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In mammals and crocodiles, the teeth are cushioned inside bony sockets by periodontal ligaments, whereas most squamate reptiles have teeth firmly attached to the jawbone. Here, we analyzed the development of the attachment tissue in the veiled chameleon, a species with firm acrodont tooth attachment, to reveal the cellular processes establishing ankylosis and to determine the cell types contributing to the attachment. The tooth\u2010bearing bones formed pedicles with edges fusing to the dentine via an attachment tissue produced by morphologically distinct cells exhibiting both osteoblastic and odontoblastic features. These cells were RUNX2\u2010positive, suggesting their potential to differentiate into hard\u2010tissue\u2010producing cells. However, in contrast to the osteoblasts of the bony pedicles, tooth\u2013bone interface (TBI) cells expressed elevated levels of Na\n                    <jats:sup>+<\/jats:sup>\n                    \u2010\/K\n                    <jats:sup>+<\/jats:sup>\n                    \u2010ATPase and thus resembled odontoblasts. TBI cells were visible only temporarily, and after tooth\u2013bone fusion they were removed by apoptosis and phagocytosis. Dynamic deposition of the hard matrix continued on both sides of the TBI and during the posthatching stages through the participation of osteoblasts. Overall, our findings demonstrate both odontoblast\u2010 and osteoblast\u2010like characteristics of cells producing the attachment tissue at the TBI during development in chameleons, highlighting the existence of a transient intermediate cell population, which we call ankyloblasts.\n                  <\/jats:p>","DOI":"10.1111\/joa.70059","type":"journal-article","created":{"date-parts":[[2025,11,20]],"date-time":"2025-11-20T07:43:22Z","timestamp":1763624602000},"page":"251-268","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Tooth\u2013bone attachment tissue is produced by cells with a mixture of odontoblastic and osteoblastic features in reptiles"],"prefix":"10.1111","volume":"248","author":[{"given":"M.","family":"\u0160ulcov\u00e1","sequence":"first","affiliation":[{"name":"Department of Experimental Biology, Faculty of Science Masaryk University  Brno Czech Republic"},{"name":"Laboratory of Molecular Morphogenesis, Institute of Animal Physiology and Genetics v.v.i., Czech Academy of Sciences  Brno Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J.","family":"Dumkov\u00e1","sequence":"additional","affiliation":[{"name":"Department of Histology and Embryology, Faculty of Medicine Masaryk University  Brno Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"B.","family":"Hute\u010dkov\u00e1","sequence":"additional","affiliation":[{"name":"Department of Experimental Biology, Faculty of Science Masaryk University  Brno Czech Republic"},{"name":"Laboratory of Molecular Morphogenesis, Institute of Animal Physiology and Genetics v.v.i., Czech Academy of Sciences  Brno Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M.","family":"Kavkov\u00e1","sequence":"additional","affiliation":[{"name":"Central European Institute of Technology Brno University of Technology  Brno Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"V.","family":"Parobkov\u00e1","sequence":"additional","affiliation":[{"name":"Central European Institute of Technology Brno University of Technology  Brno Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"O.","family":"Zahradn\u00ed\u010dek","sequence":"additional","affiliation":[{"name":"Department of Radiation Dosimetry, Nuclear Physics Institute Czech Academy of Sciences  Prague Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J.","family":"K\u0159iv\u00e1nek","sequence":"additional","affiliation":[{"name":"Department of Histology and Embryology, Faculty of Medicine Masaryk University  Brno Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"I.","family":"Adameyko","sequence":"additional","affiliation":[{"name":"Department of Neuroimmunology, Center for Brain Research Medical University of Vienna  Vienna Austria"},{"name":"Department of Physiology and Pharmacology Karolinska Institutet  Stockholm Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J.","family":"Kaiser","sequence":"additional","affiliation":[{"name":"Central European Institute of Technology Brno University of Technology  Brno Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"T.","family":"Zikmund","sequence":"additional","affiliation":[{"name":"Central European Institute of Technology Brno University of Technology  Brno Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8871-6094","authenticated-orcid":false,"given":"A. 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