{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,21]],"date-time":"2026-06-21T03:14:33Z","timestamp":1782011673100,"version":"3.54.5"},"reference-count":15,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2022,1,8]],"date-time":"2022-01-08T00:00:00Z","timestamp":1641600000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J of Cosmetic Dermatology"],"published-print":{"date-parts":[[2022,3]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background and Objective<\/jats:title><jats:p>The use of fractional CO<jats:sub>2<\/jats:sub>\u00a0laser and pulsed dye laser (PDL) therapy to treat and\/or prevent scarring following burn injury is becoming more widespread with a number of studies reporting reduction in scar erythema and pruritus following treatment with lasers. The objective of this study was to directly compare the efficacy of PDL, fractional CO<jats:sub>2<\/jats:sub>, and PDL and fractional CO<jats:sub>2<\/jats:sub>\u00a0laser therapy in immature red hypertrophic scars in a standardized animal model.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Ten New Zealand big\u2010eared rabbits were used to make rabbit ear hypertrophic scar models. A total of 80\u00a0hypertrophic scar models were obtained and randomly divided into groups: control (Group A), Fractional CO<jats:sub>2<\/jats:sub>\u00a0laser (Group B), pulse dye laser (Group C), combined (Group D), 20 in each group. Wound sites were treated with PDL, Fractional CO<jats:sub>2<\/jats:sub>\u00a0laser, or both at 28\u00a0days post\u2010grafting. Grafts receiving no laser therapy served as controls. Hypertrophic scar appearance, morphology, size, and erythema were assessed, and punch biopsies were collected. At days 7 and 28, additional tissue was collected for biomechanical analyses and markers for HE staining, Masson staining, immunohistochemical method to determine the CD31 content in the scar; Western blot to detect the expression of VEGF protein in scars. After the day 7 and 28, the scars were collected. Histomorphological change in scars was observed by hematoxylin\u2010eosin staining, Masson staining. The expression of CD31, VEGF protein, and the cell apoptosis rate was detected by immunohistochemical method.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>(i) In morphological observation, HE staining and Masson staining, both the number of fibroblasts and amount of collagen fibrils in the experimental group were significantly reduced compared with those in control group. (ii) Micro\u2010vessel Density (MVD) value can be found much smaller in the experimental groups than the control (<jats:italic>p<\/jats:italic>\u00a0&lt;\u00a00.05). Among the four experimental groups, there was a significant difference among 14d, 21d, and 28d groups (<jats:italic>p<\/jats:italic>\u00a0&lt;\u00a00.05). (iii) On days 7 and 28 after treatment, expression of VEGF could be regulated in experimental group (<jats:italic>p<\/jats:italic>\u00a0&lt;\u00a00.05). Among the three experimental groups, the decrease of VEGF in the combined group was significantly stronger than that of the other two.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>The effect of Fractional CO<jats:sub>2<\/jats:sub>\u00a0laser combined with pulsed dye laser treatment was shown to be better than that of Fractional CO<jats:sub>2<\/jats:sub>\u00a0laser or pulsed dye laser alone and inhibits the early hypertrophic scar in rabbit ears.<\/jats:p><\/jats:sec>","DOI":"10.1111\/jocd.14732","type":"journal-article","created":{"date-parts":[[2022,1,8]],"date-time":"2022-01-08T18:20:20Z","timestamp":1641666020000},"page":"979-990","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":13,"title":["Experimental comparative study of the effect of fractional CO<sub>2<\/sub> laser combined with pulsed dye laser versus each laser alone on the treatment of hypertrophic scar of rabbit ears"],"prefix":"10.1111","volume":"21","author":[{"given":"Hong\u2010Yang","family":"Chen","sequence":"first","affiliation":[{"name":"Department of Plastic &amp; Laser Cosmetic Hunan Provincial People\u2019s Hospital  Changsha China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ying","family":"Lei","sequence":"additional","affiliation":[{"name":"Department of Plastic &amp; Laser Cosmetic Hunan Provincial People\u2019s Hospital  Changsha China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hua\u2010Wei","family":"OuYang","sequence":"additional","affiliation":[{"name":"Department of Plastic &amp; Laser Cosmetic Hunan Provincial People\u2019s Hospital  Changsha China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5183-5433","authenticated-orcid":false,"given":"Michael H.","family":"Gold","sequence":"additional","affiliation":[{"name":"Gold Skin Care Center  Nashville Tennessee USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1955-8894","authenticated-orcid":false,"given":"Jun","family":"Tan","sequence":"additional","affiliation":[{"name":"Department of Plastic &amp; Laser Cosmetic Hunan Provincial People\u2019s Hospital  Changsha China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2022,1,8]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1002\/lsm.23092"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10735-019-09828-w"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.1080\/14764172.2016.1191642"},{"key":"e_1_2_10_5_1","first-page":"54","article-title":"Comparison of the effectiveness of pulsed dye laser vs pulsed dye laser combined with ultrapulse fractional CO2 laser in the treatment of immature red hypertrophic scars","volume":"17","author":"Huawei OY","year":"2017","journal-title":"J Cosmet Dermatol"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.1002\/lsm.22304"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10103-017-2168-z"},{"key":"e_1_2_10_8_1","first-page":"41","article-title":"Safety and efficacy of a 1550nm\/1927nm dual wavelength laser for the treatment of photodamaged skin","volume":"17","author":"Narurkar VA","year":"2018","journal-title":"J Drugs Dermatol"},{"key":"e_1_2_10_9_1","first-page":"228","article-title":"Ultrapulse CO2 fractional laser intervens in situ superficial scar regeneration in a rabbit ear","volume":"17","author":"Tan J","year":"2013","journal-title":"Chin J Tissue Eng Res"},{"key":"e_1_2_10_10_1","doi-asserted-by":"publisher","DOI":"10.1111\/edt.12437"},{"key":"e_1_2_10_11_1","first-page":"176","article-title":"Red scars in hypertrophic stage with variable pulsed Nd\u2019YAG 532 nm laser: a clinical observation","volume":"18","author":"Tan J","year":"2009","journal-title":"Chin J Laser Med Surg"},{"key":"e_1_2_10_12_1","first-page":"2190","article-title":"The changes of apoptosis rate of fibroblasts and VEGF secretion after the treatment of fractional CO2 laser on hypertrophic scars model in rabbit ear","volume":"21","author":"Tan J","year":"2012","journal-title":"Chin J Aesthetic Med"},{"key":"e_1_2_10_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaad.2016.03.004"},{"key":"e_1_2_10_14_1","doi-asserted-by":"publisher","DOI":"10.1001\/jamadermatol.2013.7761"},{"key":"e_1_2_10_15_1","doi-asserted-by":"publisher","DOI":"10.1111\/jocd.13426"},{"issue":"1","key":"e_1_2_10_16_1","first-page":"22","article-title":"The current situation and prospect of laser scar treatment","volume":"26","author":"Tan J","year":"2017","journal-title":"Chin J Aesthetic Med"}],"container-title":["Journal of Cosmetic Dermatology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/jocd.14732","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/jocd.14732","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/jocd.14732","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,8,23]],"date-time":"2023-08-23T07:45:50Z","timestamp":1692776750000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/jocd.14732"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,1,8]]},"references-count":15,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2022,3]]}},"alternative-id":["10.1111\/jocd.14732"],"URL":"https:\/\/doi.org\/10.1111\/jocd.14732","archive":["Portico"],"relation":{},"ISSN":["1473-2130","1473-2165"],"issn-type":[{"value":"1473-2130","type":"print"},{"value":"1473-2165","type":"electronic"}],"subject":[],"published":{"date-parts":[[2022,1,8]]},"assertion":[{"value":"2020-11-12","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2021-12-20","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2022-01-08","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}