Menke","sequence":"additional","affiliation":[{"name":"Department of Genetics The University of Georgia  Athens Georgia USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7503-0528","authenticated-orcid":false,"given":"James D.","family":"Lauderdale","sequence":"additional","affiliation":[{"name":"Department of Cellular Biology The University of Georgia  Athens Georgia USA"},{"name":"Neuroscience Division of the Biomedical and Translational Sciences Institute The University of Georgia  Athens Georgia USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2024,12,26]]},"reference":[{"key":"e_1_2_10_2_1","volume-title":"AVMA guidelines for the euthanasia of animals: 2020 Edition","author":"Association AVM","year":"2020"},{"key":"e_1_2_10_3_1","volume-title":"Atlas zur Entwicklungsgeschichte des menschlichen Auges","author":"Bach L.","year":"1911"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00685239"},{"key":"e_1_2_10_5_1","volume-title":"Embryology of the human eye","author":"Barber A.N.","year":"1955"},{"key":"e_1_2_10_6_1","first-page":"1","volume-title":"Embryology of the eye and its adnexa","author":"Barishak Y.R.","year":"1992"},{"key":"e_1_2_10_7_1","first-page":"169","article-title":"An investigation into the role of ganglion cells in the regulation of division and death of other retinal cells","volume":"430","author":"Beazley L.D.","year":"1987","journal-title":"Retinal Development"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.1111\/ahe.12432"},{"key":"e_1_2_10_9_1","first-page":"369","article-title":"The foveal photoreceptor mosaic in the pipefish, Corythoichthyes paxtoni (Syngnathidae, Teleostei)","volume":"14","author":"Collin S.P.","year":"1999","journal-title":"Histology and Histopathology"},{"key":"e_1_2_10_10_1","first-page":"28","article-title":"Use of tricaine methanesulfonate (MS222) for euthanasia of reptiles","volume":"48","author":"Conroy C.J.","year":"2009","journal-title":"Journal of the American Association for Laboratory Animal Science: JAALAS"},{"key":"e_1_2_10_11_1","doi-asserted-by":"publisher","DOI":"10.1002\/aja.1000960106"},{"key":"e_1_2_10_12_1","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.12-06-02381.1992"},{"key":"e_1_2_10_13_1","doi-asserted-by":"publisher","DOI":"10.1159\/000121639"},{"key":"e_1_2_10_14_1","doi-asserted-by":"publisher","DOI":"10.1159\/000124142"},{"key":"e_1_2_10_15_1","doi-asserted-by":"publisher","DOI":"10.3389\/famrs.2024.1426675"},{"key":"e_1_2_10_16_1","doi-asserted-by":"publisher","DOI":"10.1038\/eye.1992.29"},{"key":"e_1_2_10_17_1","doi-asserted-by":"publisher","DOI":"10.1007\/3-540-26977-0_1"},{"key":"e_1_2_10_18_1","first-page":"29","article-title":"Development of retinal layers in prenatal human retina","volume":"166","author":"Hendrickson A.","year":"2015","journal-title":"American Journal of Ophthalmology"},{"key":"e_1_2_10_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0166-4328(05)80191-3"},{"key":"e_1_2_10_20_1","first-page":"746","article-title":"The histogenesis of the fovea in the macaque monkey","volume":"15","author":"Hendrickson A.","year":"1976","journal-title":"Investigative Ophthalmology & Visual Science"},{"key":"e_1_2_10_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ajo.2012.05.007"},{"key":"e_1_2_10_22_1","doi-asserted-by":"publisher","DOI":"10.1017\/CBO9780511541629.009"},{"key":"e_1_2_10_23_1","doi-asserted-by":"publisher","DOI":"10.1002\/cne.24170"},{"key":"e_1_2_10_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0161-6420(84)34247-6"},{"key":"e_1_2_10_25_1","first-page":"472","article-title":"Early development of the human retina","volume":"7","author":"Hollenberg M.J.","year":"1972","journal-title":"Canadian Journal of Ophthalmology"},{"key":"e_1_2_10_26_1","doi-asserted-by":"publisher","DOI":"10.1002\/aja.1001370402"},{"key":"e_1_2_10_27_1","doi-asserted-by":"publisher","DOI":"10.1098\/rstl.1866.0011"},{"key":"e_1_2_10_28_1","doi-asserted-by":"publisher","DOI":"10.1016\/0012-1606(74)90256-5"},{"key":"e_1_2_10_29_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF02000705"},{"key":"e_1_2_10_30_1","doi-asserted-by":"publisher","DOI":"10.1002\/cne.903090107"},{"key":"e_1_2_10_31_1","doi-asserted-by":"publisher","DOI":"10.1017\/S0952523800000067"},{"key":"e_1_2_10_32_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1442-9071.1992.tb00740.x"},{"key":"e_1_2_10_33_1","doi-asserted-by":"publisher","DOI":"10.1016\/0042-6989(80)90092-9"},{"key":"e_1_2_10_34_1","volume-title":"The development of the human eye","author":"Mann I.C.","year":"1928"},{"key":"e_1_2_10_35_1","volume-title":"Raptor vision","author":"Mitkus M.","year":"2018"},{"key":"e_1_2_10_36_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10695-015-0082-5"},{"key":"e_1_2_10_37_1","doi-asserted-by":"publisher","DOI":"10.1111\/jfb.13668"},{"key":"e_1_2_10_38_1","doi-asserted-by":"publisher","DOI":"10.1159\/000141425"},{"key":"e_1_2_10_39_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2019.01.004"},{"key":"e_1_2_10_40_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1460-9568.1991.tb00843.x"},{"key":"e_1_2_10_41_1","doi-asserted-by":"publisher","DOI":"10.1002\/cne.902330403"},{"key":"e_1_2_10_42_1","doi-asserted-by":"publisher","DOI":"10.1177\/0023677219862841"},{"key":"e_1_2_10_43_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.celrep.2019.07.089"},{"key":"e_1_2_10_44_1","doi-asserted-by":"publisher","DOI":"10.1002\/dvdy.348"},{"key":"e_1_2_10_45_1","doi-asserted-by":"crossref","unstructured":"Rasys A.M. Pau S.H. Irwin K.E. Luo S. Menke D.B.&Lauderdale J.D.(2021)Anterior eye development in the brown anole Anolis sagrei.bioRxiv 1\u201321.https:\/\/doi.org\/10.1101\/2021.02.15.429783","DOI":"10.1101\/2021.02.15.429783"},{"key":"e_1_2_10_46_1","doi-asserted-by":"publisher","DOI":"10.1002\/bies.202300054"},{"key":"e_1_2_10_47_1","doi-asserted-by":"publisher","DOI":"10.1002\/aja.1001540206"},{"key":"e_1_2_10_48_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0042-6989(01)00093-1"},{"key":"e_1_2_10_49_1","first-page":"58","article-title":"Laboratory protocols for husbandry and embryo collection of Anolis lizards","volume":"39","author":"Sanger T.J.","year":"2008","journal-title":"Herpetological Review"},{"key":"e_1_2_10_50_1","doi-asserted-by":"publisher","DOI":"10.1002\/jmor.10563"},{"key":"e_1_2_10_51_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.exer.2018.05.012"},{"key":"e_1_2_10_52_1","doi-asserted-by":"publisher","DOI":"10.1002\/jmor.1050130304"},{"key":"e_1_2_10_53_1","doi-asserted-by":"publisher","DOI":"10.1201\/9781420041606"},{"key":"e_1_2_10_54_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00683263"},{"key":"e_1_2_10_55_1","doi-asserted-by":"publisher","DOI":"10.1017\/S0952523804041057"},{"key":"e_1_2_10_56_1","doi-asserted-by":"publisher","DOI":"10.1017\/S0952523804215115"},{"key":"e_1_2_10_57_1","doi-asserted-by":"publisher","DOI":"10.1017\/S095252380522206X"},{"key":"e_1_2_10_58_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-020-78452-6"},{"key":"e_1_2_10_59_1","first-page":"1","volume-title":"Biology of the reptilia, volume 2. Morphology B","author":"Underwood G.","year":"1970"},{"key":"e_1_2_10_60_1","doi-asserted-by":"publisher","DOI":"10.1002\/cne.902910205"},{"key":"e_1_2_10_61_1","first-page":"1","article-title":"Morphological research on the \u201cscleral protuberance\u201d","volume":"163","author":"Van L.","year":"1961","journal-title":"Albrecht von Graefe's Archiv f\u00fcr Ophthalmologie"},{"key":"e_1_2_10_62_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.exer.2019.05.001"},{"key":"e_1_2_10_63_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF02721221"},{"key":"e_1_2_10_64_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.exer.2023.109611"},{"key":"e_1_2_10_65_1","doi-asserted-by":"publisher","DOI":"10.5962\/bhl.title.7369"},{"key":"e_1_2_10_66_1","doi-asserted-by":"publisher","DOI":"10.1086\/278664"},{"key":"e_1_2_10_67_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-020-66092-9"},{"key":"e_1_2_10_68_1","doi-asserted-by":"publisher","DOI":"10.1016\/0042-6989(86)90143-4"}],"container-title":["Journal of Anatomy"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/joa.14193","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,5,15]],"date-time":"2025-05-15T12:24:12Z","timestamp":1747311852000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/joa.14193"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,12,26]]},"references-count":67,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2025,6]]}},"alternative-id":["10.1111\/joa.14193"],"URL":"https:\/\/doi.org\/10.1111\/joa.14193","archive":["Portico"],"relation":{},"ISSN":["0021-8782","1469-7580"],"issn-type":[{"value":"0021-8782","type":"print"},{"value":"1469-7580","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,12,26]]},"assertion":[{"value":"2024-09-08","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-11-22","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-12-26","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}