{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,1]],"date-time":"2026-08-01T11:01:11Z","timestamp":1785582071111,"version":"3.56.0"},"reference-count":43,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2013,10,7]],"date-time":"2013-10-07T00:00:00Z","timestamp":1381104000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"LOCUS MEDICUS S.A."}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J of Obstet and Gynaecol"],"published-print":{"date-parts":[[2014,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Aim<\/jats:title><jats:p>At present, routine laboratory investigation of the infectious agents implicated in female genital infections is mainly based on culture\/direct fluorescence antibody (<jats:styled-content style=\"fixed-case\">DFA<\/jats:styled-content>) (immunofluorescence antibody test) results of cervicovaginal secretions. In this study the use of the menstrual tissue is introduced for the molecular detection of pathogens which are implicated in female infertility.<\/jats:p><\/jats:sec><jats:sec><jats:title>Material and Methods<\/jats:title><jats:p>Cervicovaginal secretions and menstrual tissue samples of 87 women (mean age 34.07\u2009\u00b1\u20095.17) experiencing infertility problems were screened for <jats:italic><jats:styled-content style=\"fixed-case\">C<\/jats:styled-content>hlamydia trachomatis, <jats:styled-content style=\"fixed-case\">U<\/jats:styled-content>reaplasma urealyticum<\/jats:italic> and <jats:italic><jats:styled-content style=\"fixed-case\">M<\/jats:styled-content>ycoplasma hominis<\/jats:italic> presence using polymerase chain reaction (<jats:styled-content style=\"fixed-case\">PCR<\/jats:styled-content>, light cycler<jats:styled-content style=\"fixed-case\">\u2010PCR<\/jats:styled-content>). Cervicovaginal secretions were also tested by the culture\/<jats:styled-content style=\"fixed-case\">DFA<\/jats:styled-content> technique. The results were compared using the binomial test.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>In the overall study group, the prevalence of <jats:italic><jats:styled-content style=\"fixed-case\">C<\/jats:styled-content>. trachomatis<\/jats:italic> was 25.3%, 18.3%, and 13.8%, the prevalence of <jats:italic><jats:styled-content style=\"fixed-case\">U<\/jats:styled-content>. urealyticum<\/jats:italic> was 18.3%, 16.09% and 12.6% and the prevalence of <jats:italic><jats:styled-content style=\"fixed-case\">M<\/jats:styled-content>. hominis<\/jats:italic> was 13.7%, 19.5% and 8.0% in the menstrual tissue, cervicovaginal secretions using <jats:styled-content style=\"fixed-case\">PCR<\/jats:styled-content> and cervicovaginal secretions culture\/<jats:styled-content style=\"fixed-case\">DFA<\/jats:styled-content>, respectively. A statistically significant difference was revealed between the two methods for all three microbes and between menstrual tissue and cervicovaginal secretions <jats:styled-content style=\"fixed-case\">PCR<\/jats:styled-content> for chlamydia.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>The use of menstrual tissue along with the <jats:styled-content style=\"fixed-case\">PCR<\/jats:styled-content> method seems to be an effective and thus novel alternative for the investigation of the infectious agents lying in the genital tract. One of the main advantages of this technique compared to cervicovaginal secretions is that it is non\u2010invasive and the sample can be collected at home, thus allowing the early detection and treatment of a condition that can otherwise lead to serious consequences, such as tubal obstruction, pelvic inflammatory disease, ectopic pregnancy, spontaneous abortions and unexplained infertility.<\/jats:p><\/jats:sec>","DOI":"10.1111\/jog.12165","type":"journal-article","created":{"date-parts":[[2013,10,12]],"date-time":"2013-10-12T02:05:06Z","timestamp":1381543506000},"page":"237-242","source":"Crossref","is-referenced-by-count":22,"title":["Molecular investigation of menstrual tissue for the presence of <i><scp>C<\/scp>hlamydia trachomatis, <scp>U<\/scp>reaplasma urealyticum<\/i> and <i><scp>M<\/scp>ycoplasma hominis<\/i> collected by women with a history of infertility"],"prefix":"10.1111","volume":"40","author":[{"given":"I. 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