{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,1]],"date-time":"2025-11-01T17:21:56Z","timestamp":1762017716158,"version":"3.37.3"},"reference-count":32,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2013,12,10]],"date-time":"2013-12-10T00:00:00Z","timestamp":1386633600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100000156","name":"FRSQ","doi-asserted-by":"crossref","id":[{"id":"10.13039\/501100000156","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J of Obstet and Gynaecol"],"published-print":{"date-parts":[[2014,3]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Aim<\/jats:title><jats:p>The aim of the present experimental study was to assess the tocolytic effect of eicosanoids on myometrium from non\u2010pregnant and pregnant rats with or without an induced inflammatory condition.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Three hundred myometrial rings were obtained by median laparotomy from 50 <jats:styled-content style=\"fixed-case\">S<\/jats:styled-content>prague\u2013<jats:styled-content style=\"fixed-case\">D<\/jats:styled-content>awley rats divided into three groups: (i) non\u2010pregnant (<jats:italic>n<\/jats:italic>\u2009=\u200915); (ii) pregnant in absence (<jats:italic>n<\/jats:italic>\u2009=\u200920); or (iii) pregnant in presence (<jats:italic>n<\/jats:italic>\u2009=\u200915) of lipopolysaccharide treatment, timed at 22\u2009days of pregnancy. Spontaneous contractile activities were compared by isometric tension measurements. The effects of epoxy\u2010 and hydroxyeicosanoids derived from arachidonic acid as well as specific enzyme inhibitors were assessed. Changes were expressed as percentage of basal activity by calculating the area under the curve as a function of drug concentration and compared to the effect of the vehicle.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>A decrease in contractile activity ranging 10\u201325% was observed upon addition of epoxy\u2010 and hydroxyeicosanoids. Increasing epoxyeicosanoid bioavailability by inhibiting their degradation induced a tocolytic effect in the non\u2010pregnant group (20%) and in inflammation\u2010induced condition (40%). There was a significant difference in reactivity between groups and pregnancy condition. Semiquantification of metabolic enzymes that produce (cytochrome <jats:styled-content style=\"fixed-case\">P<\/jats:styled-content>\u2010450 epoxygenase) and degrade (soluble epoxide hydrolase) epoxyeicosanoids by western blot analysis revealed that these enzymes were mainly detected in the non\u2010pregnant group.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Eicosanoids can modify myometrial reactivity and their presence and effects are amplified in non\u2010pregnant and in inflammation\u2010induced condition. Our data suggest that in contrast to prostaglandins, epoxyeicosatrienoic acids are likely involved in the quiescence phase of parturition because they reduce the rhythmic contractile activity of uterine tissues in pregnant rats.<\/jats:p><\/jats:sec>","DOI":"10.1111\/jog.12247","type":"journal-article","created":{"date-parts":[[2013,12,10]],"date-time":"2013-12-10T08:36:42Z","timestamp":1386664602000},"page":"661-669","source":"Crossref","is-referenced-by-count":6,"title":["Effect of cytochrome <scp>P<\/scp>\u2010450 epoxygenase and hydroxylase metabolites on rat myometrium contractility in non\u2010pregnancy, late pregnancy and late pregnancy under inflammatory conditions"],"prefix":"10.1111","volume":"40","author":[{"given":"St\u00e9phanie","family":"Corriveau","sequence":"first","affiliation":[{"name":"Obstetrics and Gynecology CRC E\u2010Lebel \u2013 CHUS  Sherbrooke Quebec Canada"},{"name":"Physiology and Biophysics Faculty of Medicine and Health Sciences Universit\u00e9 de 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