{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,9]],"date-time":"2026-09-09T14:35:49Z","timestamp":1788964549413,"version":"build-2803163510"},"reference-count":22,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2023,1,26]],"date-time":"2023-01-26T00:00:00Z","timestamp":1674691200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J of Cosmetic Dermatology"],"published-print":{"date-parts":[[2023,4]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>The aim of this study was to explore the anti\u2010inflammatory and anti\u2010lipid effects of lactoferrin on SZ95 human sebaceous gland cells and mouse model of acne.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>SZ95 cells were co\u2010cultured with different concentrations of lactoferrin, and cell viability was determined using the 2,5\u2010diphenyl\u20102H\u2010tetrazolium bromide method. Oil red O and Nile red staining were performed to determine the lipid content. The mRNA expression of genes related to lipid metabolism (sterol regulatory element\u2010binding protein\u20101 [<jats:italic>SREBP\u20101<\/jats:italic>], fatty acid synthase [<jats:italic>FAS<\/jats:italic>], stearoyl\u2010CoA desaturase\u20101 [<jats:italic>SCD\u20101<\/jats:italic>], fatty acid desaturase 2 [<jats:italic>FADS2<\/jats:italic>]) and inflammation (interleukin\u20108 [<jats:italic>IL\u20108<\/jats:italic>]) was determined by reverse transcription\u2013polymerase chain reaction. An acne mouse model was established using injection of <jats:italic>P<\/jats:italic>.\u00a0<jats:italic>acnes<\/jats:italic> on the backs of mice. The proliferation and apoptosis of sebaceous gland cells were examined by immunohistochemistry against proliferating cell nuclear antigen (PCNA) and TUNEL staining, respectively. Western blotting was used to detect FADS2 and CXCL15 protein expression.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Lactoferrin treatment at 10\u2013500\u2009\u03bcg\/ml significantly decreased the lipid content, as revealed by the oil red O and Nile red staining. It also attenuated the increase of mRNA expression of <jats:italic>SREBP\u20101<\/jats:italic>, <jats:italic>FAS<\/jats:italic>, <jats:italic>SCD\u20101<\/jats:italic>, <jats:italic>FADS2<\/jats:italic>, and <jats:italic>IL\u20108<\/jats:italic> in insulin\u2010treated SZ95 cells. Moreover, lactoferrin treatment at the doses of 1\u201350\u2009mg\/mouse significantly reduced the inflammation and lipid production in the mouse model of acne. Also, the number of sebaceous gland cells was significantly reduced, and apoptosis was significantly increased by lactoferrin treatment in the mice. Mechanically, the levels of FADS2 and CXCL15 proteins in tissues were significantly decreased after lactoferrin treatment in the model mice.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Our results demonstrate the potential of lactoferrin against sebogenesis, sebaceous gland inflammation in acne.<\/jats:p><\/jats:sec>","DOI":"10.1111\/jocd.15577","type":"journal-article","created":{"date-parts":[[2023,1,26]],"date-time":"2023-01-26T08:59:18Z","timestamp":1674723558000},"page":"1361-1368","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Lactoferrin regulates sebogenesis and inflammation in SZ95 human sebocytes and mouse model of acne"],"prefix":"10.1111","volume":"22","author":[{"given":"Yuan\u2010Ting","family":"Su","sequence":"first","affiliation":[{"name":"Department of Dermatology The First Affiliated Hospital of Anhui Medical University  Hefei China"},{"name":"Department of 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