{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,22]],"date-time":"2026-10-22T14:21:36Z","timestamp":1792678896801,"version":"4.5.0"},"reference-count":50,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2024,5,20]],"date-time":"2024-05-20T00:00:00Z","timestamp":1716163200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2024,5,20]],"date-time":"2024-05-20T00:00:00Z","timestamp":1716163200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J of Cosmetic Dermatology"],"published-print":{"date-parts":[[2024,9]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>Extracellular vesicles (EVs) derived from mesenchymal stem cells (MSCs) are effective in the treatment of skin photoaging; however, their low yield and functional decline with passage progression limit their clinical application. Cell\u2010derived nanovesicles (CNVs) are potential alternatives that can address the limitations of EVs derived from MSCs and are conducive to clinical transformations. Hair follicle mesenchymal stem cells (HFMSCs), a type of MSCs, have demonstrated the function of repairing skin tissues; nevertheless, the efficacy of CNVs from HFMSCs (HFMSC\u2010CNVs) in the treatment of skin photoaging remains unclear. Therefore, ultraviolet radiation B (UVB)\u2010induced photoaging nude mice and human dermal fibroblasts (HDFs) were used as experimental models to investigate the therapeutic effects of HFMSC\u2013CNVs in photoaging models.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>HFMSC\u2013CNVs were successfully prepared using the mechanical extrusion method. UVB\u2010induced nude mice and HDFs were used as experimental models of photoaging. Multiple approaches, including hematoxylin\u2013eosin and Masson staining, immunohistochemistry, immunofluorescence, detection of reactive oxygen species (ROS), flow cytometry, western blotting, and other experimental methods, were combined to investigate the possible effects and mechanisms of HFMSC\u2013CNVs in the treatment of skin photoaging.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      In the nude mouse model of skin photoaging, treatment with HFMSC\u2013CNVs reduced UVB\u2010induced skin wrinkles (\n                      <jats:italic>p<\/jats:italic>\n                      \u2009&lt;\u20090.05) and subcutaneous capillary dilation, alleviated epidermis thickening (\n                      <jats:italic>p<\/jats:italic>\n                      \u2009&lt;\u20090.001), and dermal thinning (\n                      <jats:italic>p<\/jats:italic>\n                      \u2009&lt;\u20090.001). Furthermore, HFMSC\u2013CNVs upregulated proliferating cell nuclear antigen (PCNA) expression (\n                      <jats:italic>p<\/jats:italic>\n                      \u2009&lt;\u20090.05) and decreased the levels of ROS,\n                      <jats:italic>\u03b2\u2010galactosidase<\/jats:italic>\n                      (\u03b2\u2010Gal), and CD86 (\n                      <jats:italic>p<\/jats:italic>\n                      \u2009&lt;\u20090.01). In\u00a0vitro experiments, treatment with HFMSC\u2013CNVs enhanced the cellular activity of UVB\u2010exposed HDFs (\n                      <jats:italic>p<\/jats:italic>\n                      \u2009&lt;\u20090.05), and reduced ROS levels and the percentage of senescent cells (\n                      <jats:italic>p<\/jats:italic>\n                      \u2009&lt;\u20090.001), and alleviated cell cycle arrest (\n                      <jats:italic>p<\/jats:italic>\n                      \u2009&lt;\u20090.001). HFMSC\u2013CNVs upregulated the expression of Collagen I (Col I), SMAD2\/3, transforming growth factor beta (TGF\u2010\u03b2),\n                      <jats:italic>catalase<\/jats:italic>\n                      (CAT), glutathione peroxidase\u20101 (GPX\u20101), and superoxide dismutase\u20101 (SOD\u20101) (\n                      <jats:italic>p<\/jats:italic>\n                      \u2009&lt;\u20090.05) and downregulated the expression of\n                      <jats:italic>cycle suppressor protein<\/jats:italic>\n                      (p53), cell cycle suppressor protein (p21), and matrix metalloproteinase 3 (MMP3) (\n                      <jats:italic>p<\/jats:italic>\n                      \u2009&lt;\u20090.05).\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>Conclusively, the anti\u2010photoaging properties of HFMSC\u2013CNVs were confirmed both in\u00a0vivo and in\u00a0vitro. HFMSC\u2013CNVs exert anti\u2010photoaging effects by alleviating cell cycle arrest, decreasing cellular senescence and macrophage infiltration, promoting cell proliferation and extracellular matrix (ECM) production, and reducing oxidative stress by increasing the activity of antioxidant enzymes.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/jocd.16336","type":"journal-article","created":{"date-parts":[[2024,5,21]],"date-time":"2024-05-21T02:54:51Z","timestamp":1716260091000},"page":"3005-3020","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":12,"title":["The role and mechanism of engineered nanovesicles derived from hair follicle mesenchymal stem cells in the treatment of\n                    <scp>UVB<\/scp>\n                    \u2010induced skin photoaging"],"prefix":"10.1111","volume":"23","author":[{"given":"Zhounan","family":"Jiang","sequence":"first","affiliation":[{"name":"Affiliated Hangzhou First People's Hospital, School Of Medicine, Westlake University  Hangzhou China"},{"name":"The Second Affiliated Hospital Zhejiang University School Of Medicine  Hangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hanxiao","family":"Cheng","sequence":"additional","affiliation":[{"name":"Affiliated Hangzhou First People's Hospital, School Of Medicine, Westlake University  Hangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xifei","family":"Qian","sequence":"additional","affiliation":[{"name":"The Fourth School of Clinical Medicine Zhejiang Chinese Medical University  Hangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jingyi","family":"Tu","sequence":"additional","affiliation":[{"name":"The Fourth School of Clinical Medicine Zhejiang Chinese Medical University  Hangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chongxiang","family":"Fan","sequence":"additional","affiliation":[{"name":"The Fourth School of Clinical Medicine Zhejiang Chinese Medical University  Hangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yirui","family":"Pan","sequence":"additional","affiliation":[{"name":"Affiliated Hangzhou First People's Hospital, School Of Medicine, Westlake University  Hangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhiwei","family":"Lin","sequence":"additional","affiliation":[{"name":"Zhejiang Healthfuture Biomedicine Co., Ltd.  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