{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,19]],"date-time":"2026-02-19T04:41:07Z","timestamp":1771476067944,"version":"3.50.1"},"reference-count":24,"publisher":"Wiley","issue":"11","license":[{"start":{"date-parts":[[2023,5,12]],"date-time":"2023-05-12T00:00:00Z","timestamp":1683849600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100013129","name":"Ministry of SMEs and Startups","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100013129","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J of Cosmetic Dermatology"],"published-print":{"date-parts":[[2023,11]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Purpose<\/jats:title><jats:p>Acne vulgaris is a common skin disease accompanied by chronic inflammation in the pilosebaceous follicles, resulting from excessive <jats:italic>Cutibacterium acnes<\/jats:italic>. This study aimed to investigate the inhibition of biofilm formation by <jats:italic>C. acnes<\/jats:italic> ATCC 6919 using heat\u2010treated <jats:italic>Pediococcus acidilactici<\/jats:italic> LM1013 (HT\u2010LM1013), previously isolated from the Korean traditional fermented alcoholic beverage\u2014makgeolli, and its application as a leave\u2010on\u2010type product for patients with acne vulgaris.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>HT\u2010LM1013 was prepared by Lactomason and homogenized using a high\u2010pressure homogenizer. The minimum inhibitory concentration (MIC), tricarboxylic acid (TCA) cycle, and lipase activity were evaluated for <jats:italic>C. acnes<\/jats:italic> inhibition. Inhibition of biofilm formation was demonstrated using a crystal violet solution. Damaged <jats:italic>C. acnes<\/jats:italic> was observed using field\u2010emission scanning electron microscopy (FE\u2010SEM). Clinical trials were performed using a leave\u2010on\u2010type product containing HT\u2010LM1013.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>HT\u2010LM1013 inhibited the TCA cycle (36.80%) and lipase activity using palmitate (31.89%), stearate (36.91%), and oleate (30.86%) as substrates at 1\u2009\u00d7\u2009MIC (<jats:italic>p<\/jats:italic>\u2009&lt;\u20090.01). After treatment with HT\u2010LM1013, concave and elongated shapes of <jats:italic>C. acnes<\/jats:italic> were observed by FE\u2010SEM. In addition, HT\u2010LM1013 inhibited biofilm formation by 71.75% at 1\u2009\u00d7\u2009MIC (<jats:italic>p<\/jats:italic>\u2009&lt;\u20090.001) and removed 73.35% of mature biofilms (<jats:italic>p<\/jats:italic>\u2009&lt;\u20090.01). In the clinical trial, the leave\u2010on\u2010type product decreased the number of closed comedones from 14.04 to 10.22, open comedones from 7.22 to 4.39%, and sebum content to 76.23% at week 4 (<jats:italic>p<\/jats:italic>\u2009&lt;\u20090.01). The satisfaction score of the participants was recorded 3.83 on a five\u2010point scale.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>HT\u2010LM1013 is potent for the treatment of acne vulgaris.<\/jats:p><\/jats:sec>","DOI":"10.1111\/jocd.15809","type":"journal-article","created":{"date-parts":[[2023,5,13]],"date-time":"2023-05-13T06:40:09Z","timestamp":1683960009000},"page":"3125-3134","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":10,"title":["Heat\u2010treated <i>Pediococcus acidilactici<\/i> LM1013\u2010mediated inhibition of biofilm formation by <i>Cutibacterium acnes<\/i> and its application in acne vulgaris: A single\u2010arm clinical trial"],"prefix":"10.1111","volume":"22","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-6615-2547","authenticated-orcid":false,"given":"Won\u2010Young","family":"Bae","sequence":"first","affiliation":[{"name":"Microbiome R&amp;D Center Lactomason  Seoul Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2474-1973","authenticated-orcid":false,"given":"Woo\u2010Hyun","family":"Jung","sequence":"additional","affiliation":[{"name":"Microbiome R&amp;D Center Lactomason  Seoul Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2456-1673","authenticated-orcid":false,"given":"Young Jin","family":"Lee","sequence":"additional","affiliation":[{"name":"Microbiome R&amp;D Center Lactomason  Seoul Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1683-4638","authenticated-orcid":false,"given":"So Lim","family":"Shin","sequence":"additional","affiliation":[{"name":"Microbiome R&amp;D Center Lactomason  Seoul Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9636-8189","authenticated-orcid":false,"given":"Yu\u2010Kyung","family":"An","sequence":"additional","affiliation":[{"name":"Microbiome R&amp;D Center Lactomason  Seoul Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8066-1161","authenticated-orcid":false,"given":"Tae\u2010Rahk","family":"Kim","sequence":"additional","affiliation":[{"name":"Microbiome R&amp;D Center Lactomason  Seoul Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6278-1795","authenticated-orcid":false,"given":"Minn","family":"Sohn","sequence":"additional","affiliation":[{"name":"Microbiome R&amp;D Center Lactomason  Seoul 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