{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,19]],"date-time":"2026-10-19T20:01:45Z","timestamp":1792440105645,"version":"4.5.0"},"reference-count":41,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2021,4,25]],"date-time":"2021-04-25T00:00:00Z","timestamp":1619308800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100013041","name":"Vice-Chancellor for Research, Shiraz University of Medical Sciences","doi-asserted-by":"publisher","award":["6604"],"award-info":[{"award-number":["6604"]}],"id":[{"id":"10.13039\/501100013041","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J of Cosmetic Dermatology"],"published-print":{"date-parts":[[2021,12]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Skin wound healing has always been a challenging subject as it involves the coordinated functioning of various cells and molecules. Any disorder in wound healing can cause healing failure and result in chronic wounds. In this study, we hypothesized that co\u2010cultured dermal fibroblasts (DFs) and Wharton's jelly mesenchymal stem cells (WJ\u2010MSCs) seeded on an acellular amniotic membrane scaffold could be used to promote skin regeneration in chronic ulcers.<\/jats:p><\/jats:sec><jats:sec><jats:title>Materials and Methods<\/jats:title><jats:p>In this case series, the chronic wounds of five diabetic patients aged between 30 and 60\u00a0years were treated with co\u2010cultured WJ\u2010MSCs and DFs seeded on an acellular amniotic membrane. Treatment was applied and the wound healing process was evaluated every three days for nine days, with the patients being subsequently followed up for one month. The wound healing percentage, time taken for the wound to heal, and wound size were monitored.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>The mean wound healing rate (WHR) increased progressively in all lesions. The mean percentage of wound healing after transplantation of the biological scaffold enriched with WJ\u2010MSCs and autologous DFs after treatment was 93.92%, respectively. The healing percentage significantly increased after three days; significant decreases in wound size and healing time were recorded after six and nine days of treatment, respectively (<jats:italic>p<\/jats:italic>\u00a0&lt;\u00a00.002); and total skin regeneration and re\u2010epithelialization were achieved by the ninth day of treatment. There were no side effects or complications.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Given the current problems and complications presented by chronic wounds, Novel Clinical approaches involving cell therapy and tissue engineering can be regarded as an attractive therapeutic option for the treatment of chronic and difficult\u2010to\u2010heal wounds.<\/jats:p><\/jats:sec>","DOI":"10.1111\/jocd.14043","type":"journal-article","created":{"date-parts":[[2021,3,3]],"date-time":"2021-03-03T16:56:21Z","timestamp":1614790581000},"page":"4040-4047","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":35,"title":["Effect of dermal fibroblasts and mesenchymal stem cells seeded on an amniotic membrane scaffold in skin regeneration: A case series"],"prefix":"10.1111","volume":"20","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-9395-9573","authenticated-orcid":false,"given":"Seyedeh\u2010Sara","family":"Hashemi","sequence":"first","affiliation":[{"name":"Burn and Wound Healing Research Center Shiraz University of Medical Sciences Shiraz Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ali Akbar","family":"Mohammadi","sequence":"additional","affiliation":[{"name":"Burn and Wound Healing Research Center Shiraz University of Medical Sciences Shiraz Iran"},{"name":"Division of Plastic and Reconstructive Surgery Department of Surgery Shiraz University of Medical Sciences Shiraz Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kasra","family":"Moshirabadi","sequence":"additional","affiliation":[{"name":"Division of Plastic and Reconstructive Surgery Department of Surgery Shiraz University of Medical Sciences Shiraz 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