{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,9]],"date-time":"2026-10-09T01:26:58Z","timestamp":1791509218356,"version":"4.3.4"},"reference-count":30,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2015,7,14]],"date-time":"2015-07-14T00:00:00Z","timestamp":1436832000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J of Cosmetic Dermatology"],"published-print":{"date-parts":[[2015,9]]},"abstract":"<jats:title>Summary<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>The<jats:italic>chromosomal passenger complex<\/jats:italic>(<jats:styled-content style=\"fixed-case\">CPC<\/jats:styled-content>) is an assembly made of four interacting proteins: survivin, borealin,<jats:styled-content style=\"fixed-case\">INCENP<\/jats:styled-content>, and aurora kinase B.<jats:styled-content style=\"fixed-case\">CPC<\/jats:styled-content>is the key regulatory complex responsible for the correct development of cellular mitosis, accompanying each step of the chromosomal segregation. This control of mitosis is particularly important in undifferentiated cells that must renew themselves and also further differentiate and specialize. The epidermis is a self\u2010renewing tissue that needs to continuously generate new cells through proliferation and differentiation of progenitor cells. Both the mitosis supervision by the<jats:styled-content style=\"fixed-case\">CPC<\/jats:styled-content>and a correct extracellular environment are physiologically required for the homeostasis of the adult keratinocyte stem cells (<jats:styled-content style=\"fixed-case\">KSC<\/jats:styled-content>s) of the epidermis.<jats:styled-content style=\"fixed-case\">KSC<\/jats:styled-content>s are mainly found in the basal layer of the epidermis and are responsible for the replenishment and maintenance of the tissue, by compensating for the loss of terminally differentiated cells called corneocytes, especially during aging.<\/jats:p><\/jats:sec><jats:sec><jats:title>Aim<\/jats:title><jats:p>The aim of our study was to investigate the implication of survivin in epidermal renewal and the relationships between survivin expression and<jats:styled-content style=\"fixed-case\">UVB<\/jats:styled-content>\u2010induced<jats:styled-content style=\"fixed-case\">DNA<\/jats:styled-content>damage levels in cultured human keratinocytes and in skin biopsies. In parallel, the effects of a treatment by compound<jats:styled-content style=\"fixed-case\">IV<\/jats:styled-content>08.009 were studied.<\/jats:p><\/jats:sec><jats:sec><jats:title>Material and methods<\/jats:title><jats:p>Cultured human keratinocytes and skin biopsies were used in this study.<jats:styled-content style=\"fixed-case\">KSC<\/jats:styled-content>s\u2010enriched fractions of keratinocytes were isolated from total keratinocytes by differential attachment to a type<jats:styled-content style=\"fixed-case\">IV<\/jats:styled-content>collagen matrix. Survivin expression levels were assessed by immunoblotting in cultured keratinocytes, and \u03b16\u2010integrin, \u03b21\u2010integrin, keratin 15, and survivin were observed after immunodetection in skin biopsies cross sections. Comet assay, immunodetection of<jats:styled-content style=\"fixed-case\">CPD<\/jats:styled-content>s and of cleaved\u2010caspase 3, and electron microscopy were used to characterize<jats:styled-content style=\"fixed-case\">UVB<\/jats:styled-content>\u2010induced<jats:styled-content style=\"fixed-case\">DNA<\/jats:styled-content>damage.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>We demonstrated the ability of compound<jats:styled-content style=\"fixed-case\">IV<\/jats:styled-content>08.009 to efficiently protect<jats:italic>ex\u00a0vivo<\/jats:italic>skin against basal<jats:styled-content style=\"fixed-case\">UVB<\/jats:styled-content>\u2010induced damage. Moreover, comet assay studies demonstrated the efficacy of<jats:styled-content style=\"fixed-case\">IV<\/jats:styled-content>08.009 in protecting<jats:styled-content style=\"fixed-case\">DNA<\/jats:styled-content>damage from<jats:styled-content style=\"fixed-case\">UVB<\/jats:styled-content>stress. We found that<jats:styled-content style=\"fixed-case\">IV<\/jats:styled-content>08.009 protects skin from apoptosis induced by oxidative stress,<jats:italic>ex\u00a0vivo<\/jats:italic>. Electron microscopy confirmed the protective efficiency of<jats:styled-content style=\"fixed-case\">IV<\/jats:styled-content>08.009 on cell ultrastructural damage induced by<jats:styled-content style=\"fixed-case\">UVB<\/jats:styled-content>exposure.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Compound<jats:styled-content style=\"fixed-case\">IV<\/jats:styled-content>08.009 demonstrated to be effective in regulating survivin expression and in preserving the basal epidermis from stresses such as<jats:styled-content style=\"fixed-case\">UVB<\/jats:styled-content>and H<jats:sub>2<\/jats:sub>O<jats:sub>2<\/jats:sub>. These results suggest a protective activity of<jats:styled-content style=\"fixed-case\">IV<\/jats:styled-content>08.009 on the essential renewing potential of<jats:styled-content style=\"fixed-case\">KSC<\/jats:styled-content>s.<\/jats:p><\/jats:sec>","DOI":"10.1111\/jocd.12150","type":"journal-article","created":{"date-parts":[[2015,7,14]],"date-time":"2015-07-14T20:48:04Z","timestamp":1436906884000},"page":"191-203","source":"Crossref","is-referenced-by-count":5,"title":["Modulating the expression of survivin and other basal epidermal proteins protects human skin from<scp>UVB<\/scp>damage and oxidative stress"],"prefix":"10.1111","volume":"14","author":[{"given":"Florian","family":"Labarrade","sequence":"first","affiliation":[{"name":"Vincience Global Skin Research Center, Ashland Specialties France Sophia Antipolis France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Laurine","family":"Bergeron","sequence":"additional","affiliation":[{"name":"Vincience Global Skin Research Center, Ashland Specialties France Sophia Antipolis France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Catherine","family":"Serre","sequence":"additional","affiliation":[{"name":"Vincience Global Skin Research Center, Ashland Specialties France Sophia Antipolis France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alexia","family":"Lebleu","sequence":"additional","affiliation":[{"name":"Vincience Global Skin Research Center, Ashland Specialties France Sophia Antipolis France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Val\u00e8re","family":"Busuttil","sequence":"additional","affiliation":[{"name":"Vincience Global Skin Research Center, Ashland Specialties France Sophia Antipolis France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jean\u2010Marie","family":"Botto","sequence":"additional","affiliation":[{"name":"Vincience Global Skin Research Center, Ashland Specialties France Sophia Antipolis France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nouha","family":"Domloge","sequence":"additional","affiliation":[{"name":"Vincience Global Skin Research Center, Ashland Specialties France Sophia Antipolis 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