{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,8]],"date-time":"2026-10-08T19:19:47Z","timestamp":1791487187555,"version":"4.3.4"},"reference-count":34,"publisher":"Wiley","issue":"11","license":[{"start":{"date-parts":[[2022,7,19]],"date-time":"2022-07-19T00:00:00Z","timestamp":1658188800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2022,7,19]],"date-time":"2022-07-19T00:00:00Z","timestamp":1658188800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100016299","name":"Al-Ahliyya Amman University","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100016299","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J of Cosmetic Dermatology"],"published-print":{"date-parts":[[2022,11]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>Vitiligo is a common depigmentation skin disease that affects the quality of life in many patients.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Aims<\/jats:title>\n                    <jats:p>\n                      This study aims to investigate the effect of\n                      <jats:italic>Medicago sativa<\/jats:italic>\n                      methanol extract on the treatment of skin hypopigmentation disorders.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>\n                      Antioxidant activity and phytochemical constituents of the extract were determined using DDPH assay, Folin\u2013Ciocalteu, AlCl\n                      <jats:sub>3,<\/jats:sub>\n                      and HPLC\u2010MS\/MS analysis. Oil in water (o\/w) creams were prepared to contain the methanolic extract, and applied to hydroquinone\u2010induced depigmentation in vivo model and further challenged in combination with UVA light exposure. Skin and hair colors were visually scored and evaluated at different time intervals, and histopathological examinations of skin layers and hair follicles were performed.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>A total phenolic content of 187.70\u2009mg\/g, equivalent to gallic acid, and total flavonoid content of 21.97\u2009mg\/g, equivalent to quercetin, were recorded. Extract showed 71% antioxidant activity. Moreover, the HPLC\u2010MS\/MS detection revealed the presence of 18 compounds including P\u2010coumaric acid and antioxidants flavonoids, of those are seven compounds not previously detected in this species. The in vivo study showed a remarkable skin and hair pigmentation effect on plant extract\u2010treated groups, compared to the reference, placebo, and control groups. Histopathological examinations showed the growth of colored hair follicles in the dermis and epidermis layers of the extract\u2010treated mice.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>\n                      The study suggests the use of\n                      <jats:italic>M.\u00a0sativa<\/jats:italic>\n                      extract in enhancing the pigmentation process in hypopigmented skin and hair if combined with UVA light. Therefore,\n                      <jats:italic>M.\u00a0sativa<\/jats:italic>\n                      extract can be considered a potential treatment for vitiligo.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/jocd.15233","type":"journal-article","created":{"date-parts":[[2022,7,11]],"date-time":"2022-07-11T07:14:01Z","timestamp":1657523641000},"page":"6270-6280","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":9,"title":["The effect of\n                    <i>Medicago sativa<\/i>\n                    extract and light on skin hypopigmentation disorders in\n                    <scp>C57<\/scp>\n                    \/\n                    <scp>BL6<\/scp>\n                    mice"],"prefix":"10.1111","volume":"21","author":[{"given":"Azal Hussein Najm","family":"Ubaydee","sequence":"first","affiliation":[{"name":"Department of Pharmaceutical Sciences, Pharmacological and Diagnostic Research Center (PDRC), Faculty of Pharmacy Al\u2010Ahliyya Amman University  Amman Jordan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Reem","family":"Issa","sequence":"additional","affiliation":[{"name":"Department of Pharmaceutical Sciences, Pharmacological and Diagnostic Research Center (PDRC), Faculty of Pharmacy Al\u2010Ahliyya Amman University  Amman Jordan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maha N. Abu","family":"Hajleh","sequence":"additional","affiliation":[{"name":"Department of Cosmetic Science, Pharmacological and Diagnostic Research Centre, Faculty of Allied Medical Sciences Al\u2010Ahliyya Amman University  Amman Jordan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bayan\u00a0Y.","family":"Ghanim","sequence":"additional","affiliation":[{"name":"University of Petra Pharmaceutical Center (UPPC) University of Petra  Amman Jordan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Faisal","family":"Al\u2010Akayleh","sequence":"additional","affiliation":[{"name":"Department of Pharmaceutics and Pharmaceutical Technology, Faculty of Pharmacy and Medical Sciences University of Petra  Amman Jordan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9628-6036","authenticated-orcid":false,"given":"Nidal A.","family":"Qinna","sequence":"additional","affiliation":[{"name":"University of Petra Pharmaceutical 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