{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,4]],"date-time":"2026-06-04T14:10:04Z","timestamp":1780582204062,"version":"3.54.1"},"reference-count":33,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2020,2,14]],"date-time":"2020-02-14T00:00:00Z","timestamp":1581638400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"FUNADERSP","award":["008\/2013"],"award-info":[{"award-number":["008\/2013"]}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J of Cosmetic Dermatology"],"published-print":{"date-parts":[[2020,10]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Diabetic cutaneous ulcers are subjected to several physiological and biochemical defects, which contribute to wound chronicity and therapeutic failure. Platelet\u2010rich plasma (PRP) has been used for stimulating tissue regeneration, and mesenchymal stromal cells (MSCs) have demonstrated therapeutic properties in all phases of skin regeneration in cell therapy studies.<\/jats:p><\/jats:sec><jats:sec><jats:title>Aims<\/jats:title><jats:p>The objective of this study was to evaluate the therapeutic effects related to the use of a biomembrane composed of autologous MSCs and PRP on chronic wounds of diabetic patients (pre\u2010post pilot study).<\/jats:p><\/jats:sec><jats:sec><jats:title>Patients\/Methods<\/jats:title><jats:p>Six diabetic patients with chronic wounds for more than 6\u00a0months were subjected to adipose tissue collection for isolation of MSCs, blood collection for PRP preparation, and topical administration of a biomembrane of MSCs and PRP on each chronic wound. The statistical difference regarding the evolution of ulcers was calculated by means of paired <jats:italic>t<\/jats:italic> test.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>There was granulation tissue formation starting from 7\u00a0days after topical application. Total re\u2010epithelialization occurred in 5 of the 9 lesions treated, and the mean wound healing rate (WHR) was 74.55% (\u00b132.55%) after 90\u00a0days. No cicatricial hypertrophy or retraction was observed.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Mesenchymal stromal cells topical therapy associated with PRP is well\u2010tolerated and able to provide a reduction in ulcer area of diabetic chronic wounds.<\/jats:p><\/jats:sec>","DOI":"10.1111\/jocd.13321","type":"journal-article","created":{"date-parts":[[2020,2,15]],"date-time":"2020-02-15T05:50:20Z","timestamp":1581745820000},"page":"2669-2678","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":15,"title":["A topical cell therapy approach for diabetic chronic ulcers: Effects of mesenchymal stromal cells associated with platelet\u2010rich plasma"],"prefix":"10.1111","volume":"19","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-4254-209X","authenticated-orcid":false,"given":"Talita","family":"Stessuk","sequence":"first","affiliation":[{"name":"University of S\u00e3o Paulo, USP S\u00e3o Paulo Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7645-5891","authenticated-orcid":false,"given":"Jo\u00e3o Tadeu","family":"Ribeiro\u2010Paes","sequence":"additional","affiliation":[{"name":"S\u00e3o Paulo State University UNESP\u2013Campus de Assis Assis Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Paula Tavares","family":"Colpas","sequence":"additional","affiliation":[{"name":"University of Campinas UNICAMP Campinas Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Paulo C\u00e9sar","family":"Martins Alves","sequence":"additional","affiliation":[{"name":"University of Campinas UNICAMP Campinas Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jussara","family":"Rehder","sequence":"additional","affiliation":[{"name":"University of Campinas UNICAMP Campinas Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carla Aparecida Faccio","family":"Bosnardo","sequence":"additional","affiliation":[{"name":"University of Campinas UNICAMP Campinas Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ana Terezinha","family":"Guillaumon","sequence":"additional","affiliation":[{"name":"University of Campinas UNICAMP Campinas Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andresa","family":"Forte","sequence":"additional","affiliation":[{"name":"CordCell CellTherapy S\u00e3o Paulo Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8248-7884","authenticated-orcid":false,"given":"Maria Beatriz","family":"Puzzi","sequence":"additional","affiliation":[{"name":"University of Campinas UNICAMP Campinas Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2020,2,14]]},"reference":[{"key":"e_1_2_11_2_1","unstructured":"World Health Organization.WHO global report on diabetes. 2016. 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