{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,18]],"date-time":"2026-03-18T16:02:52Z","timestamp":1773849772641,"version":"3.50.1"},"reference-count":30,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2022,5,2]],"date-time":"2022-05-02T00:00:00Z","timestamp":1651449600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100004484","name":"Tehran University of Medical Sciences and Health Services","doi-asserted-by":"publisher","award":["93\u201003\u2010150\u201026827"],"award-info":[{"award-number":["93\u201003\u2010150\u201026827"]}],"id":[{"id":"10.13039\/501100004484","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J of Cosmetic Dermatology"],"published-print":{"date-parts":[[2022,10]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Introduction<\/jats:title><jats:p>Vitiligo is a skin disease that is associated with impaired skin immune systems and pigment degradation in skin melanocyte cells. Despite the significant impact of the disease on the quality of life of patients, treatment of the disease using an effective method such as the transplantation of uncultivated melanocytes was considered by researchers around the world. The goal of this research was to use microneedling to transplant epidermal keratinocyte\u2013melanocyte cells suspension for the treatment of vitiligo patients.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>In this study, 15\u00a0male and female vitiligo in face region patients aged 18\u201345\u00a0years were studied. In this study, melanocyte\u2013keratinocyte cells suspension was sprayed. Patients underwent microneedling treatment after spraying the cells. Before and after transplantation, patients were biometrically examined, and the quantity of pigmentation and changes in the transplanted region were documented. Statistical software was used to examine the results.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>The color difference between the lesion area and normal skin in one, two, and six months after treatment with cell suspension was significantly reduced compared with before treatment (by 48.95%). Moreover, the amount of melanin was significantly increased 6\u00a0months after treatment compared with before treatment (129.8\u00a0\u00b1\u00a04.16 vs. 195.2\u00a0\u00b1\u00a03.54, <jats:italic>p<\/jats:italic>\u00a0=\u00a00.000). A significant decrease in skin brightness in the skin of the lesion area was observed compared with normal skin, 6\u00a0months after treatment compared with before treatment (43.7\u00a0\u00b1\u00a01.44 vs. 27.9\u00a0\u00b1\u00a01.24, <jats:italic>p<\/jats:italic>\u00a0=\u00a00.000).<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Epidermal keratinocyte\u2013melanocyte cells suspension in combination with microneedling could be considered as an effective treatment of vitiligo.<\/jats:p><\/jats:sec>","DOI":"10.1111\/jocd.14987","type":"journal-article","created":{"date-parts":[[2022,4,14]],"date-time":"2022-04-14T07:42:54Z","timestamp":1649922174000},"page":"4669-4676","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":9,"title":["Combination of epidermal keratinocyte\u2013melanocyte cells suspension and microneedling: Safe surgical approach in vitiligo"],"prefix":"10.1111","volume":"21","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-7650-8076","authenticated-orcid":false,"given":"Mohammad Ali","family":"Nilforoushzadeh","sequence":"first","affiliation":[{"name":"Skin and Stem Cell Research Center Tehran University of Medical Sciences  Tehran Iran"},{"name":"Jordan Dermatology and Hair Transplantation Center  Tehran Iran"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1267-3232","authenticated-orcid":false,"given":"Maryam","family":"Nouri","sequence":"additional","affiliation":[{"name":"Skin and Stem Cell Research Center Tehran University of Medical Sciences  Tehran Iran"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shiva","family":"Alavi","sequence":"additional","affiliation":[{"name":"Skin and Stem Cell Research Center Tehran University of Medical Sciences  Tehran 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