{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,2]],"date-time":"2026-06-02T09:40:15Z","timestamp":1780393215272,"version":"3.54.1"},"reference-count":25,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2023,11,20]],"date-time":"2023-11-20T00:00:00Z","timestamp":1700438400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J of Cosmetic Dermatology"],"published-print":{"date-parts":[[2024,2]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>In recent years, aesthetic procedures aiming at body remodeling and have grown exponentially. Cryolipolysis (CLL) has stood out as a noninvasive resource that acts directly on the subcutaneous adipose tissue promoting a significant reduction of adipose tissue through of cooling that could lead to the crystallization cytoplasmic lipids, loss of cellular integrity, apoptosis\/necrosis of adipocytes, and local inflammation, producing selective loss of adipose tissue. Thus, the objective of the present study was to evaluate the effects of a specific technique of CLL application on the inflammatory reactions of the target tissue in different post\u2010application times.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>This is a randomized, blind clinical study that evaluated the tissue sample of six patients after 45, 60, and 90\u2009days of an innovative protocol for the application of CLL, with samples collected through abdominoplasty surgeries. The samples were evaluated by immunohistochemical analyses of several markers.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>A significantly greater increase in fibroblasts was observed at 45\u2009days and greater phagocytic action at 60\u2009days.<\/jats:p><jats:p>Regarding the apoptosis process, the expression of caspase 3 and cleaved caspase 3 markers varied at different times, with cleaved caspase 3 being higher at 45 and 90\u2009days after CLL application.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>The protocol of the CLL presented in this study was able to induce inflammatory responses in addition to confirming the selective apoptotic action at the different times studied.<\/jats:p><\/jats:sec>","DOI":"10.1111\/jocd.16002","type":"journal-article","created":{"date-parts":[[2023,11,21]],"date-time":"2023-11-21T22:17:31Z","timestamp":1700605051000},"page":"441-449","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Innovation of the protocol for the application of cryolipolysis: Effects and mechanisms of action"],"prefix":"10.1111","volume":"23","author":[{"given":"Christiane Rodrigues Tofoli","family":"Palauro","sequence":"first","affiliation":[{"name":"Centro Universit\u00e1rio de Vila Velha  Vila Velha Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8530-8183","authenticated-orcid":false,"given":"Patr\u00edcia Froes","family":"Meyer","sequence":"additional","affiliation":[{"name":"International Research Group  Natal Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6861-6640","authenticated-orcid":false,"given":"Ciro Dantas","family":"Soares","sequence":"additional","affiliation":[{"name":"International Research Group  Natal Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eneida","family":"de Morais\u00a0Carreiro","sequence":"additional","affiliation":[{"name":"International Research Group  Natal Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fl\u00e1vio","family":"de Paiva\u00a0Dumaresq","sequence":"additional","affiliation":[{"name":"Universidade Estadual de Ci\u00eancias da Sa\u00fade de Alagoas  Maceio Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fernando Cesar Camara","family":"de Oliveira","sequence":"additional","affiliation":[{"name":"Universidade Federal do Rio Grande do Norte  Natal Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1137-7180","authenticated-orcid":false,"given":"Ana Laura Martins","family":"de Andrade","sequence":"additional","affiliation":[{"name":"Universidade Brasil  Sao Paulo Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Priscila","family":"da S\u00a0P\u00a0S\u00a0Daumas","sequence":"additional","affiliation":[{"name":"Universidade Est\u00e1cio de S\u00e1  Rio de Janeiro Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2023,11,20]]},"reference":[{"key":"e_1_2_11_2_1","doi-asserted-by":"publisher","DOI":"10.1097\/DSS.0000000000002420"},{"issue":"4","key":"e_1_2_11_3_1","first-page":"1","article-title":"Statistics 2018","volume":"39","author":"The American Society for Aesthetic Plastic Surgery's Cosmetic Surgery National Data Bank","year":"2019","journal-title":"Aesthetic Surg J"},{"issue":"2","key":"e_1_2_11_4_1","first-page":"1","article-title":"Cryolipolysis: clinical best practices and other nonclinical considerations","volume":"2","author":"Few J","year":"2020","journal-title":"Aesthetic Surg J"},{"key":"e_1_2_11_5_1","doi-asserted-by":"publisher","DOI":"10.1097\/PRS.0000000000001236"},{"key":"e_1_2_11_6_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM197004232821709"},{"key":"e_1_2_11_7_1","doi-asserted-by":"publisher","DOI":"10.1002\/lsm.20719"},{"key":"e_1_2_11_8_1","unstructured":"US Food and Drug Administration2023. 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