{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,24]],"date-time":"2026-04-24T18:20:34Z","timestamp":1777054834345,"version":"3.51.4"},"reference-count":46,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2024,3,27]],"date-time":"2024-03-27T00:00:00Z","timestamp":1711497600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J of Cosmetic Dermatology"],"published-print":{"date-parts":[[2024,6]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Objectives<\/jats:title><jats:p><jats:italic>Malassezia<\/jats:italic> species are common, clinically relevant, and lipid\u2010dependent yeasts of humans. They are also the leading causes of the dandruff problem of humans, and the azoles are used primarily in their topical and systemic treatment. Resistance to azoles is an emerging problem among <jats:italic>Malassezia<\/jats:italic> sp., which indicates the need of new drug assessments that will be effective against dandruff and limit the use of azoles and other agents in treatment. Among them, the efficacy of various combinations of piroctone olamine and climbazole against <jats:italic>Malassezia<\/jats:italic> sp. is\u00a0highly important. Here, we assessed the efficacies of various piroctone olamine and climbazole formulations against <jats:italic>Malassezia<\/jats:italic> sp. in comparison with ketoconazole.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>A total of nine formulations were included in the study, where each formulation was prepared from different concentrations of piroctone olamine and climbazole and both. All formulations contained the same ingredients as water, surfactants, hair conditioning agents, and preservatives. <jats:italic>Malassezia<\/jats:italic> furfur CBS1878, <jats:italic>Malassezia globosa<\/jats:italic> CBS7874, and <jats:italic>Malassezia sympodialis<\/jats:italic> CBS9570 were tested for antifungal susceptibility of each formulation by agar diffusion method. Sizes of the inhibition zones were compared with standard medical shampoo containing 2% ketoconazole, and the data were analyzed by Dunnett's multiple\u2010comparison test.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>For all <jats:italic>Malassezia<\/jats:italic> sp. strains, climbazole 0.5% and piroctone olamine\/climbazole (0.1%\/0.1% and 0.1%\/0.5%) combinations were found to have the same effect as the medical shampoo containing 2% ketoconazole. Piroctone olamine\/climbazole 1.0%\/0.1% formulation showed the same efficacy as 2% ketoconazole on <jats:italic>M<\/jats:italic>.\u2009<jats:italic>furfur<\/jats:italic> and <jats:italic>M<\/jats:italic>.\u2009<jats:italic>sympodialis<\/jats:italic>, while 0.1%\/0.5% formulation to only <jats:italic>M<\/jats:italic>.\u2009<jats:italic>furfur<\/jats:italic>. For <jats:italic>M<\/jats:italic>.\u2009<jats:italic>globosa<\/jats:italic>, none of the formulations tested were as effective as ketoconazole.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>The species distribution of <jats:italic>Malassezia<\/jats:italic> sp. varies depending on the anatomical location on the host. According to the results of this study, climbazole and piroctone olamine combinations seem to be promising options against the dandruff problem with their high antifungal\/anti dandruff efficacy.<\/jats:p><\/jats:sec>","DOI":"10.1111\/jocd.16219","type":"journal-article","created":{"date-parts":[[2024,3,28]],"date-time":"2024-03-28T05:46:37Z","timestamp":1711604797000},"page":"2078-2083","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Evaluation of novel cosmetic shampoo formulations against <i>Malassezia<\/i> species: Preliminary results of <scp>anti\u2010dandruff<\/scp> shampoo formulations"],"prefix":"10.1111","volume":"23","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-7783-8723","authenticated-orcid":false,"given":"\u00c7a\u011fr\u0131","family":"Ergin","sequence":"first","affiliation":[{"name":"Department of Medical Microbiology Pamukkale University School of Medicine  Denizli 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