{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,17]],"date-time":"2026-09-17T20:27:17Z","timestamp":1789676837839,"version":"4.0.1"},"reference-count":25,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2018,11,9]],"date-time":"2018-11-09T00:00:00Z","timestamp":1541721600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J of Cosmetic Dermatology"],"published-print":{"date-parts":[[2019,8]]},"abstract":"<jats:title>Summary<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Acne vulgaris is a common skin disease characterized by increased sebum production, inflammation, and colonization of <jats:italic>Propionibacterium acnes<\/jats:italic> (<jats:italic>P.\u00a0acnes<\/jats:italic>) on pilosebaceous follicles.<\/jats:p><\/jats:sec><jats:sec><jats:title>Aims<\/jats:title><jats:p>To determine the efficacy of two different plant extracts against <jats:italic>P.\u00a0acnes<\/jats:italic> and to analyze the gene expression levels of IL\u20101\u03b1, SRD5A1, and TNF\u03b1 in HaCaT cells treated with these plant extracts.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Anti\u2010acne extract 1 (AE1) consisted of <jats:italic>Juglans regia<\/jats:italic> (walnut husk), <jats:italic>Myrtus communis<\/jats:italic> (myrtle leaves), <jats:italic>Matricaria chamomilla<\/jats:italic> (chamomilla flowers), <jats:italic>Urtica dioica<\/jats:italic> (stinging nettle leaves), and <jats:italic>Rosa damascena<\/jats:italic> (rose flowers). Anti\u2010acne extract 2 (AE2) contained <jats:italic>Brassica oleracea<\/jats:italic> var. <jats:italic>botrytis<\/jats:italic> (broccoli) and <jats:italic>B.\u00a0oleracea<\/jats:italic> var. <jats:italic>italica<\/jats:italic> (cauliflower). The antimicrobial activities of the extracts were tested on two different <jats:italic>P.\u00a0acnes<\/jats:italic> strains: the reference strain of <jats:italic>P.\u00a0acnes<\/jats:italic> (ATCC 51277) and the clinical isolate from a patient. The minimum inhibitory concentration (MIC) of the extracts was determined using the broth dilution method. Human keratinocyte cells were used for in vitro tests. Gene expression analyses were performed with RT\u2010qPCR.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>The MIC values of the extracts were below 1\/2048\u00a0\u00b5g\/mL. In the gene expression analysis, AE1 increased the expression level of TNF\u03b1 (1.1719, <jats:italic>P<\/jats:italic>\u00a0&lt;\u00a00.0001), suppressed the expression level of IL\u20101\u03b1, SRD5A1 (0.0588, <jats:italic>P<\/jats:italic>\u00a0=\u00a00.0231; 0.3081, <jats:italic>P<\/jats:italic>\u00a0=\u00a00.0351), respectively. AE2 suppressed gene expression level of IL\u20101\u03b1, SRD5A1, TNF\u03b1 (0.3815, <jats:italic>P<\/jats:italic>\u00a0=\u00a00.0254; 0.3418, <jats:italic>P<\/jats:italic>\u00a0=\u00a00.0271; 0.1997, <jats:italic>P<\/jats:italic>\u00a0=\u00a00.0623).<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>Both herbal extracts demonstrated strong antibacterial and anti\u2010inflammatory activity in this preliminary trial. In conclusion, the topical application of these botanical extracts can be good candidates for local acne treatment.<\/jats:p><\/jats:sec>","DOI":"10.1111\/jocd.12814","type":"journal-article","created":{"date-parts":[[2018,11,10]],"date-time":"2018-11-10T06:13:03Z","timestamp":1541830383000},"page":"1061-1065","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":25,"title":["Efficacy of two plant extracts against acne vulgaris: Initial results of microbiological tests and cell culture studies"],"prefix":"10.1111","volume":"18","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-5299-712X","authenticated-orcid":false,"given":"Song\u00fcl","family":"K\u0131l\u0131\u00e7","sequence":"first","affiliation":[{"name":"Biota Research &amp; Development Center Istanbul Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sinem \u00d6ktem","family":"Okullu","sequence":"additional","affiliation":[{"name":"Department of 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