{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,21]],"date-time":"2025-11-21T17:25:01Z","timestamp":1763745901073,"version":"3.45.0"},"reference-count":20,"publisher":"Wiley","issue":"11","license":[{"start":{"date-parts":[[2025,11,18]],"date-time":"2025-11-18T00:00:00Z","timestamp":1763424000000},"content-version":"vor","delay-in-days":17,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J of Cosmetic Dermatology"],"published-print":{"date-parts":[[2025,11]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>New botulinum toxin type A (BoNT\u2010A) formulations eliminated cold\u2010chain storage before reconstitution. Nonetheless, concerns remain about BoNT\u2010A properties when stored at room temperature after reconstitution.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Aim<\/jats:title>\n                    <jats:p>This study aimed to evaluate the sterility and effectiveness of BoNT\u2010A storage at room temperature in glabellar wrinkles.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>BoNT\u2010A (Allergan Botox, Brazil) was reconstituted and maintained in a stove at 37\u00b0C for 60\u2009days and then tested for sterility according to the Pharmacopeia guidelines. Then, sixty patients aged 25\u201360\u2009years were divided into two groups: Group 1 (G1) received BoNT\u2010A immediately after reconstitution, and Group 2 (G2) received BoNT\u2010A after 7\u2009days at room temperature. Participants were assessed for wrinkle severity using the Merz Scale, satisfaction with treatment using the Face\u2010Q scale, and muscle activity by electromyography. Data were collected before injections and after 15, 30, 60, and 90\u2009days.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      Absence of turbidity evidenced no microbial growth in all periods analyzed. Both groups showed significant reductions in glabellar contraction (\n                      <jats:italic>p<\/jats:italic>\n                      \u2009&lt;\u20090.001), wrinkle severity (\n                      <jats:italic>p<\/jats:italic>\n                      \u2009&lt;\u20090.05), and improved satisfaction. G1 presented lower wrinkle severity, while G2 reported higher satisfaction (\n                      <jats:italic>p<\/jats:italic>\n                      \u2009&lt;\u20090.05).\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>Room temperature and longer storage periods may not affect OnabotulinumtoxinA sterility and clinical results.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/jocd.70554","type":"journal-article","created":{"date-parts":[[2025,11,18]],"date-time":"2025-11-18T19:20:06Z","timestamp":1763493606000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Is Cold Storage Still a Must? Evaluating Room\u2010Temperature\n                    <scp>OnabotulinumtoxinA<\/scp>\n                    for Glabellar Lines Treatment"],"prefix":"10.1111","volume":"24","author":[{"given":"Ruth Yimna Del Socorro Blanco","family":"Rodriguez","sequence":"first","affiliation":[{"name":"Ing\u00e1 University Center \u2010 UNING\u00c1  Maring\u00e1 Paran\u00e1 Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0921-342X","authenticated-orcid":false,"given":"Giancarlo","family":"De La Torre Canales","sequence":"additional","affiliation":[{"name":"Ing\u00e1 University Center \u2010 UNING\u00c1  Maring\u00e1 Paran\u00e1 Brazil"},{"name":"Egas Moniz Center for Interdisciplinary Research (CiiEM) Egas Moniz School of Health and Science  Almada Portugal"},{"name":"Division of Oral Rehabilitation, Department of Dental Medicine Karolinska Institutet  Stockholm Sweden"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"C\u00e9sar Nunes","family":"Giracca","sequence":"additional","affiliation":[{"name":"Federal University of Santa Catarina  Florian\u00f3polis Santa Catarina Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tha\u00eds Caroline Schwartz Saldanha","family":"Vaz","sequence":"additional","affiliation":[{"name":"Aesthetic Research Laboratory, Postgraduate Program in Pharmaceutical Sciences, Department of Pharmacy Federal University of Paran\u00e1  Curitiba Paran\u00e1 Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gislaine","family":"Paulovski","sequence":"additional","affiliation":[{"name":"Ing\u00e1 University Center \u2010 UNING\u00c1  Maring\u00e1 Paran\u00e1 Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lucas Alexandre de Sousa","family":"Mendes","sequence":"additional","affiliation":[{"name":"Ing\u00e1 University Center \u2010 UNING\u00c1  Maring\u00e1 Paran\u00e1 Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0591-9270","authenticated-orcid":false,"given":"Jairo Matozinho","family":"Cordeiro","sequence":"additional","affiliation":[{"name":"University Center of Associated Teaching Faculties  S\u00e3o Jo\u00e3o da Boa Vista S\u00e3o Paulo Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"F\u00e1bio Seigi","family":"Murakami","sequence":"additional","affiliation":[{"name":"Aesthetic Research Laboratory, Postgraduate Program in Pharmaceutical Sciences, Department of Pharmacy Federal University of Paran\u00e1  Curitiba Paran\u00e1 Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9961-121X","authenticated-orcid":false,"given":"Mariana Barbosa","family":"C\u00e2mara\u2010Souza","sequence":"additional","affiliation":[{"name":"Ing\u00e1 University Center \u2010 UNING\u00c1  Maring\u00e1 Paran\u00e1 Brazil"},{"name":"Araras Dental School Herm\u00ednio Ometto Foundation  Araras S\u00e3o Paulo Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2025,11,18]]},"reference":[{"key":"e_1_2_11_2_1","doi-asserted-by":"publisher","DOI":"10.1097\/IOP.0000000000002169"},{"key":"e_1_2_11_3_1","unstructured":"International Society of Aesthetic Plastic Surgery \u201cISAPS Internatiional Survey on Aesthetic\/Cosmetic Procedures Performed in 2024 [Internet] \u201d[citado 10 de setembro de 2025] Dispon\u00edvel em:https:\/\/www.isaps.org\/media\/oogpzodr\/isaps\u2010global\u2010survey_2024.pdf."},{"key":"e_1_2_11_4_1","doi-asserted-by":"publisher","DOI":"10.3390\/toxins10120535"},{"issue":"2","key":"e_1_2_11_5_1","first-page":"272","article-title":"BOTOX","volume":"55","author":"Allergan","year":"2006","journal-title":"Journal of the American Academy of Dermatology"},{"key":"e_1_2_11_6_1","unstructured":"Ipsen Biopharm Ltd. \u201cDYSPORT [Internet] \u201dAsh Road North Reino Unido; [citado 10 de setembro de 2025] Dispon\u00edvel em:https:\/\/www.ipsen.com\/websites\/ipsen_com_v2\/wp\u2010content\/uploads\/sites\/22\/2025\/06\/04112551\/Dysport_Bula\u2010Profissional\u2010de\u2010Saude\u2010HCP.pdf."},{"key":"e_1_2_11_7_1","doi-asserted-by":"publisher","DOI":"10.1097\/DSS.0000000000000303"},{"key":"e_1_2_11_8_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00266-006-0161-1"},{"key":"e_1_2_11_9_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1524-4725.2009.01158.x"},{"key":"e_1_2_11_10_1","doi-asserted-by":"publisher","DOI":"10.1093\/asj\/sju072"},{"key":"e_1_2_11_11_1","unstructured":"Merz Pharmaceuticals GmbH \u201cXEOMIN [Internet] \u201dFrankfurt\u2010Alemanha [citado 10 de setembro de 2025] Dispon\u00edvel em:https:\/\/www.saudedireta.com.br\/catinc\/drugs\/bulas\/xeomin.pdf."},{"key":"e_1_2_11_12_1","unstructured":"Revance Therapeutics Inc. \u201cDAXXIFY [Internet] \u201dNashville EUA. 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