{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,30]],"date-time":"2026-05-30T03:36:36Z","timestamp":1780112196069,"version":"3.54.0"},"reference-count":50,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2023,10,31]],"date-time":"2023-10-31T00:00:00Z","timestamp":1698710400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2023,10,31]],"date-time":"2023-10-31T00:00:00Z","timestamp":1698710400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J of Cosmetic Dermatology"],"published-print":{"date-parts":[[2024,3]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>Hair loss occurs due to various biological and environmental causes, which can have psychosocial consequences. The Wnt\/\u03b2\u2010catenin signaling is well\u2010known for its role in hair growth and regeneration, as it induces the proliferation and differentiation of hair cells. When the leucine\u2010rich G protein\u2010coupled receptor 5 (Lgr5) interacts with the R\u2010spondins, the frizzled receptor (FZD), a Wnt receptor, becomes stabilized, resulting in an increased \u03b2\u2010catenin activity.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Aim<\/jats:title>\n                    <jats:p>We investigated whether the octapeptide that binds to Lgr5 enhances proliferation and differentiation of human primary hair cells through the activation of Wnt\/\u03b2\u2010catenin signaling.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>The binding affinity of the octapeptide to Lgr5 was evaluated using surface plasmon resonance (SPR). We confirmed changes in proliferation and related factors like \u03b2\u2010catenin activation and growth factors (GFs) expression in human hair follicle dermal papilla cells (HHFDPCs). Additionally, we observed the proliferation and the expression of differentiation markers in human hair follicle outer root sheath cells (HHFORSCs), human hair follicle germinal matrix cells (HHFGMCs), and human hair follicle stem cells (HHFSCs). We used three\u2010dimensional HHFDPC spheroid culture treated with dihydrotestosterone (DHT) to create in\u00a0vitro conditions that mimic androgenetic alopecia, and we studied the effects of octapeptide on Wnt expression and HHFSC differentiation.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>The binding of the octapeptide to Lgr5 was confirmed using SPR analysis. In HHFDPCs, treatment with octapeptide resulted in a concentration\u2010dependent increase in proliferation. We also observed increased nuclear translocation of \u03b2\u2010catenin and increased expression of its downstream targets. HHFDPCs treated with octapeptide exhibited increased expression of growth factors and phosphorylation of Akt and ERK. In addition, we confirmed that octapeptide increased proliferation and induced differentiation in HHFORSCs, HHFGMCs, and HHFSCs. Under the HHFDPC spheroid culture conditions, we found that octapeptide restored the inhibition of Wnt\u20105a and Wnt\u201010b expressions by DHT. In HHFSCs treated with HHFDPC spheroid culture media, we observed that octapeptide recovered the inhibition of differentiation by DHT.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>We found that octapeptides activated the Wnt\/\u03b2\u2010catenin signaling and induced the proliferation and differentiation of human primary hair cells by acting as an exogenous ligand for Lgr5. In addition, octapeptides recovered inhibited hair regeneration characters by DHT in androgenetic alopecia\u2010mimic in\u00a0vitro model. These findings suggest that octapeptides may be a promising therapeutic option for treating hair loss.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/jocd.16036","type":"journal-article","created":{"date-parts":[[2023,10,31]],"date-time":"2023-10-31T03:53:55Z","timestamp":1698724435000},"page":"986-998","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["In vitro hair growth\u2010promoting effect of Lgr5\u2010binding octapeptide in human primary hair cells"],"prefix":"10.1111","volume":"23","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-9083-7298","authenticated-orcid":false,"given":"Eung Ji","family":"Lee","sequence":"first","affiliation":[{"name":"Caregen R&amp;D center  Anyang\u2010si Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Min Woong","family":"Kim","sequence":"additional","affiliation":[{"name":"Caregen R&amp;D center  Anyang\u2010si Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ha\u2010Na","family":"Gil","sequence":"additional","affiliation":[{"name":"Caregen R&amp;D center  Anyang\u2010si Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yong Ji","family":"Chung","sequence":"additional","affiliation":[{"name":"Caregen R&amp;D center  Anyang\u2010si Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3543-7766","authenticated-orcid":false,"given":"Eun Mi","family":"Kim","sequence":"additional","affiliation":[{"name":"Caregen R&amp;D center  Anyang\u2010si 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