{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,8]],"date-time":"2026-10-08T19:54:27Z","timestamp":1791489267853,"version":"4.3.4"},"reference-count":19,"publisher":"Wiley","issue":"11","license":[{"start":{"date-parts":[[2022,6,4]],"date-time":"2022-06-04T00:00:00Z","timestamp":1654300800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2022,6,4]],"date-time":"2022-06-04T00:00:00Z","timestamp":1654300800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J of Cosmetic Dermatology"],"published-print":{"date-parts":[[2022,11]]},"abstract":"Abstract<\/jats:title>\n \n Background<\/jats:title>\n We used high\u2010throughput sequencing on skin microbial flora to assess the effectiveness of an acne prescription to formulate evidence for clinical decision\u2010making.<\/jats:p>\n <\/jats:sec>\n \n Methods<\/jats:title>\n We randomized 20 outpatients into two groups. The treatment group was given the acne formula orally. The control group took capsules of the Chinese patent medicine Qingre Anchuang. Both groups used a chloramphenicol tincture externally. After 14\u2009days of treatment, we collected their skin samples and extracted the deoxyribonucleic acid for analysis.<\/jats:p>\n <\/jats:sec>\n \n Results<\/jats:title>\n \n Forty samples were sequenced in this experiment, and of these, 1865 operational taxonomic units were obtained, belonging to 736 genera and 853 strains of 34 phyla. By alpha and beta diversity analysis, the abundance of microbial species in both the experimental and control groups before treatment was higher than after treatment, indicating the intervention drugs in this experiment had a bacteriostatic effect. Through the analysis of variance, we found that\n Subdoligranulum<\/jats:italic>\n ,\n Bifidobacterium<\/jats:italic>\n ,\n Bacteroides<\/jats:italic>\n , and\n Akkermansia<\/jats:italic>\n displayed large changes during the treatment. According to the linear discriminant analysis effect size, we discovered the bacteria groups with the greatest changes in the control group after treatment were\n Firmicutes<\/jats:italic>\n ,\n Clostridia<\/jats:italic>\n ,\n Proteobacteria<\/jats:italic>\n , and\n Gammaproteobacteria.<\/jats:italic>\n The flora of the experimental group before and after treatment were\n Corynebacteriaceae<\/jats:italic>\n ,\n Corynebacteriales<\/jats:italic>\n ,\n Cutibacterium<\/jats:italic>\n ,\n Propionibacteriales<\/jats:italic>\n ,\n Propionibacteriaceae<\/jats:italic>\n , and\n Actinobacteria<\/jats:italic>\n .\n <\/jats:p>\n <\/jats:sec>\n \n Conclusion<\/jats:title>\n \n The acne prescription had a reliable intervention effect on some epidermal microbial flora of patients with acne vulgaris and could inhibit the growth of acne\u2010related microbial flora, such as\n Propionibacterium<\/jats:italic>\n .\n <\/jats:p>\n <\/jats:sec>","DOI":"10.1111\/jocd.15107","type":"journal-article","created":{"date-parts":[[2022,5,23]],"date-time":"2022-05-23T03:21:26Z","timestamp":1653276086000},"page":"6072-6078","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Exploring the clinical efficacy of a self\u2010made acne prescription based on high\u2010throughput sequencing"],"prefix":"10.1111","volume":"21","author":[{"given":"Liangyi","family":"Wang","sequence":"first","affiliation":[{"name":"Graduate School Liaoning University of Traditional Chinese Medicine Shenyang China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jingdong","family":"Wu","sequence":"additional","affiliation":[{"name":"Department of Dermatology and Cosmetology Liaoning University of Traditional Chinese Medicine Hospital 2nd Affiliated Hospital Shenyang 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