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Immunohistochemical and morphometric analyses were performed to examine DC\u2010SIGN\u2010, CD68\u2010, and CD169\u2010positive cells in the subcarinal node (proximal) and paratracheal nodes (distal nodes) from 16 patients with lower\u2010lobe lung cancer without metastasis (adenocarcinoma, 11; squamous, 5). Nodes at the same sites from 10 patients with upper\u2010lobe cancer were used as controls. In all nodes, the medullary sinus was filled with CD68\u2010positive and CD169\u2010negative macrophages, most of which showed anthracosis. The proximal node carried a significantly smaller overlap between clusters of DC\u2010SIGN\u2010positive cells and CD169\u2010positive cells relative to the distal node in lower\u2010lobe cancer patients (<jats:italic>p<\/jats:italic>\u2009=\u20090.015). Irrespective of the cancer pathology, the tumor size was significantly correlated with the longer subcapsular clusters containing either DC\u2010SIGN\u2010positive cells or CD169\u2010positive cells (<jats:italic>p<\/jats:italic>\u2009=\u20090.003, 0.043). A significantly small overlap between these clusters as well as the missing paracortical sinuses was evident in the negative control node outside the lymph flow (<jats:italic>p<\/jats:italic>\u2009=\u20090.006). Since DC\u2010SIGN\u2010positive cells and CD169\u2010positive cells, especially composite cells in the overlapped cluster, are likely to be derived from monocytes, larger tumors appeared to accelerate the migration into the subcapsular sinus. In contrast to the suggested active status of the distal node, the proximal node appeared to have already been suppressed. This downregulation reached the level in the negative control node.<\/jats:p>","DOI":"10.1111\/joa.14251","type":"journal-article","created":{"date-parts":[[2025,3,30]],"date-time":"2025-03-30T14:56:43Z","timestamp":1743346603000},"page":"393-407","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Topohistology of dendritic cells and macrophages in the distal and proximal nodes along the lymph flow from the lung"],"prefix":"10.1111","volume":"247","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-1769-5382","authenticated-orcid":false,"given":"Masaya","family":"Aoki","sequence":"first","affiliation":[{"name":"Department of General Thoracic Surgery, Graduate School of Medical and Dental Sciences Kagoshima University  Kagoshima Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Go","family":"Kamimura","sequence":"additional","affiliation":[{"name":"Department of General Thoracic Surgery, Graduate 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