{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T16:52:10Z","timestamp":1790700730463,"version":"4.1.0"},"reference-count":37,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2019,5,10]],"date-time":"2019-05-10T00:00:00Z","timestamp":1557446400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"Mantecorp Ind\u00fastria Qu\u00edmica e Farmac\u00eautica Ltda,\u00a0S\u00e3o Paulo-SP, Brazil"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J of Cosmetic Dermatology"],"published-print":{"date-parts":[[2020,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Melanin plays an important role in protecting the skin against the harmful effects of solar radiation, but its abnormal accumulation may become an aesthetic problem, such as melasma and age spots.<\/jats:p><\/jats:sec><jats:sec><jats:title>Aims<\/jats:title><jats:p>The aim of this study was to evaluate the antiangiogenic and whitening effects of a depigmentation formulation (<jats:styled-content style=\"fixed-case\">BLTX<\/jats:styled-content>) using an in vitro model of human cell and skin culture.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Human fibroblasts, keratinocytes or melanocytes were treated with <jats:styled-content style=\"fixed-case\">BLTX<\/jats:styled-content> and subjected to oxidative stress by <jats:styled-content style=\"fixed-case\">UV<\/jats:styled-content> radiation or inflammatory stress with <jats:styled-content style=\"fixed-case\">IL<\/jats:styled-content>\u20101\u03b1 for quantification of melanin, tyrosinase, endothelin\u20101, <jats:styled-content style=\"fixed-case\">PAR<\/jats:styled-content>\u20102, <jats:styled-content style=\"fixed-case\">VEGF<\/jats:styled-content> and <jats:styled-content style=\"fixed-case\">iNOS<\/jats:styled-content>. Fragments of human skin, from elective plastic surgery, were treated with <jats:styled-content style=\"fixed-case\">BLTX<\/jats:styled-content> and subjected to histological evaluation with hematoxylin\/eosin associated with Fontana\u2010Masson technique for melanin view. A parametric method, the one\u2010way analysis of variance (<jats:styled-content style=\"fixed-case\">ANOVA<\/jats:styled-content>) followed by the Bonferroni test, was used to compare data among all groups.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>The results demonstrated that <jats:styled-content style=\"fixed-case\">BLTX<\/jats:styled-content> promotes a reduction in <jats:styled-content style=\"fixed-case\">VEGF<\/jats:styled-content> and <jats:styled-content style=\"fixed-case\">iNOS<\/jats:styled-content> protein synthesis in cultured dermal fibroblasts, indicating an antiangiogenic property. In relation to whitening effect, <jats:styled-content style=\"fixed-case\">BLTX<\/jats:styled-content> was able to reduce the production of melanin in both systems, melanocytes and human skin cultures. The depigmenting action was also revealed by decreasing the levels of endothelin\u20101, <jats:styled-content style=\"fixed-case\">PAR<\/jats:styled-content>\u20102 and activity of tyrosinase, when compared to cultures exposed to <jats:styled-content style=\"fixed-case\">UV<\/jats:styled-content> radiation.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>The results allow us to infer that <jats:styled-content style=\"fixed-case\">BLTX<\/jats:styled-content> presents an antiangiogenic effect indicating a role in the vascular component of melasma. 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