{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,21]],"date-time":"2026-07-21T23:18:11Z","timestamp":1784675891181,"version":"3.55.0"},"reference-count":37,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2025,3,27]],"date-time":"2025-03-27T00:00:00Z","timestamp":1743033600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J of Cosmetic Dermatology"],"published-print":{"date-parts":[[2025,4]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Acoustic Wave Therapy (AWT) is a particular shock wave treatment that combines focused and radial shock waves, resulting in particular efficiency in the body's superficial layers. Its application before fat grafting has the potential to enhance it, favoring a better result of the graft.<\/jats:p><\/jats:sec><jats:sec><jats:title>Aims<\/jats:title><jats:p>The aim of this study was to analyze the effects of AWT on fat tissue.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Histological analysis of fat harvested from the abdomen of 40 subjects was evaluated. Twenty subjects had been pre\u2010treated with AWT, while 20 had not. Extraction and characterization of adipose\u2010derived stem cells (ADSCs) were performed for all. The multilineage differentiation capacity of extracted ADSCs and exosome extracellular vesicles' production from the fat samples were analyzed in both groups of specimens.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>All 40 specimens presented both preservation of the structure of the tissue and of the cells, particularly of adipocytes. The cell growth resulted higher for pre\u2010treated samples. All ADSCs from all the samples were able to differentiate along adipogenic, chondrogenic, and osteogenic lineages. In particular, for pre\u2010treated samples, in the adipogenic lineage, a more advanced maturation phase was observed, and in the chondrogenic lineage, a chondroid matrix was evident around chondrocyte aggregates. All the samples reacted with exosome extracellular vesicles' production to stress.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>The results highlighted that AWT aligns with the in\u2010force minimal manipulation regulations, as the tissue and cells' structure and functionality were preserved. The types of differentiation observed permitted us to speculate about possible new applications in aesthetic and regenerative medicine.<\/jats:p><\/jats:sec>","DOI":"10.1111\/jocd.70142","type":"journal-article","created":{"date-parts":[[2025,3,29]],"date-time":"2025-03-29T23:14:48Z","timestamp":1743290088000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["The Impact of Acoustic Wave Therapy on Viability and Differentiation Capacity of Human Adipose Stem Cells"],"prefix":"10.1111","volume":"24","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-6707-3306","authenticated-orcid":false,"given":"Sheila","family":"Veronese","sequence":"first","affiliation":[{"name":"Department of Neuroscience, Biomedicine, and Movement, Section of Anatomy and Histology University of Verona  Verona Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4890-129X","authenticated-orcid":false,"given":"Riccardo","family":"Ossanna","sequence":"additional","affiliation":[{"name":"Department of Neuroscience, Biomedicine, and Movement, Section of Anatomy and Histology University of Verona  Verona Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sara Ghazanfar","family":"Tehrani","sequence":"additional","affiliation":[{"name":"Department of Neuroscience, Biomedicine, and Movement, Section of Anatomy and Histology University of Verona  Verona Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Paolo","family":"Bernardi","sequence":"additional","affiliation":[{"name":"Department of Neuroscience, Biomedicine, and Movement, Section of Anatomy and Histology University of Verona  Verona Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sima","family":"Khabouri","sequence":"additional","affiliation":[{"name":"De Clinic  Milan Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Velia","family":"Cannone","sequence":"additional","affiliation":[{"name":"Department of Neuroscience, Biomedicine, and Movement, Section of Anatomy and Histology University of Verona  Verona Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maria Maddalena","family":"Nicoletti","sequence":"additional","affiliation":[{"name":"Department of Precision Medicine University of Campania \u201cLuigi Vanvitelli\u201d  Naples Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mario","family":"Goisis","sequence":"additional","affiliation":[{"name":"De Clinic  Milan Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andrea","family":"Sbarbati","sequence":"additional","affiliation":[{"name":"Department of 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