{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,22]],"date-time":"2026-01-22T22:20:06Z","timestamp":1769120406261,"version":"3.49.0"},"reference-count":27,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2013,5,30]],"date-time":"2013-05-30T00:00:00Z","timestamp":1369872000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Funds of China","doi-asserted-by":"crossref","award":["30872220"],"award-info":[{"award-number":["30872220"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J of Obstet and Gynaecol"],"published-print":{"date-parts":[[2013,6]]},"abstract":"Abstract<\/jats:title>Aim<\/jats:title>To investigate the effect and mechanism of hepatitis B<\/jats:styled-content> virus (HBV<\/jats:styled-content>) infection on the human choriocarcinoma cell line, JEG<\/jats:styled-content>\u20103, in relation to apoptosis and intrauterine infection.<\/jats:p><\/jats:sec>Material and Methods<\/jats:title>HBV\u2010DNA<\/jats:styled-content> serum was used to infect the choriocarcinoma cell line, JEG<\/jats:styled-content>\u20103, in vitro<\/jats:italic>. Real\u2010time fluorescence quantitative PCR<\/jats:styled-content> (RT\u2010PCR<\/jats:styled-content>) was then employed to detect intracellular replication of HBV DNA<\/jats:styled-content>. Cells were also stained with A<\/jats:styled-content>nnexin\u2010V<\/jats:styled-content> and propidium iodide (PI<\/jats:styled-content>) to identify the stages of apoptosis following infection. In addition, reverse transcription PCR<\/jats:styled-content> was used to detect intracellular HBx<\/jats:styled-content><\/jats:italic>\u2005mRNA<\/jats:styled-content> levels, and W<\/jats:styled-content>estern blotting and immunohistochemistry were used to detect changes in the intracellular expression of HBxAg<\/jats:styled-content> and phosphatidylinositol kinase 3 (PI3K<\/jats:styled-content>). Flow cytometry was also used to detect the intracellular levels of phosphorylated AKT<\/jats:styled-content> (pAKT<\/jats:styled-content>).<\/jats:p><\/jats:sec>Results<\/jats:title>After JEG<\/jats:styled-content>\u20103 cells were infected with HBV<\/jats:styled-content>\u2005in vitro<\/jats:italic>, HBV DNA<\/jats:styled-content> was detected. The percentage of cells in early and late stage apoptosis also decreased significantly. Expression of HBx<\/jats:styled-content><\/jats:italic>\u2005mRNA<\/jats:styled-content> and HBxAg<\/jats:styled-content> were detected, and intracellular levels of PI3K<\/jats:styled-content> and pAKT<\/jats:styled-content> were observed to significantly increase.<\/jats:p><\/jats:sec>Conclusion<\/jats:title>HBV<\/jats:styled-content> infected JEG<\/jats:styled-content>\u20103 cells in vitro<\/jats:italic>, resulting in an inhibition of early and late stage apoptosis. In addition, the HBxAg<\/jats:styled-content>\/PI3K<\/jats:styled-content>\/pAKT<\/jats:styled-content> pathway is a possible mechanism mediating this inhibition of apoptosis, and the infection of the placenta by HBV<\/jats:styled-content>.<\/jats:p><\/jats:sec>","DOI":"10.1111\/jog.12046","type":"journal-article","created":{"date-parts":[[2013,5,30]],"date-time":"2013-05-30T01:33:38Z","timestamp":1369877618000},"page":"1200-1211","source":"Crossref","is-referenced-by-count":7,"title":["Effect of hepatitis B virus infection on apoptosis of a human choriocarcinoma cell line in vitro<\/i>"],"prefix":"10.1111","volume":"39","author":[{"given":"Guiqin","family":"Bai","sequence":"first","affiliation":[{"name":"Department of Gynecology and Obstetrics The First Affiliated Hospital of Medical College of Xi'an Jiaotong University Xi'an China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fengping","family":"Fu","sequence":"additional","affiliation":[{"name":"Department of 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