{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,27]],"date-time":"2026-05-27T11:41:31Z","timestamp":1779882091581,"version":"3.53.1"},"reference-count":34,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2018,10,2]],"date-time":"2018-10-02T00:00:00Z","timestamp":1538438400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J of Cosmetic Dermatology"],"published-print":{"date-parts":[[2019,4]]},"abstract":"Summary<\/jats:title>Background<\/jats:title>The melanocyte and keratinocyte transplantation procedure (MKTP<\/jats:styled-content>) is a safe and effective procedure in treatment of vitiligo. Major advantage of this technique is that a small area of donor skin is needed to cover a large recipient area. To date, there is no consensus on the optimal donor\u2010to\u2010recipient ratio (D\/R) required to achieve acceptable repigmentation following melanocyte and keratinocyte transplantation procedure (MKTP<\/jats:styled-content>) in generalized vitiligo. It has been postulated that the addition of post\u2010transplantation phototherapy may enhance the results. This is first study to assess two different (D\/R) ratios with or without adjuvant phototherapy.<\/jats:p><\/jats:sec>Objective<\/jats:title>To compare the repigmentation after MKTP<\/jats:styled-content> using two different D\/R ratios (1\/3 and 1\/10) with and without adjuvant phototherapy (NB<\/jats:styled-content>\u2010UVB<\/jats:styled-content>).<\/jats:p><\/jats:sec>Methods and materials<\/jats:title>In this non randomized prospective clinical trial, 42 patients with stable generalized vitiligo bilateral and symmetrical in distribution were included. Patients were divided into two groups, 21 patients with a total of 50 lesions were treated with MKTP<\/jats:styled-content> using a D\/R ratio of 1\/3 (Group I; 3000\u00a0\u00b1\u00a0500\u00a0cell\/mm2<\/jats:sup>) and the other 21 patients with a total of 52 lesions were treated by MKTP<\/jats:styled-content> using a D\/R ratio of 1\/10 (Group II<\/jats:styled-content>; 1000\u00a0\u00b1\u00a0200 cell\/mm2<\/jats:sup>). To study the role of adjuvant phototherapy on repigmentation, lesions in each patient were divided into two subgroups (a and b): lesions in subgroups Ia and II<\/jats:styled-content>a (did not receive NB<\/jats:styled-content>\u2010UVB<\/jats:styled-content>) and lesions in subgroups Ib and II<\/jats:styled-content>b (received adjuvant phototherapy NB<\/jats:styled-content>\u2010UVB<\/jats:styled-content>, two sessions per week for 6\u00a0months). The overall grading of repigmentation used was excellent (90%\u2010100% repigmentation), good (75%\u201089%), fair (50%\u201074%), and poor (<50%). Also, the percentage of VASI<\/jats:styled-content> change and color matching were used to assess the results. The study design was approved by the ethical committee of the Faculty of Medicine, Assiut University (IRB<\/jats:styled-content> attached).<\/jats:p><\/jats:sec>Results<\/jats:title>The mean percentage of repigmentation was significantly better in group I than group II<\/jats:styled-content> cases in both areas with or without adjuvant NB<\/jats:styled-content>\u2010UVB<\/jats:styled-content>. It was 86.00\u00a0\u00b1\u00a016.21 and 87.62\u00a0\u00b1\u00a011.66 in subgroups Ia and Ib, respectively, vs 24.14\u00a0\u00b1\u00a018.08 and 29.98\u00a0\u00b1\u00a016.34 in subgroups II<\/jats:styled-content>a and II<\/jats:styled-content>b, respectively (P<\/jats:italic> value was 0.000). The percent of excellent response was significantly better in group I than group II<\/jats:styled-content>. It was 60% and 64% in subgroups Ia and Ib, respectively, and 7.6 and 11.5 in subgroups II<\/jats:styled-content>a and II<\/jats:styled-content>b, respectively (P<\/jats:italic> value was 0.000). The mean percentage of VASI<\/jats:styled-content> change was significantly better in group I than group II<\/jats:styled-content> cases in both areas. It was \u221290.74\u00a0\u00b1\u00a015.84 and \u221292.06\u00a0\u00b1\u00a011.86 in subgroups Ia and Ib, respectively, vs \u221223.10\u00a0\u00b1\u00a032.85 and \u221226.03\u00a0\u00b1\u00a035.15 in subgroups II<\/jats:styled-content>a and II<\/jats:styled-content>b, respectively (P<\/jats:italic> value was 0.000). The percent of excellent color match was better in group I than group II<\/jats:styled-content>. It was 84% and 88% in subgroups Ia and Ib, respectively, vs 34.6 in both subgroups II<\/jats:styled-content>a and II<\/jats:styled-content>b (P<\/jats:italic>\u00a0<\u00a00.05). A higher density of epidermal cells was transplanted in the recipient area in group I (3000\u00a0\u00b1\u00a0500\u00a0cell\/mm2<\/jats:sup>) compared to group II<\/jats:styled-content> (1000\u00a0\u00b1\u00a0200 cell\/mm2<\/jats:sup>). There were no statistically significant differences between subgroups (Ia vs Ib and II<\/jats:styled-content>a vs II<\/jats:styled-content>b) although percentage of repigmentation was slightly better in NB<\/jats:styled-content>\u2010UVB<\/jats:styled-content> subgroups.<\/jats:p><\/jats:sec>Conclusions<\/jats:title>The higher density of epidermal cells used in the suspension, the higher the percentage of repigmentation obtained. The usage of adjuvant phototherapy following NKMT<\/jats:styled-content> can enhance the repigmentation response.<\/jats:p><\/jats:sec>","DOI":"10.1111\/jocd.12759","type":"journal-article","created":{"date-parts":[[2018,10,3]],"date-time":"2018-10-03T00:51:09Z","timestamp":1538527869000},"page":"638-646","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":29,"title":["The effect of NB<\/scp>\u2010UVB<\/scp> on noncultured melanocyte and keratinocyte transplantation in treatment of generalized vitiligo using two different donor\u2010to\u2010recipient ratios"],"prefix":"10.1111","volume":"18","author":[{"given":"Yasmin Mostafa","family":"Tawfik","sequence":"first","affiliation":[{"name":"Department of Dermatology, Venereology and Andrology Faculty of Medicine, Plastic Surgery and Pathology Departments Assiut University Assiut Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nagwa Essa","family":"Abd Elazim","sequence":"additional","affiliation":[{"name":"Department of Dermatology, Venereology and Andrology Faculty of Medicine, Plastic Surgery and Pathology Departments Assiut University Assiut Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Amira Ali","family":"Abdel\u2010Motaleb","sequence":"additional","affiliation":[{"name":"Department of Dermatology, Venereology and Andrology Faculty of Medicine, Plastic Surgery and Pathology Departments Assiut University Assiut Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rabab Ahmed Ahmed","family":"Mohammed","sequence":"additional","affiliation":[{"name":"Department of Dermatology, Venereology and Andrology Faculty of Medicine, Plastic Surgery and Pathology Departments Assiut University Assiut Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ahmed Mohamed Ali","family":"Tohamy","sequence":"additional","affiliation":[{"name":"Department of Dermatology, Venereology and Andrology Faculty of Medicine, Plastic Surgery and Pathology Departments Assiut University Assiut Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2018,10,2]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1111\/dsu.12444"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1468-3083.2010.03759.x"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-4632.2004.02403.x"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1111\/exd.12344"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/0190-9622(92)70024-A"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2133.1998.02177.x"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1001\/archderm.140.10.1211"},{"key":"e_1_2_6_9_1","first-page":"755","article-title":"Narrow band ultraviolet B phototherapy in combination with other therapies for vitiligo: mechanism and efficacies","volume":"13","author":"Yazandi A","year":"2014","journal-title":"J Eur Acad Dermatol Venereol"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.1001\/archderm.140.6.677"},{"key":"e_1_2_6_11_1","first-page":"313","article-title":"Melanocyte\u2010keratinocyte transplantation procedure in the treatment of vitiligo: the experience of an academic medical centre in the United States","volume":"68","author":"Huggins R","year":"2011","journal-title":"J Am Acad Dermatol"},{"issue":"1","key":"e_1_2_6_12_1","first-page":"33","article-title":"A novel scoring system for evaluation of results of autologous transplantation methods in vitiligo","volume":"68","author":"Gupta S","year":"2002","journal-title":"Indian J Dermatol Venereol Leprol"},{"key":"e_1_2_6_13_1","doi-asserted-by":"publisher","DOI":"10.1111\/dth.12007"},{"key":"e_1_2_6_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaad.2010.11.061"},{"key":"e_1_2_6_15_1","doi-asserted-by":"publisher","DOI":"10.1111\/bjd.12725"},{"key":"e_1_2_6_16_1","doi-asserted-by":"publisher","DOI":"10.1111\/bjd.12532"},{"key":"e_1_2_6_17_1","unstructured":"SalahS AnbarT Tarek El\u2010SayedE YasserME.Some factors affecting surgery in Vitiligo\u201cMD Thesis\u201d.Dermatology Venereology and Andrology Faculty of Medicine Minia University;2015."},{"key":"e_1_2_6_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaad.2011.05.002"},{"key":"e_1_2_6_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaad.2017.01.056"},{"key":"e_1_2_6_20_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1524-4725.2001.01045.x"},{"key":"e_1_2_6_21_1","doi-asserted-by":"publisher","DOI":"10.1111\/bjd.12325"},{"key":"e_1_2_6_22_1","doi-asserted-by":"publisher","DOI":"10.1111\/bjd.12007"},{"key":"e_1_2_6_23_1","doi-asserted-by":"publisher","DOI":"10.1038\/jid.2015.178"},{"key":"e_1_2_6_24_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2133.2010.10014.x"},{"key":"e_1_2_6_25_1","doi-asserted-by":"publisher","DOI":"10.1001\/archderm.134.12.1532"},{"key":"e_1_2_6_26_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1468-3083.2010.03946.x"},{"key":"e_1_2_6_27_1","doi-asserted-by":"publisher","DOI":"10.1586\/edm.10.9"},{"key":"e_1_2_6_28_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-4632.2005.02226.x"},{"key":"e_1_2_6_29_1","first-page":"1203","article-title":"Double\u2010blind placebo controlled study of autologous transplanted epidermal cell suspensions for repigmenting vitiligo","volume":"140","author":"Geel N","year":"2004","journal-title":"Arch Dermatol"},{"key":"e_1_2_6_30_1","doi-asserted-by":"publisher","DOI":"10.1159\/000092833"},{"key":"e_1_2_6_31_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1524-4725.2007.33216.x"},{"key":"e_1_2_6_32_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1524-4725.2010.01477.x"},{"key":"e_1_2_6_33_1","doi-asserted-by":"publisher","DOI":"10.1177\/0300060513516294"},{"key":"e_1_2_6_34_1","doi-asserted-by":"publisher","DOI":"10.1111\/phpp.12104"},{"key":"e_1_2_6_35_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.mjafi.2013.09.004"}],"container-title":["Journal of Cosmetic Dermatology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fjocd.12759","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/jocd.12759","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/jocd.12759","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/jocd.12759","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,10]],"date-time":"2023-09-10T23:56:58Z","timestamp":1694390218000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/jocd.12759"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2018,10,2]]},"references-count":34,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2019,4]]}},"alternative-id":["10.1111\/jocd.12759"],"URL":"https:\/\/doi.org\/10.1111\/jocd.12759","archive":["Portico"],"relation":{},"ISSN":["1473-2130","1473-2165"],"issn-type":[{"value":"1473-2130","type":"print"},{"value":"1473-2165","type":"electronic"}],"subject":[],"published":{"date-parts":[[2018,10,2]]},"assertion":[{"value":"2018-07-20","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2018-10-02","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}