{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,16]],"date-time":"2026-10-16T20:54:43Z","timestamp":1792184083637,"version":"4.4.0"},"reference-count":48,"publisher":"Wiley","issue":"S5","license":[{"start":{"date-parts":[[2024,11,28]],"date-time":"2024-11-28T00:00:00Z","timestamp":1732752000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J of Cosmetic Dermatology"],"published-print":{"date-parts":[[2024,12]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Hair loss is linked to dysfunction of the growth (anagen), regression (catagen) and rest (telogen) phases of the hair follicle (HF) cycle.<\/jats:p><\/jats:sec><jats:sec><jats:title>Aims<\/jats:title><jats:p>To evaluate the effects of a <jats:italic>Silybum marianum<\/jats:italic> extract (SME), manganese PCA (MnPCA), and a <jats:italic>Lespedeza capitata<\/jats:italic> extract (LCE) on markers of hair growth and anchorage in human follicle dermal papilla cells (HFDPCs), and to investigate the ability of a topical serum containing these active ingredients to improve HF growth in an <jats:italic>ex vivo<\/jats:italic> human scalp skin model.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>In HFDPCs, we assessed receptor tyrosine kinase phosphorylation and Wnt\/\u03b2\u2010catenin pathway activation; quantified versican, vascular endothelial growth factor (VEGF) and Dickkopf\u20101 (DDK1) secretion; and evaluated 5\u03b1\u2010reductase (5\u03b1R) activity. Using scalp skin biopsies from two female donors, we measured hair shaft elongation, analyzed hair matrix keratinocyte proliferation and apoptosis, and determined HF cycle stage and score.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Compared to untreated HFDPCs, SME upregulated phosphorylation of growth factor receptors (EGFR:1.9\u2009\u00d7\u2009and PDGFR: 2.8\u2009\u00d7) and their downstream effectors (ERK, GSK3, Akt, and STAT: 1.2\u20132.0\u2009\u00d7); MnPCA enhanced versican (33.0\u2009\u00d7) and VEGF (3.3\u2009\u00d7) secretion, and stimulated the Wnt\/\u03b2\u2010catenin pathway (+80%); and LCE reduced DKK1 secretion (\u221272%) and 5\u03b1R activity (dihydrotestosterone\/testosterone ratio: \u221260%). Compared to untreated scalp skin biopsies, the serum enhanced hair shaft elongation (+102%), and significantly prolonged the anagen phase by improving hair cycle scores and stimulating hair matrix keratinocyte proliferation (+58%).<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>SME, MnPCA, and LCE displayed complementary anti\u2010hair loss properties. The serum combining these active ingredients may be useful in hair loss treatment.<\/jats:p><\/jats:sec>","DOI":"10.1111\/jocd.16616","type":"journal-article","created":{"date-parts":[[2024,11,28]],"date-time":"2024-11-28T11:05:42Z","timestamp":1732791942000},"page":"1-11","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["New Plant Extracts Exert Complementary Anti\u2010Hair Loss Properties in Human In\u00a0Vitro and Ex\u00a0Vivo Models"],"prefix":"10.1111","volume":"23","author":[{"given":"Daniel","family":"Bacqueville","sequence":"first","affiliation":[{"name":"R &amp; D Department Pierre Fabre Dermo\u2010Cosm\u00e9tique and Personal Care  Toulouse France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marguerite","family":"L\u00e9v\u00eaque","sequence":"additional","affiliation":[{"name":"R &amp; D Department Pierre Fabre Dermo\u2010Cosm\u00e9tique and Personal Care  Toulouse 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