{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2024,5,29]],"date-time":"2024-05-29T19:49:56Z","timestamp":1717012196059},"reference-count":47,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2023,7,18]],"date-time":"2023-07-18T00:00:00Z","timestamp":1689638400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["#81960249"],"award-info":[{"award-number":["#81960249"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J of Cosmetic Dermatology"],"published-print":{"date-parts":[[2024,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Adipose stem cell\u2010derived exosomes (ADSC\u2010EXO) and botulinum toxin type A (BTX\u2010A) individually showed a therapeutic effect on skin wound repair.<\/jats:p><\/jats:sec><jats:sec><jats:title>Aims<\/jats:title><jats:p>This study investigated their synergistic effect on promoting skin wound healing in vitro and in vivo and the underlying molecular events.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>ADSCs were isolated from Sprague\u2013Dawley (SD) rats to obtain ADSC\u2010EXO by ultrafiltration and ultracentrifugation and were confirmed using nanoparticle tracking analysis and transmission electron microscopy. Human skin fibroblasts (HSF) were cultured and treated with or without ADSC\u2010EXO, BTX\u2010A, or their combination. Changes in cell phenotypes and protein expression were analyzed using different in vitro assays, and a rat skin wound model was used to assess their in vivo effects.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>The isolated ADSC\u2010EXO from primarily cultured ADSCs had a circular vesicle shape with a 30\u2013180\u2009nm diameter. Treatment of HSF with ADSC\u2010EXO and\/or BTX\u2010A significantly accelerated HSF migration in vitro and skin wound healing in a rat model. Moreover, ADSC\u2010EXO plus BTX\u2010A treatment dramatically induced VEGFA expression but reduced COL III and COL I levels in vivo. ADSC\u2010EXO and\/or BTX\u2010A treatment significantly upregulated TGF\u2010\u03b23 expression on Day 16 after surgery but downregulated TGF\u2010\u03b21 expression, suggesting that ADSC\u2010EXO plus BTX\u2010A promoted skin wound healing and reduced inflammatory cell infiltration.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>The ADSC\u2010EXO plus BTX\u2010A treatment demonstrated a synergistic effect on skin wound healing through upregulation of VEGF expression and the TGF\u2010\u03b23\/TGF\u2010\u03b21 and COL III\/COL I ratio.<\/jats:p><\/jats:sec>","DOI":"10.1111\/jocd.15922","type":"journal-article","created":{"date-parts":[[2023,7,19]],"date-time":"2023-07-19T05:30:57Z","timestamp":1689744657000},"page":"271-283","update-policy":"http:\/\/dx.doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Experimental study on the effect and mechanism of adipose stem cell\u2010derived exosomes combined with botulinum 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