{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,20]],"date-time":"2026-10-20T20:27:56Z","timestamp":1792528076602,"version":"4.5.0"},"reference-count":39,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2022,1,9]],"date-time":"2022-01-09T00:00:00Z","timestamp":1641686400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100003725","name":"National Research Foundation of Korea","doi-asserted-by":"publisher","award":["2020M3E5D9080795"],"award-info":[{"award-number":["2020M3E5D9080795"]}],"id":[{"id":"10.13039\/501100003725","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J of Cosmetic Dermatology"],"published-print":{"date-parts":[[2022,9]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Collagen forms a dermal matrix in the skin. Biosynthesis and decomposition of collagen are the major processes in skin aging. Propolis is rich in flavonoids and phenolic compounds, which are known to be effective in preventing skin aging, including the enhancement of fibroblast proliferation, activation, and growth capacity.<\/jats:p><\/jats:sec><jats:sec><jats:title>Objectives<\/jats:title><jats:p>The aim of this study was to develop a poorly soluble propolis extract as an active ingredient in cosmetic products for anti\u2010aging efficacy.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods &amp; Results<\/jats:title><jats:p>Polymeric nanoparticles containing propolis extract, polyethylene glycol 400, and poloxamer 407 were prepared via a temperature\u2010induced phase transition method. The particle size of the polymeric nanoparticles was approximately 20.75\u00a0nm. The results of an <jats:italic>in vitro<\/jats:italic> procollagen type I carboxy\u2010terminal peptide assay and a matrix metalloproteinase\u20101 inhibition assay showed that the polymeric nanoparticles increased collagen production by 19.81%\u201324.59% compared to blank (<jats:italic>p<\/jats:italic>\u00a0&lt;\u00a00.05), and significantly reduced intracellular collagenase activity by 7.46%\u201331.52% compared to blank (<jats:italic>p<\/jats:italic>\u00a0&lt;\u00a00.05). In a clinical trial, polymeric nanoparticles in a cosmetic formulation were applied around the eyes of 24 female subjects for 8\u00a0weeks. Five skin parameters were significantly improved after the application of the test ampoule. Visual evaluation using the Global Photo Damage Score showed a significant reduction in wrinkles after the application of the test ampoules (<jats:italic>p<\/jats:italic>\u00a0&lt;\u00a00.001).<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>This study outlines the development of stable polymeric nanoparticles containing poorly soluble propolis in a cosmetic formulation, and its efficacy in wrinkle improvement. The developed polymeric nanoparticles were effective for alleviating wrinkles and can be used for pharmaceutical applications that utilize propolis as antiseptic, anti\u2010inflammatory, antimycotic, antifungal, antibacterial, antiulcer, anticancer, and immunomodulatory agents.<\/jats:p><\/jats:sec>","DOI":"10.1111\/jocd.14740","type":"journal-article","created":{"date-parts":[[2022,1,10]],"date-time":"2022-01-10T07:00:00Z","timestamp":1641798000000},"page":"4060-4071","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":16,"title":["Clinical Anti\u2010aging Efficacy of Propolis Polymeric Nanoparticles Prepared by a Temperature\u2010induced Phase Transition Method"],"prefix":"10.1111","volume":"21","author":[{"given":"Joo Young","family":"An","sequence":"first","affiliation":[{"name":"College of Pharmacy Korea University  Sejong\u2010ro, Sejong Republic of Korea"},{"name":"Drug Discovery Platform Research Center Korea Research Institute of Chemical Technology  Daejeon Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chaejin","family":"Kim","sequence":"additional","affiliation":[{"name":"Drug Discovery Platform Research Center Korea Research Institute of Chemical Technology  Daejeon Republic of Korea"},{"name":"College of Pharmacy Chungang University  Seoul Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Na Rae","family":"Park","sequence":"additional","affiliation":[{"name":"Drug Discovery Platform Research Center Korea Research Institute of Chemical Technology  Daejeon Republic of Korea"},{"name":"Graduate School of New Drug Discovery and Development Chungnam National University  Yusung, Daejeon Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Han Soo","family":"Jung","sequence":"additional","affiliation":[{"name":"Hanstech 1576\u201016 Chungui\u2010ro Jeongsan\u2010myeon Cheongyang\u2010gun Chungcheongnam\u2010do  Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tae\u2010sung","family":"Koo","sequence":"additional","affiliation":[{"name":"Graduate School of New Drug Discovery and Development Chungnam National University  Yusung, Daejeon Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Soon Hong","family":"Yuk","sequence":"additional","affiliation":[{"name":"College of Pharmacy Korea University  Sejong\u2010ro, Sejong Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eun Hee","family":"Lee","sequence":"additional","affiliation":[{"name":"College of Pharmacy Korea University  Sejong\u2010ro, Sejong Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sun Hang","family":"Cho","sequence":"additional","affiliation":[{"name":"Drug Discovery Platform Research Center Korea Research Institute of Chemical Technology  Daejeon Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2022,1,9]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1590\/abd1806-4841.20175481"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1034\/j.1600-0625.2002.110502.x"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.1111\/jocd.12450"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.yexcr.2010.01.020"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.jid.5700786"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.3389\/fphar.2018.00920"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms21134619"},{"key":"e_1_2_10_9_1","doi-asserted-by":"publisher","DOI":"10.3390\/molecules25030556"},{"key":"e_1_2_10_10_1","first-page":"1","article-title":"A review of the potential therapeutic and cosmetic use of propolis in topical formulations","author":"Silva MV","year":"2019","journal-title":"J. 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