{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,24]],"date-time":"2026-02-24T16:51:28Z","timestamp":1771951888669,"version":"3.50.1"},"reference-count":32,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2013,11,18]],"date-time":"2013-11-18T00:00:00Z","timestamp":1384732800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J of Obstet and Gynaecol"],"published-print":{"date-parts":[[2014,3]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Aim<\/jats:title><jats:p>Hypertensive disorder complicating pregnancy (<jats:styled-content style=\"fixed-case\">HDCP<\/jats:styled-content>) is one of the most frequent and serious pregnancy\u2010related diseases, which is closely related to disorders of the maternal immune system, especially the local immune microenvironment of the maternal\u2013fetal interface. Uterine decidual natural killer (<jats:styled-content style=\"fixed-case\">dNK<\/jats:styled-content>) cells are the major immune cells in the maternal\u2013fetal interface and they play an important role in establishing and maintaining a normal pregnancy. The aim of this study was to investigate the phenotype and function of <jats:styled-content style=\"fixed-case\">dNK<\/jats:styled-content> cells from women with <jats:styled-content style=\"fixed-case\">HDCP<\/jats:styled-content>.<\/jats:p><\/jats:sec><jats:sec><jats:title>Material and Methods<\/jats:title><jats:p>Decidual tissues were collected from women with normal pregnancy (normal control group, <jats:italic>n<\/jats:italic>\u2009=\u200915 cases) and <jats:styled-content style=\"fixed-case\">HDCP<\/jats:styled-content> (<jats:styled-content style=\"fixed-case\">HDCP<\/jats:styled-content> group, <jats:italic>n<\/jats:italic>\u2009=\u200920 cases), respectively. The mononuclear cells were extracted from tissues and flow cytometry (<jats:styled-content style=\"fixed-case\">FCM<\/jats:styled-content>) was utilized to sort out dNK cells. The phenotypes of <jats:styled-content style=\"fixed-case\">dNK<\/jats:styled-content> cells (<jats:styled-content style=\"fixed-case\">CD56<jats:sup>bright<\/jats:sup>CD16<\/jats:styled-content><jats:sup>\u2212<\/jats:sup><jats:styled-content style=\"fixed-case\">CD3<\/jats:styled-content><jats:sup>\u2212<\/jats:sup> vs <jats:styled-content style=\"fixed-case\">CD56<jats:sup>dim<\/jats:sup>CD16<\/jats:styled-content><jats:sup>+<\/jats:sup><jats:styled-content style=\"fixed-case\">CD3<\/jats:styled-content><jats:sup>\u2212<\/jats:sup>) were detected by <jats:styled-content style=\"fixed-case\">FCM<\/jats:styled-content>. After being co\u2010cultured with Phorbol 12\u2010myristate 13\u2010acetate, ionomycin and monensin, the expression level of interferon (<jats:styled-content style=\"fixed-case\">IFN<\/jats:styled-content>)\u2010\u03b3 in the <jats:styled-content style=\"fixed-case\">dNK<\/jats:styled-content> cells was detected by <jats:styled-content style=\"fixed-case\">FCM<\/jats:styled-content>.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>The phenotypes of <jats:styled-content style=\"fixed-case\">dNK<\/jats:styled-content> cells from the two groups were dominated by the <jats:styled-content style=\"fixed-case\">CD56<jats:sup>bright<\/jats:sup>CD16<\/jats:styled-content><jats:sup>\u2212<\/jats:sup><jats:styled-content style=\"fixed-case\">CD3<\/jats:styled-content><jats:sup>\u2212<\/jats:sup> subset, with no significant statistical difference (<jats:italic><jats:styled-content style=\"fixed-case\">P<\/jats:styled-content><\/jats:italic>\u2009&lt;\u20090.05). The expression level of <jats:styled-content style=\"fixed-case\">IFN<\/jats:styled-content>\u2010\u03b3 in the <jats:styled-content style=\"fixed-case\">dNK<\/jats:styled-content> cells from women with <jats:styled-content style=\"fixed-case\">HDCP<\/jats:styled-content> was on a lower trend than those from women with normal pregnancy, having significant statistical difference (<jats:italic><jats:styled-content style=\"fixed-case\">P<\/jats:styled-content><\/jats:italic>\u2009=\u20090.000\u2009&lt;\u20090.05).<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>Our results indicated that although the phenotype of <jats:styled-content style=\"fixed-case\">dNK<\/jats:styled-content> cells from women with <jats:styled-content style=\"fixed-case\">HDCP<\/jats:styled-content> is of no difference, their functions are abnormal. Impaired cell function leads to a lower expression level of <jats:styled-content style=\"fixed-case\">IFN<\/jats:styled-content>\u2010\u03b3 and this may account for one of the pathogeneses of <jats:styled-content style=\"fixed-case\">HDCP<\/jats:styled-content>.<\/jats:p><\/jats:sec>","DOI":"10.1111\/jog.12216","type":"journal-article","created":{"date-parts":[[2013,11,19]],"date-time":"2013-11-19T04:56:30Z","timestamp":1384836990000},"page":"670-676","source":"Crossref","is-referenced-by-count":11,"title":["Expression of interferon\u2010\u03b3 in decidual natural killer cells from women with hypertensive disorder complicating pregnancy"],"prefix":"10.1111","volume":"40","author":[{"given":"Juan","family":"Zhou","sequence":"first","affiliation":[{"name":"Department of Obstetrics and Gynecology The First Affiliated Hospital of JINAN University  Guangzhou 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