{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,29]],"date-time":"2026-08-29T00:45:38Z","timestamp":1787964338207,"version":"build-2784847793"},"reference-count":40,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2017,9,14]],"date-time":"2017-09-14T00:00:00Z","timestamp":1505347200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100004484","name":"Tehran University of Medical Sciences and Health Services","doi-asserted-by":"publisher","award":["Grant No. 92\u201003\u201041\u201024652"],"award-info":[{"award-number":["Grant No. 92\u201003\u201041\u201024652"]}],"id":[{"id":"10.13039\/501100004484","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003970","name":"Isfahan University of Medical Sciences","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100003970","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J of Cosmetic Dermatology"],"published-print":{"date-parts":[[2018,6]]},"abstract":"<jats:title>Summary<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Systemic sclerosis (<jats:styled-content style=\"fixed-case\">SS<\/jats:styled-content>c) fibroblasts show resistance apoptosis mechanisms, which enhances the fibrosis stage of the disease. Impaired function of p53 upregulated modulator of apoptosis (<jats:styled-content style=\"fixed-case\">PUMA<\/jats:styled-content>) has been related to deficits in p53\u2010dependant apoptosis pathway. This study aimed to evaluate the transcriptional levels of p53 and <jats:styled-content style=\"fixed-case\">PUMA mRNA<\/jats:styled-content>s in fibroblasts from <jats:styled-content style=\"fixed-case\">SS<\/jats:styled-content>c patients and compare it with healthy individuals.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>In this case\u2010control study, skin biopsy samples were obtained from 19 patients with diffuse cutaneous <jats:styled-content style=\"fixed-case\">SS<\/jats:styled-content>c (Dc<jats:styled-content style=\"fixed-case\">SS<\/jats:styled-content>c) and 16 healthy controls. Afterward, dermal fibroblasts were isolated and cultured. After extraction of total <jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content> from cultured fibroblasts, complementary DNA (<jats:styled-content style=\"fixed-case\">cDNA<\/jats:styled-content>) was synthesized. <jats:styled-content style=\"fixed-case\">mRNA<\/jats:styled-content> quantification was carried out using real\u2010time <jats:styled-content style=\"fixed-case\">PCR<\/jats:styled-content>,<jats:styled-content style=\"fixed-case\"> SYBR<\/jats:styled-content> Green <jats:styled-content style=\"fixed-case\">PCR<\/jats:styled-content> master mix, and specific primers for p53 and PUMA.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>No significant alteration was observed in <jats:styled-content style=\"fixed-case\">mRNA<\/jats:styled-content> expression levels of p53 and <jats:styled-content style=\"fixed-case\">PUMA<\/jats:styled-content> (<jats:italic>P<\/jats:italic>\u00a0=\u00a0.99 and .23, respectively) in fibroblasts from <jats:styled-content style=\"fixed-case\">SS<\/jats:styled-content>c patients compared with controls.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>Apoptosis pathways are impaired in fibroblasts from patients with <jats:styled-content style=\"fixed-case\">SS<\/jats:styled-content>c, leading to chronic fibrosis. Nonetheless, <jats:styled-content style=\"fixed-case\">PUMA<\/jats:styled-content>\/p53 pathway may not be involved in dysfunction of apoptosis mechanisms in fibroblasts of patients with <jats:styled-content style=\"fixed-case\">SS<\/jats:styled-content>c.<\/jats:p><\/jats:sec>","DOI":"10.1111\/jocd.12420","type":"journal-article","created":{"date-parts":[[2017,9,14]],"date-time":"2017-09-14T07:24:44Z","timestamp":1505373884000},"page":"549-554","source":"Crossref","is-referenced-by-count":6,"title":["Expressions of p53 and PUMA in fibroblasts of systemic sclerosis patients are normal at transcription level"],"prefix":"10.1111","volume":"17","author":[{"given":"Mohammad Bagher","family":"Mahmoudi","sequence":"first","affiliation":[{"name":"Rheumatology Research Center Tehran University of Medical Sciences Tehran Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Majid","family":"Abed Khojasteh","sequence":"additional","affiliation":[{"name":"Department of Immunology Faculty of Medicine Isfahan University of Medical Sciences Isfahan Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fereshteh","family":"Alsahebfosoul","sequence":"additional","affiliation":[{"name":"Department of Immunology Faculty of Medicine Isfahan University of Medical Sciences Isfahan Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Farhad","family":"Gharibdoost","sequence":"additional","affiliation":[{"name":"Rheumatology Research Center Tehran University of Medical Sciences Tehran Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shayan","family":"Mostafaei","sequence":"additional","affiliation":[{"name":"Rheumatology Research Center Tehran University of Medical Sciences Tehran Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mazdak","family":"Ganjalikhani\u2010hakemi","sequence":"additional","affiliation":[{"name":"Department of Immunology Faculty of Medicine Isfahan University of Medical Sciences Isfahan Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8164-8831","authenticated-orcid":false,"given":"Mahdi","family":"Mahmoudi","sequence":"additional","affiliation":[{"name":"Rheumatology Research Center Tehran University of Medical Sciences Tehran Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2017,9,14]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"crossref","first-page":"271","DOI":"10.1016\/j.berh.2004.03.001","article-title":"Scleroderma\u2014clinical and pathological advances","volume":"18","author":"Denton CP","year":"2004","journal-title":"Best Pract Res Clin Rheumatol"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(06)68737-0"},{"key":"e_1_2_8_4_1","first-page":"S85","article-title":"Mortality in systemic sclerosis","volume":"26","author":"Karassa F","year":"2008","journal-title":"Clin Exp 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