{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,24]],"date-time":"2026-06-24T01:53:10Z","timestamp":1782265990651,"version":"3.54.5"},"reference-count":23,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2019,7,25]],"date-time":"2019-07-25T00:00:00Z","timestamp":1564012800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J of Cosmetic Dermatology"],"published-print":{"date-parts":[[2020,4]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Standardized skin surface biopsy (SSSB) is often performed to determine the density of <jats:italic>Demodex<\/jats:italic> mites in facial papulopustular eruptions.<\/jats:p><\/jats:sec><jats:sec><jats:title>Aim<\/jats:title><jats:p>We aimed to test the applicability of a new, \u201csuperficial needle\u2010scraping\u201d (SNS) method for assessing <jats:italic>Demodex<\/jats:italic> density in papulopustular rosacea (PPR).<\/jats:p><\/jats:sec><jats:sec><jats:title>Patients and methods<\/jats:title><jats:p>Using SNS method, we measured the <jats:italic>Demodex<\/jats:italic> density in patients with PPR, also enrolling the patients with acne vulgaris as controls. SNS was performed by gently scraping off 5 small pustules with the convex surface of the tip of an 18# needle for examination. For comparison, SSSB was also performed in patients with PPR. <jats:italic>Demodex<\/jats:italic> density was expressed as \u201cmites per 5 pustules\u201d for SNS and as \u201cmites per cm<jats:sup>2<\/jats:sup>\u201d for SSSB.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>A total of 40 patients with PPR and 35 patients with acne vulgaris were recruited. There were no statistically significant differences in age or sex between the PPR and acne groups. The <jats:italic>Demodex<\/jats:italic> density was 5.6\u00a0\u00b1\u00a04.2 in the PPR group versus 0.3\u00a0\u00b1\u00a01.0 in the acne group (<jats:italic>P<\/jats:italic>\u00a0&lt;\u00a0.001). The cutoff of \u201c\u22653 <jats:italic>Demodex<\/jats:italic> mites per 5 pustules\u201d gave a sensitivity of 78% and a specificity of 97%, and the area under the receiver operating characteristic curve was 0.89. Moreover, SNS and SSSB gave mutually concordant results (positive or negative) in half of the patients.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Our study suggests that SNS is a simple and convenient method for assessing <jats:italic>Demodex<\/jats:italic> density of pustules in PPR and can be a useful alternative or addition to SSSB for evaluation of Demodex\u2010associated facial papulopustular eruptions.<\/jats:p><\/jats:sec>","DOI":"10.1111\/jocd.13082","type":"journal-article","created":{"date-parts":[[2019,7,25]],"date-time":"2019-07-25T12:42:26Z","timestamp":1564058546000},"page":"896-900","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":16,"title":["A new superficial needle\u2010scraping method for assessing <i>Demodex<\/i> density in papulopustular rosacea"],"prefix":"10.1111","volume":"19","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-5902-9532","authenticated-orcid":false,"given":"Hui\u2010Peng","family":"Huang","sequence":"first","affiliation":[{"name":"Huang Dermatology Clinic Tainan Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4365-4533","authenticated-orcid":false,"given":"Chao\u2010Kai","family":"Hsu","sequence":"additional","affiliation":[{"name":"Department of Dermatology National Cheng Kung University Hospital College of Medicine National Cheng Kung University Tainan Taiwan"},{"name":"International Research Center of Wound Repair and Regeneration (iWRR) National Cheng Kung University Tainan Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6777-7357","authenticated-orcid":false,"given":"Julia Yu\u2010Yun","family":"Lee","sequence":"additional","affiliation":[{"name":"Department of Dermatology National Cheng Kung University Hospital College of Medicine National Cheng Kung University Tainan Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2019,7,25]]},"reference":[{"key":"e_1_2_5_2_1","doi-asserted-by":"publisher","DOI":"10.2307\/3278267"},{"key":"e_1_2_5_3_1","doi-asserted-by":"publisher","DOI":"10.1080\/000155502753600795"},{"key":"e_1_2_5_4_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1442-9071.1991.tb00666.x"},{"key":"e_1_2_5_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0379-0738(03)00216-0"},{"key":"e_1_2_5_6_1","doi-asserted-by":"publisher","DOI":"10.1001\/archderm.1961.01580110104016"},{"key":"e_1_2_5_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaad.2008.10.058"},{"key":"e_1_2_5_8_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2133.1993.tb00261.x"},{"key":"e_1_2_5_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/0190-9622(93)70065-2"},{"key":"e_1_2_5_10_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1468-3083.2001.00331.x"},{"key":"e_1_2_5_11_1","doi-asserted-by":"publisher","DOI":"10.12970\/2310-998X.2014.02.01.3"},{"key":"e_1_2_5_12_1","doi-asserted-by":"publisher","DOI":"10.1080\/00015550510030069"},{"key":"e_1_2_5_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0190-9622(86)70287-9"},{"key":"e_1_2_5_14_1","first-page":"321","article-title":"Resistant scalp folliculitis secondary to Demodex infestation","volume":"76","author":"Sanfilippo AM","year":"2005","journal-title":"Cutis"},{"key":"e_1_2_5_15_1","unstructured":"VarottiC NegosantiM PassariniBDemodex folliculorumed acne rosacea. 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