{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,25]],"date-time":"2026-02-25T01:14:45Z","timestamp":1771982085582,"version":"3.50.1"},"reference-count":18,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2013,7,4]],"date-time":"2013-07-04T00:00:00Z","timestamp":1372896000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"Funda\u00e7\u00e3o de Amparo \u00e0 Pesquisa do Estado de S\u00e3o Paulo, FAPESP","award":["2009\/54508-0"],"award-info":[{"award-number":["2009\/54508-0"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J of Obstet and Gynaecol"],"published-print":{"date-parts":[[2013,9]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Aim\u2002<\/jats:title><jats:p>The aim of this study was to evaluate the passage of fetal erythrocytes into the maternal circulation after invasive obstetric procedures, using the <jats:styled-content style=\"fixed-case\">K<\/jats:styled-content>leihauer\u2013<jats:styled-content style=\"fixed-case\">B<\/jats:styled-content>etke test, flow cytometry and \u03b1\u2010fetoprotein concentration in maternal blood.<\/jats:p><\/jats:sec><jats:sec><jats:title>Material and Methods<\/jats:title><jats:p>This was a prospective descriptive study on patients who underwent: amniocentesis, cordocentesis, chorionic villus sample, amniotic infusion, bladder drainage and ventricular\u2013amniotic shunt to investigate the karyotype; treatment for hydrocephalus, oligohydramnios, obstructive uropathy and polyhydramnios; and investigation of lung maturity. Maternal blood samples were collected before and 60\u2009min after the invasive obstetric procedure in order to evaluate the passage of fetal erythrocytes using the <jats:styled-content style=\"fixed-case\">K<\/jats:styled-content>leihauer\u2013<jats:styled-content style=\"fixed-case\">B<\/jats:styled-content>etke test, flow cytometry and \u03b1\u2010fetoprotein concentration.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>In total, 43 invasive obstetric procedures were performed. The procedures performed were: 27 cases of amniocentesis (62.7%), seven cases of cordocentesis (16.2%), four chorionic villus samples (9.4%), two amniotic infusions (4.7%), two ventricular\u2013amniotic shunts and one bladder drainage (2.3%). After one case of cordocentesis with two puncture attempts via the placenta, a significant increase in fetal erythrocytes was detected using the three methods. After another cordocentesis with one puncture via the placenta, a significant increase in fetal erythrocytes was detected using flow cytometry and \u03b1\u2010fetoprotein concentration, but not through the <jats:styled-content style=\"fixed-case\">K<\/jats:styled-content>leihauer\u2013<jats:styled-content style=\"fixed-case\">B<\/jats:styled-content>etke test. The other 41 samples did not show any significant increase in fetal erythrocytes in the maternal blood.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Invasive obstetric procedures performed during prenatal care are safe when performed by experienced professionals with the proper technique, with minimal chance of passage of fetal erythrocytes into the maternal compartment.<\/jats:p><\/jats:sec>","DOI":"10.1111\/jog.12073","type":"journal-article","created":{"date-parts":[[2013,7,4]],"date-time":"2013-07-04T10:40:58Z","timestamp":1372934458000},"page":"1374-1382","source":"Crossref","is-referenced-by-count":18,"title":["Evaluation of passage of fetal erythrocytes into maternal circulation after invasive obstetric procedures"],"prefix":"10.1111","volume":"39","author":[{"given":"Daniela","family":"Meleti","sequence":"first","affiliation":[{"name":"Department of Obstetrics Federal University of S\u00e3o Paulo (UNIFESP)  S\u00e3o Paulo SP 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