{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,22]],"date-time":"2026-04-22T11:04:52Z","timestamp":1776855892128,"version":"3.51.2"},"reference-count":17,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2022,1,2]],"date-time":"2022-01-02T00:00:00Z","timestamp":1641081600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J of Cosmetic Dermatology"],"published-print":{"date-parts":[[2022,8]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Platelet\u2010rich plasma (PRP) injection is a promising modality for hair regeneration in female pattern hair loss (FPHL). A standard protocol on best methods for PRP preparation has not been established.<\/jats:p><\/jats:sec><jats:sec><jats:title>Objectives<\/jats:title><jats:p>To optimize standard PRP preparation protocols and evaluate its clinical efficacy in FPHL.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Comparative study enrolled 40 female patients with FPHL divided randomly into 4 equal groups. Each group received 3 sessions of monthly intradermal injection of PRP prepared by different methods regarding number of spins, centrifugation speeds, type of the centrifuge, and the size of PRP tube. Patients were evaluated by trichoscan before and 1\u00a0month after the 3<jats:sup>rd<\/jats:sup> session for number of terminal, vellus hair, and average hair width.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>A statistically significant increase in platelet count in PRP prepared by combination of digital centrifuge, large\u2010sized sodium citrate tube, and low centrifugation speed (900\u00a0rpm). All patients showed statistically significant increase in percentage of terminal hair and average width of hair after treatment as assessed by trichoscan, without statistically significant difference between studied groups.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>Digital centrifuge, large\u2010sized sodium citrate tubes, and a single spin with low centrifugation speed (900\u00a0rpm) were ideal for PRP preparation. PRP is an effective and safe modality in FPHL therapy.<\/jats:p><\/jats:sec>","DOI":"10.1111\/jocd.14648","type":"journal-article","created":{"date-parts":[[2022,1,3]],"date-time":"2022-01-03T05:50:22Z","timestamp":1641189022000},"page":"3316-3326","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Different platelet\u2010rich plasma preparation protocols in Female pattern hair loss: Does it affect the outcome? A pilot study"],"prefix":"10.1111","volume":"21","author":[{"given":"Nayera Hassan","family":"Moftah","sequence":"first","affiliation":[{"name":"Dermatology and Venereology Department Faculty of Medicine (for Girls) Al\u2010Azhar University  Cairo Egypt"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nour EL\u2010Eman","family":"Taha","sequence":"additional","affiliation":[{"name":"Dermatology and Venereology Department Faculty of Medicine (for Girls) Al\u2010Azhar University  Cairo Egypt"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alshaymaa Mohammed","family":"Alhabibi","sequence":"additional","affiliation":[{"name":"Clinical Pathology Department Faculty of Medicine (for Girls) Al\u2010Azhar University  Cairo Egypt"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2575-8566","authenticated-orcid":false,"given":"Mervat","family":"Hamdino","sequence":"additional","affiliation":[{"name":"Dermatology and Venereology Department Faculty of Medicine (for Girls) Al\u2010Azhar University  Cairo Egypt"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2022,1,2]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijwd.2018.08.004"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1111\/ijd.13303"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.1097\/DSS.0000000000000901"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.3390\/medicina57040311"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaad.2020.08.080"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365\u20102133"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.1155\/2013\/986970"},{"key":"e_1_2_10_9_1","first-page":"232","article-title":"A cost\u2010effective method for obtaining standard platelet\u2010rich plasma","volume":"26","author":"Kececi Y","year":"2014","journal-title":"Wounds"},{"key":"e_1_2_10_10_1","doi-asserted-by":"publisher","DOI":"10.1097\/01.EWX.0000469302.93469.65"},{"key":"e_1_2_10_11_1","doi-asserted-by":"publisher","DOI":"10.1089\/biores.2012.0225"},{"key":"e_1_2_10_12_1","doi-asserted-by":"publisher","DOI":"10.7860\/JCDR\/2015\/11011.5536"},{"key":"e_1_2_10_13_1","doi-asserted-by":"publisher","DOI":"10.1111\/bjd.12397"},{"key":"e_1_2_10_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tripleo.2008.07.016"},{"key":"e_1_2_10_15_1","doi-asserted-by":"publisher","DOI":"10.1111\/dsu.12037"},{"key":"e_1_2_10_16_1","doi-asserted-by":"publisher","DOI":"10.1097\/01.DSS.0000452629.76339.2b"},{"key":"e_1_2_10_17_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1524\u20104725.2011.02123.x"},{"key":"e_1_2_10_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaad.2020.07.006"}],"container-title":["Journal of Cosmetic Dermatology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/jocd.14648","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/jocd.14648","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/jocd.14648","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,8,20]],"date-time":"2023-08-20T03:03:04Z","timestamp":1692500584000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/jocd.14648"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,1,2]]},"references-count":17,"journal-issue":{"issue":"8","published-print":{"date-parts":[[2022,8]]}},"alternative-id":["10.1111\/jocd.14648"],"URL":"https:\/\/doi.org\/10.1111\/jocd.14648","archive":["Portico"],"relation":{},"ISSN":["1473-2130","1473-2165"],"issn-type":[{"value":"1473-2130","type":"print"},{"value":"1473-2165","type":"electronic"}],"subject":[],"published":{"date-parts":[[2022,1,2]]},"assertion":[{"value":"2021-08-10","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2021-11-22","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2022-01-02","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}