{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,27]],"date-time":"2026-03-27T09:04:07Z","timestamp":1774602247325,"version":"3.50.1"},"reference-count":41,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2025,2,27]],"date-time":"2025-02-27T00:00:00Z","timestamp":1740614400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J of Cosmetic Dermatology"],"published-print":{"date-parts":[[2025,3]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:sec><jats:title>Objective<\/jats:title><jats:p>Dry and chapped lips adversely affect both lip structure and function, yet there is no established gold standard for their treatment. Herbal extracts present a promising alternative due to their natural properties, though their therapeutic potential for lip care remains underexplored. This study aims to develop and clinically evaluate a novel lip hydrant formulated with polyherbal extracts, with the objective of offering an effective solution for managing dry and chapped lips.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Six herbal extracts and eight mixtures, consisting of at least three herbal extracts, were evaluated for cytotoxicity using the MTT assay in HaCaT cells. The essential molecular markers were examined by western blot analysis and immunofluorescence assay. The selected mixture was formulated into lip hydrant and tested in a randomized, double\u2010blind, controlled clinical trial. The 66 Thai participants with dry lip concerns were randomly assigned into two groups. Each participant applied either the lip hydrant (test group) or petroleum gel (control group) once daily for 28\u2009days. Clinical assessments were performed at baseline and on day 28 post\u2010application. The lip conditions, lip texture wrinkles, and hemoglobin levels were measured. Participant assessments included ratings of lip dryness, appearance, and product satisfaction.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>The herbal extracts demonstrated potential in strengthening cell adherence, providing antioxidant effect, and inducing self\u2010renewal. The Mix2 shows the most promising activity, increasing adherent protein and stemness properties, and was selected as the active ingredient for the clinical trial. In the trial, both the test and control groups experienced a significant reduction in lip roughness by day 28 compared to baseline (<jats:italic>p<\/jats:italic>\u2009&lt;\u20090.05). However, the test group exhibited a significantly greater reduction in chapped lips than the control group (<jats:italic>p<\/jats:italic>\u2009&lt;\u20090.05). No significant differences were found between the groups in terms of lip texture, wrinkle levels, or hemoglobin levels. Notably, both groups showed significant improvements in perceived lip dryness by day 28 (<jats:italic>p<\/jats:italic>\u2009&lt;\u20090.001).<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>The study findings support the therapeutic potential of the novel polyherbal lip hydrant in improving lip hydration, reducing roughness, and alleviating chapped lips (<jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" xlink:href=\"http:\/\/clinicaltrials.gov\">ClinicalTrials.gov<\/jats:ext-link> ID: NCT06475482).<\/jats:p><\/jats:sec>","DOI":"10.1111\/jocd.70041","type":"journal-article","created":{"date-parts":[[2025,3,11]],"date-time":"2025-03-11T22:50:57Z","timestamp":1741733457000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["A Randomized Double\u2010Blind Controlled Evaluation of the Therapeutic Benefits of an Herbal Lip Hydrant"],"prefix":"10.1111","volume":"24","author":[{"given":"Pimchanok","family":"Sutthiboonyapan","sequence":"first","affiliation":[{"name":"Department of Periodontology, Faculty of Dentistry Chulalongkorn University  Bangkok Thailand"},{"name":"Center of Excellence in Periodontal Disease and Dental Implant Chulalongkorn University  Bangkok Thailand"},{"name":"Center of Excellence in Genomics and Precision Dentistry Chulalongkorn University  Bangkok Thailand"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nicharat","family":"Sriratanasak","sequence":"additional","affiliation":[{"name":"Center of Excellence in Cancer Cell and Molecular Biology, Faculty of Pharmaceutical Sciences Chulalongkorn University  Bangkok Thailand"},{"name":"Department of Pharmacology and Physiology, Faculty of Pharmaceutical Sciences Chulalongkorn University  Bangkok Thailand"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bhurichaya","family":"Innets","sequence":"additional","affiliation":[{"name":"Center of Excellence in Cancer Cell and Molecular Biology, Faculty of Pharmaceutical Sciences Chulalongkorn University  Bangkok Thailand"},{"name":"Department of Pharmacology and Physiology, Faculty of Pharmaceutical Sciences Chulalongkorn University  Bangkok Thailand"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nachon","family":"Angkanaporn","sequence":"additional","affiliation":[{"name":"Faculty of Dentistry Chulalongkorn University  Bangkok Thailand"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Parinnapa","family":"Suntornchot","sequence":"additional","affiliation":[{"name":"Faculty of Dentistry Chulalongkorn University  Bangkok Thailand"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Warunya","family":"Panyain","sequence":"additional","affiliation":[{"name":"Faculty of Dentistry Chulalongkorn University  Bangkok Thailand"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Thantrira","family":"Porntaveetus","sequence":"additional","affiliation":[{"name":"Center of Excellence in Genomics and Precision Dentistry Chulalongkorn University  Bangkok Thailand"},{"name":"Department of Physiology, Faculty of Dentistry Chulalongkorn University  Bangkok Thailand"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Paswach","family":"Wiriyakijja","sequence":"additional","affiliation":[{"name":"Center of Excellence in Genomics and Precision Dentistry Chulalongkorn University  Bangkok Thailand"},{"name":"Department of Oral Medicine, Faculty of Dentistry Chulalongkorn University  Bangkok Thailand"},{"name":"Avatar Biotechnologies for Oral Health and Healthy Longevity Research Unit Chulalongkorn University  Bangkok Thailand"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3103-3249","authenticated-orcid":false,"given":"Pithi","family":"Chanvorachote","sequence":"additional","affiliation":[{"name":"Center of Excellence in Cancer Cell and Molecular Biology, Faculty of Pharmaceutical Sciences Chulalongkorn University  Bangkok Thailand"},{"name":"Department of Pharmacology and Physiology, Faculty of Pharmaceutical Sciences Chulalongkorn University  Bangkok Thailand"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2025,2,27]]},"reference":[{"key":"e_1_2_11_2_1","volume-title":"StatPearls","author":"Piccinin M. A.","year":"2024"},{"key":"e_1_2_11_3_1","doi-asserted-by":"publisher","DOI":"10.1111\/jocd.16317"},{"key":"e_1_2_11_4_1","doi-asserted-by":"publisher","DOI":"10.1159\/000077838"},{"key":"e_1_2_11_5_1","doi-asserted-by":"publisher","DOI":"10.1111\/ics.12692"},{"issue":"7","key":"e_1_2_11_6_1","first-page":"20","article-title":"Postinflammatory Hyperpigmentation: A Review of the Epidemiology, Clinical Features, and Treatment Options in Skin of Color","volume":"3","author":"Davis E. C.","year":"2010","journal-title":"Journal of Clinical and Aesthetic Dermatology"},{"key":"e_1_2_11_7_1","doi-asserted-by":"publisher","DOI":"10.1155\/2022\/7881717"},{"key":"e_1_2_11_8_1","doi-asserted-by":"publisher","DOI":"10.1002\/ajmg.a.32602"},{"key":"e_1_2_11_9_1","doi-asserted-by":"publisher","DOI":"10.1051\/medsci\/2020223"},{"key":"e_1_2_11_10_1","first-page":"13","volume-title":"Tissue Engineering (Third Edition)","author":"Pittenger M. F.","year":"2023"},{"key":"e_1_2_11_11_1","doi-asserted-by":"publisher","DOI":"10.1093\/emboj\/cdg560"},{"key":"e_1_2_11_12_1","doi-asserted-by":"publisher","DOI":"10.3390\/molecules24112130"},{"key":"e_1_2_11_13_1","doi-asserted-by":"publisher","DOI":"10.2147\/CCID.S116995"},{"key":"e_1_2_11_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biopha.2022.113559"},{"key":"e_1_2_11_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0308\u20108146(03)00155\u20109"},{"key":"e_1_2_11_16_1","doi-asserted-by":"publisher","DOI":"10.1021\/np010370g"},{"key":"e_1_2_11_17_1","doi-asserted-by":"publisher","DOI":"10.3390\/antiox8090379"},{"key":"e_1_2_11_18_1","doi-asserted-by":"publisher","DOI":"10.1111\/srt.12436"},{"key":"e_1_2_11_19_1","doi-asserted-by":"publisher","DOI":"10.1111\/ics.12583"},{"key":"e_1_2_11_20_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.emboj.7600605"},{"key":"e_1_2_11_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tiv.2019.104602"},{"key":"e_1_2_11_22_1","doi-asserted-by":"publisher","DOI":"10.4103\/jomfp.JOMFP_22_20"},{"key":"e_1_2_11_23_1","doi-asserted-by":"publisher","DOI":"10.3390\/antiox12030727"},{"key":"e_1_2_11_24_1","doi-asserted-by":"publisher","DOI":"10.4103\/phrev.phrev_55_16"},{"key":"e_1_2_11_25_1","doi-asserted-by":"publisher","DOI":"10.1089\/jmf.2013.2936"},{"key":"e_1_2_11_26_1","doi-asserted-by":"publisher","DOI":"10.2174\/1871523015666160915154443"},{"key":"e_1_2_11_27_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.foodchem.2020.127724"},{"key":"e_1_2_11_28_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms24043755"},{"key":"e_1_2_11_29_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598\u2010020\u201066723\u20101"},{"key":"e_1_2_11_30_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms222011030"},{"key":"e_1_2_11_31_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms161025476"},{"key":"e_1_2_11_32_1","doi-asserted-by":"publisher","DOI":"10.13005\/bpj\/1942"},{"key":"e_1_2_11_33_1","first-page":"6","article-title":"Clinical Assessment of Immediate and Long\u2010Term Effects of a Two\u2010Step Topical Hyaluronic Acid Lip Treatment","volume":"16","author":"Makino E. T.","year":"2017","journal-title":"Journal of Drugs in Dermatology"},{"key":"e_1_2_11_34_1","first-page":"44","article-title":"Clinical Assessment of a Combination Lip Treatment to Restore Moisturization and Fullness","volume":"2","author":"Trookman N. S.","year":"2009","journal-title":"Journal of Clinical and Aesthetic Dermatology"},{"issue":"4","key":"e_1_2_11_35_1","first-page":"395","article-title":"Type I Pro\u2010Collagen Promoting and Anti\u2010Collagenase Activities of Phyllanthus emblica Extract in Mouse Fibroblasts","volume":"60","author":"Chanvorachote P.","year":"2009","journal-title":"Journal of Cosmetic Science"},{"key":"e_1_2_11_36_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11418\u2010014\u20100824\u20101"},{"key":"e_1_2_11_37_1","doi-asserted-by":"publisher","DOI":"10.2147\/CCID.S179430"},{"key":"e_1_2_11_38_1","doi-asserted-by":"publisher","DOI":"10.1111\/jocd.12351"},{"key":"e_1_2_11_39_1","doi-asserted-by":"publisher","DOI":"10.1111\/ics.12677"},{"key":"e_1_2_11_40_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaad.2020.04.019"},{"key":"e_1_2_11_41_1","doi-asserted-by":"publisher","DOI":"10.5826\/dpc.1103a57"},{"key":"e_1_2_11_42_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.pdpdt.2023.103874"}],"container-title":["Journal of Cosmetic Dermatology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/jocd.70041","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,4,9]],"date-time":"2025-04-09T18:57:35Z","timestamp":1744225055000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/jocd.70041"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,2,27]]},"references-count":41,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2025,3]]}},"alternative-id":["10.1111\/jocd.70041"],"URL":"https:\/\/doi.org\/10.1111\/jocd.70041","archive":["Portico"],"relation":{},"ISSN":["1473-2130","1473-2165"],"issn-type":[{"value":"1473-2130","type":"print"},{"value":"1473-2165","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,2,27]]},"assertion":[{"value":"2024-10-12","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-01-31","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-02-27","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e70041"}}