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Total protein content was measured by the Biuret method and antioxidant activity by the 2,2\u2010diphenyl\u20101\u2010picryl hydrazyl (DPPH) assay. Fourier transform infrared spectroscopy (FTIR) analysis was used to determine spectral signatures and biocompatibility was carried out by the Neutral Red Uptake (NRU) and Sulforhodamine B (SRB) assays. <jats:italic>In vivo<\/jats:italic> safety and efficacy was evaluated in an experimental pilot study on 15 volunteers.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>The <jats:italic>in vitro<\/jats:italic> results confirmed stability in the protein content (7\u00a0mg\/ml), antioxidant activity (49.50%), and FTIR fingerprints of the MSC\u2010CM. In the biocompatibility experiments by both SRB and NRU methods, no IC<jats:sub>50<\/jats:sub> value could be derived at 100% concentration indicating safety at the cellular level. The <jats:italic>in vivo<\/jats:italic> results indicated safety with no side effects or adverse reactions, while 86.6% of the subjects experienced a positive effect of hair regeneration.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>MSC\u2010CM comprises a rich cocktail of physiologically balanced growth factors, cytokines, and beneficial proteins which may explain the bioactivity and mechanism of action in hair regrowth. This may indicate a biocompatible, gentle, and safe regenerative approach to address hair loss.<\/jats:p><\/jats:sec>","DOI":"10.1111\/jocd.14114","type":"journal-article","created":{"date-parts":[[2021,3,29]],"date-time":"2021-03-29T17:52:10Z","timestamp":1617040330000},"page":"740-749","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":10,"title":["In vitro and clinical evaluation of umbilical cord\u2010derived mesenchymal stromal cell\u2010conditioned media for hair regeneration"],"prefix":"10.1111","volume":"21","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-6324-9845","authenticated-orcid":false,"given":"Caroline","family":"Mathen","sequence":"first","affiliation":[{"name":"Clinical R &amp; D OCT Therapies and Research Pvt Ltd Mumbai India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wilfrid","family":"Dsouza","sequence":"additional","affiliation":[{"name":"Clinical R &amp; D OCT Therapies and 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