{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,9]],"date-time":"2026-06-09T15:51:35Z","timestamp":1781020295715,"version":"3.54.1"},"reference-count":44,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2023,8,30]],"date-time":"2023-08-30T00:00:00Z","timestamp":1693353600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J of Cosmetic Dermatology"],"published-print":{"date-parts":[[2024,2]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>In traditional Asian medicine, <jats:italic>Gynostemma pentaphyllum<\/jats:italic> Makino leaf extract (Gp) is used to treat aging, metabolic syndrome, diabetes, and neurodegenerative diseases. Hair loss and hair\u2010graying are common phenomena that haunt everyone. However, whether Gp activities on inhibition of hair loss and getting gray have been rarely studied.<\/jats:p><\/jats:sec><jats:sec><jats:title>Aim<\/jats:title><jats:p>Study the Gp activity and mechanism by in vivo and in vitro experiments to explore its application on hair health.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>In the present study, we determined the effects of Gp on the expression of hair growth\u2010related genes and proliferation of human dermal papilla cells (hDPCs). Furthermore, Gp was topically applied to the hair\u2010shaved skin of male C57BL\/6 mice, and the histological profile of the skin was studied. Because emotional stress may lead to melanocyte disappearance, norepinephrine\u2010exposed mice B16 melanocytes were treated with Gp to elucidate the anti\u2010hair graying capacity of Gp in response to this stress type.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Gp stimulated the proliferation of hDPCs and the Wnt signaling pathways associated with hair growth; furthermore, the expression of the hair loss\u2010related gene transforming growth factor\u2010\u03b21 was suppressed. Gp treatment significantly increased the size of hair follicles in the treated mice and stimulated them. Moreover, Gp not only increased melanin synthesis but also tyrosinase activity in B16 cells. Quantitative real\u2010time polymerase chain reaction revealed that Gp increased melanin synthesis by increasing the expression of tyrosine\u2010related protein\u20101, tyrosine\u2010related protein\u20102, tyrosinase, and microphthalmia\u2010associated transcription factor.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Our study provides preclinical evidence regarding the potential of Gp as a promising hair growth and anti\u2010graying agent.<\/jats:p><\/jats:sec>","DOI":"10.1111\/jocd.15963","type":"journal-article","created":{"date-parts":[[2023,8,31]],"date-time":"2023-08-31T05:42:49Z","timestamp":1693460569000},"page":"648-657","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["<i>Gynostemma pentaphyllum<\/i> Makino extract induces hair growth and exhibits an anti\u2010graying effect via multiple 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