{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,6]],"date-time":"2026-03-06T04:35:21Z","timestamp":1772771721654,"version":"3.50.1"},"reference-count":24,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2024,3,1]],"date-time":"2024-03-01T00:00:00Z","timestamp":1709251200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["82202018"],"award-info":[{"award-number":["82202018"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J of Cosmetic Dermatology"],"published-print":{"date-parts":[[2024,6]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Concentrated growth factor (CGF) injection has proven effective in treating androgenetic alopecia (AGA). The primary mechanism of CGF in treating AGA is thought to be the CD34+ stem cells and platelets\u2010associated growth factors being injected into the scalp. CGF efficacy in treating AGA may rely on the activation level of these stem cells and platelets. The 640\u2009nm laser is a United States Food and Drug Administration approved AGA treatment that activates follicle stem cells. Therefore, we hypothesize that pretreating CGF with a 640\u2009nm laser may further activate CD34+ stem cells and platelets, thereby improving the efficacy of CGF in treating AGA.<\/jats:p><\/jats:sec><jats:sec><jats:title>Objective<\/jats:title><jats:p>This study aims to investigate whether 640\u2009nm laser pretreated CGF (640CGF) has a greater effect in treating AGA than 640\u2009nm laser non\u2010pretreated CGF (N640CGF) and evaluate whether 640\u2009nm laser pretreatment changed CD34+ cell percentage.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>This study enrolled 10 patients (8\u2009male, 2\u2009female) with AGA aged 18\u201360\u2009years who received CGF injections. The 640CGF group was pretreated with a 640\u2009nm laser at an energy density of 4\u2009J\/cm<jats:sup>2<\/jats:sup>, with a 30\u2009cm irradiation distance for 30\u2009min. Half of the scalp was treated with 640CGF, whereas the other half was treated with N640CGF. The injection was prepared by a doctor who did not know which blood tube had been pretreated. The treatment efficacy was evaluated using a trichoscope 1\u2009month after injection.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>All 10 (100%) patients participated in the follow\u2010up visit, and a higher quantity of new hairs was observed on the side injected with 640CGF than N640CGF (<jats:italic>p<\/jats:italic>\u2009=\u20090.019). Additionally, fewer malnourished hairs were observed on the 640CGF pretreated side (<jats:italic>p<\/jats:italic>\u2009=\u20090.015). No serious adverse events were reported.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>A higher percentage of CD34+ stem cells and improved\u2002efficacy\u2002in AGA treatment could be observed with CGF prepared from 640\u2009nm laser\u2010pretreated blood.<\/jats:p><\/jats:sec>","DOI":"10.1111\/jocd.16249","type":"journal-article","created":{"date-parts":[[2024,3,2]],"date-time":"2024-03-02T05:53:41Z","timestamp":1709358821000},"page":"2249-2255","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Higher percentage of <scp>CD34<\/scp>+ stem cells and elevated efficacy in androgenetic alopecia treatment observed in <scp>CGF<\/scp> prepared from 640\u2009nm laser\u2010pretreated blood: A preliminary study"],"prefix":"10.1111","volume":"23","author":[{"given":"Jin","family":"Gao","sequence":"first","affiliation":[{"name":"Department of Dermatology, Shanghai East Hospital Tongji University School of Medicine  Shanghai China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Qin","family":"Xiao","sequence":"additional","affiliation":[{"name":"Department of Dermatology, Shanghai East Hospital Tongji University School of Medicine  Shanghai China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0649-4545","authenticated-orcid":false,"given":"Yongzhou","family":"Lu","sequence":"additional","affiliation":[{"name":"Department of Dermatology, Shanghai East Hospital Tongji University School of Medicine  Shanghai China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jing","family":"Guo","sequence":"additional","affiliation":[{"name":"Department of Dermatology, Shanghai East Hospital Tongji University School of Medicine  Shanghai China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dongdong","family":"Luan","sequence":"additional","affiliation":[{"name":"Department of Dermatology, Shanghai East Hospital Tongji University School of Medicine  Shanghai China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chuanlong","family":"Jia","sequence":"additional","affiliation":[{"name":"Department of Dermatology, Shanghai East Hospital Tongji University School of Medicine  Shanghai China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0707-7441","authenticated-orcid":false,"given":"Qiannan","family":"Xu","sequence":"additional","affiliation":[{"name":"Department of Dermatology, Shanghai East Hospital Tongji University School of Medicine  Shanghai China"},{"name":"Department of Dermatology, Shanghai Ruijin Hospital Jiaotong University School of Medicine  Shanghai China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nan","family":"Xu","sequence":"additional","affiliation":[{"name":"Department of Dermatology, Shanghai East Hospital Tongji University School of Medicine  Shanghai 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