{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,8,2]],"date-time":"2025-08-02T14:38:10Z","timestamp":1754145490085,"version":"3.41.2"},"reference-count":25,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2025,3,10]],"date-time":"2025-03-10T00:00:00Z","timestamp":1741564800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Anatomy"],"published-print":{"date-parts":[[2025,8]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The vasa vasorum (VV) have gathered considerable interest over the last decade due to its role in vascular wall biology and pathology; however, while the coronary VV are relatively well studied, the anatomy of peripheral VV, such as those of the aorta, remains poorly described, hampering knowledge of their role in peripheral vascular diseases. Through careful retrieval of the porcine thoracic aorta and successful microthrombi removal, the thoracic aorta was perfused with BriteVu\u2122 followed by micro\u2010computed tomography (micro\u2010CT) scanning to image the VV and obtain a 3D reconstruction. We used commercially available software and its thresholding algorithms. The arterial segments were further dehydrated and stained in an iodine solution to delineate the tunica adventitia and tunica media on imaging. A meticulous technique of harvest, clearing and perfusion of the porcine aorta was developed. BriteVu\u2122 perfusion enabled clear visualization of the VV structure, branching pattern and distribution with a voxel size of 9\u2009\u03bcm. Additional iodine staining differentiated VV residing in the tunica media versus the tunica adventitia of the vessel. The technique we have developed allows detailed knowledge of peripheral VV anatomy and distribution to be obtained where previous information was scarce. Detailed anatomical study of the VV and its relation to branches will further our understanding of its relationship to vascular disease processes.<\/jats:p>","DOI":"10.1111\/joa.14238","type":"journal-article","created":{"date-parts":[[2025,3,12]],"date-time":"2025-03-12T01:21:59Z","timestamp":1741742519000},"page":"385-392","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Enhanced imaging of porcine thoracic aorta vasa vasorum with contrast micro\u2010computed tomography"],"prefix":"10.1111","volume":"247","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-8663-4714","authenticated-orcid":false,"given":"Bichen","family":"Zhao","sequence":"first","affiliation":[{"name":"Targeted Drug Delivery, Imaging and Therapy Laboratory, Harry Perkins Institute of Medical Research, QEII Medical Centre and Centre for Medical Research The University of Western Australia  Perth Western Australia Australia"},{"name":"Curtin Medical School Curtin University  Perth Western Australia Australia"},{"name":"Department of Vascular and Endovascular Surgery Sir Charles Gairdner Hospital  Nedlands Western Australia Australia"},{"name":"Faculty of Health Sciences University of Western Australia  Perth Western Australia Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hanane","family":"Belhoul\u2010Fakir","sequence":"additional","affiliation":[{"name":"Targeted Drug Delivery, Imaging and Therapy Laboratory, Harry Perkins Institute of Medical Research, QEII Medical Centre and Centre for Medical Research The University of Western Australia  Perth Western Australia Australia"},{"name":"Curtin Medical School Curtin University  Perth Western Australia Australia"},{"name":"Faculty of Health Sciences University of Western Australia  Perth Western Australia Australia"},{"name":"Heart and Vascular Research Institute Harry Perkins Institute for Medical Research  Perth Western Australia 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