{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,7]],"date-time":"2026-08-07T15:37:48Z","timestamp":1786117068451,"version":"build-2736575974"},"reference-count":12,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2026,2,13]],"date-time":"2026-02-13T00:00:00Z","timestamp":1770940800000},"content-version":"vor","delay-in-days":12,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,2,1]],"date-time":"2026-02-01T00:00:00Z","timestamp":1769904000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J of Cosmetic Dermatology"],"published-print":{"date-parts":[[2026,2]]},"abstract":"ABSTRACT<\/jats:title>\n \n Introduction<\/jats:title>\n Polynucleotides (PN), derived from salmon sperm DNA, are highly purified DNA fragments with reported tissue\u2010repair and anti\u2010inflammatory properties mediated through adenosine A2A receptor activation. These agents have been increasingly used in aesthetic dermatology for skin rejuvenation and scar treatment. This study aimed to evaluate longitudinal changes in periorbital appearance and patient\u2010reported outcomes following intradermal polynucleotide injections.<\/jats:p>\n <\/jats:sec>\n \n Methods<\/jats:title>\n \n This prospective observational case series included patients presenting for undereye rejuvenation who received intradermal injections of absorbable polynucleotides (7.5\u2009mg\/mL; Pluryal Silk, MD Skin Solutions, Luxembourg), with a total volume of 2\u2009mL per session. Outcomes were assessed using validated FACE\u2010Q patient\u2010reported questionnaires at baseline and at 1, 3, and 6\u2009months following treatment. Adverse events were assessed 2\u2009days after each treatment session. Statistical analysis was performed using paired\n t<\/jats:italic>\n \u2010tests.\n <\/jats:p>\n <\/jats:sec>\n \n Results<\/jats:title>\n \n Forty\u2010two patients were included. Significant improvements in lower eyelid and crow's feet appraisal scores were observed at all follow\u2010up time points compared with baseline (\n p<\/jats:italic>\n \u2009<\u20090.001). The greatest improvements and highest patient satisfaction were observed at 3\u2009months following two treatment sessions. Reported adverse events were minimal.\n <\/jats:p>\n <\/jats:sec>\n \n Conclusion<\/jats:title>\n In this prospective observational cohort, intradermal polynucleotide injections were associated with improvements in periorbital appearance and patient\u2010reported satisfaction over baseline, with minimal adverse events. These findings support further controlled studies to confirm efficacy and refine treatment protocols.<\/jats:p>\n <\/jats:sec>","DOI":"10.1111\/jocd.70736","type":"journal-article","created":{"date-parts":[[2026,2,14]],"date-time":"2026-02-14T04:56:03Z","timestamp":1771044963000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Prospective Observational Study of Polynucleotide Injections for Periorbital Rhytides"],"prefix":"10.1111","volume":"25","author":[{"given":"Georges","family":"Ziade","sequence":"first","affiliation":[{"name":"Department of Otolaryngology\u2010Head and Neck Surgery Lebanese American University School of Medicine Beirut Lebanon"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elias","family":"Keyrouz","sequence":"additional","affiliation":[{"name":"Department of Otolaryngology\u2010Head and Neck Surgery Lebanese American University School of Medicine Beirut Lebanon"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Joy","family":"El Maalouf","sequence":"additional","affiliation":[{"name":"Department of Otolaryngology\u2010Head and Neck Surgery Lebanese American University School of Medicine Beirut Lebanon"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dana","family":"Swaidan","sequence":"additional","affiliation":[{"name":"Department of Otolaryngology\u2010Head and Neck Surgery Lebanese American University School of Medicine Beirut Lebanon"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Desiree","family":"Karam","sequence":"additional","affiliation":[{"name":"Faculty of Medicine Holy Spirit University of Kaslik Kaslik Lebanon"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dayane","family":"Daou","sequence":"additional","affiliation":[{"name":"Department of Anesthesia American University of Beirut Medical Center Beirut Lebanon"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Georges","family":"Fidawi","sequence":"additional","affiliation":[{"name":"Department of Dermatology American University of Beirut Medical Center Beirut Lebanon"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,2,13]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00403-024-03088-9"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.3390\/polym17050638"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.1111\/jocd.15064"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.1093\/asj\/sjab125"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.1111\/jocd.13679"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms25158224"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.1111\/dth.12299"},{"key":"e_1_2_10_9_1","doi-asserted-by":"publisher","DOI":"10.1080\/09546634.2020.1748857"},{"key":"e_1_2_10_10_1","doi-asserted-by":"publisher","DOI":"10.1111\/jocd.16125"},{"key":"e_1_2_10_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cjprs.2022.09.015"},{"key":"e_1_2_10_12_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms17091448"},{"key":"e_1_2_10_13_1","doi-asserted-by":"publisher","DOI":"10.1111\/jocd.14578"}],"container-title":["Journal of Cosmetic Dermatology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/jocd.70736","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/jocd.70736","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/jocd.70736","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,8,6]],"date-time":"2026-08-06T13:08:59Z","timestamp":1786021739000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/jocd.70736"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,2]]},"references-count":12,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2026,2]]}},"alternative-id":["10.1111\/jocd.70736"],"URL":"https:\/\/doi.org\/10.1111\/jocd.70736","archive":["Portico"],"relation":{},"ISSN":["1473-2130","1473-2165"],"issn-type":[{"value":"1473-2130","type":"print"},{"value":"1473-2165","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,2]]},"assertion":[{"value":"2025-10-11","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-02-03","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-02-13","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e70736"}}