{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,20]],"date-time":"2026-07-20T21:44:29Z","timestamp":1784583869538,"version":"3.55.0"},"reference-count":16,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2021,10,25]],"date-time":"2021-10-25T00:00:00Z","timestamp":1635120000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"DOI":"10.13039\/100004368","name":"Colgate-Palmolive Company","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100004368","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J of Cosmetic Dermatology"],"published-print":{"date-parts":[[2022,7]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Amino acids are major components of skin's natural moisturizing factors and play a role in regulating skin hydration and skin pH.<\/jats:p><\/jats:sec><jats:sec><jats:title>Objective<\/jats:title><jats:p>This research examines a proprietary amino acid complex technology (AAComplex) designed to help reduce skin irritation and repair skin damage.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:sec><jats:label\/><jats:p>\n<jats:italic>In\u2010 vitro Scratch Assay<\/jats:italic>\n<\/jats:p><jats:p>HaCaT cells are scratched, and the wounds are imaged at different time points until the closure of the scratch wound is detected.<\/jats:p><\/jats:sec><jats:sec><jats:label\/><jats:p>\n<jats:italic>In\u2010vitro 3D Reconstructed Human Tissue Evaluation<\/jats:italic>\n<\/jats:p><jats:p>The concentration of heat shock protein 27 (HSP\u201027) extracted from 3D reconstructed human skin equivalent tissues and IL\u20101a released to the media was determined using enzyme\u2010linked immunosorbent assays.<\/jats:p><\/jats:sec><jats:sec><jats:label\/><jats:p>\n<jats:italic>In Vivo Clinical Study<\/jats:italic>\n<\/jats:p><jats:p>37 subjects were enrolled in a split\u2010face study design. On test days 1, 2, 4, and 8, subjects visited the test facility to have their face assessed by facial swabbing and bio\u2010instrumentation measurements.<\/jats:p><\/jats:sec><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:sec><jats:label\/><jats:p>\n<jats:bold>\n<jats:italic>In\u2010 vitro Scratch Assay<\/jats:italic>\n<\/jats:bold>\n<\/jats:p><jats:p>The AAComplex demonstrated a strong cell renewal benefit in the HaCaT (human) cells scratch assay.<\/jats:p><\/jats:sec><jats:sec><jats:label\/><jats:p>\n<jats:italic>In Vitro 3D Reconstructed Human Tissue Evaluation<\/jats:italic>\n<\/jats:p><jats:p>AAComplex demonstrated a significant skin repair benefit by quantifying Heat Shock Protein, HSP\u201027. Induced skin irritation was significantly reduced by quantifying interleukin\u20101 alpha biomarker, IL\u20101a.<\/jats:p><\/jats:sec><jats:sec><jats:label\/><jats:p>\n<jats:italic>In Vivo Clinical Study<\/jats:italic>\n<\/jats:p><jats:p>The test products delivered skin benefits by reducing visual redness and skin irritation while increasing moisturization.<\/jats:p><\/jats:sec><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>The <jats:italic>in vitro<\/jats:italic> and <jats:italic>in vivo<\/jats:italic> clinical studies demonstrated that the AAComplex technology formulated in the commercially available Skin Recovery System effectively reduced skin irritation and redness as well as accelerating the skin repair process.<\/jats:p><\/jats:sec>","DOI":"10.1111\/jocd.14544","type":"journal-article","created":{"date-parts":[[2021,10,25]],"date-time":"2021-10-25T15:08:21Z","timestamp":1635174501000},"page":"3046-3052","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":23,"title":["Amino acid complex (AAComplex) benefits in cosmetic products: <i>In vitro<\/i> and <i>in vivo<\/i> clinical studies"],"prefix":"10.1111","volume":"21","author":[{"given":"Isabel","family":"Diaz","sequence":"first","affiliation":[{"name":"Dermal Clinical Colgate\u2010Palmolive  Piscataway New Jersey USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jin","family":"Namkoong","sequence":"additional","affiliation":[{"name":"Early Research Colgate\u2010Palmolive  Piscataway New Jersey USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Joanna Qiang","family":"Wu","sequence":"additional","affiliation":[{"name":"Early Research 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