{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T17:44:53Z","timestamp":1760204693833,"version":"3.37.3"},"reference-count":13,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2014,4,22]],"date-time":"2014-04-22T00:00:00Z","timestamp":1398124800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100008968","name":"National Research Council, Sri Lanka","doi-asserted-by":"crossref","award":["05-15"],"award-info":[{"award-number":["05-15"]}],"id":[{"id":"10.13039\/100008968","id-type":"DOI","asserted-by":"crossref"}]},{"name":"Human Genetics Unit Development Fund"},{"name":"University of Jaffna, Sri Lanka","award":["IRQUE\/JA\/CON\/026"],"award-info":[{"award-number":["IRQUE\/JA\/CON\/026"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J of Obstet and Gynaecol"],"published-print":{"date-parts":[[2014,5]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Aim<\/jats:title><jats:p>We have previously reported that polymorphism in the epidermal growth factor (<jats:italic><jats:styled-content style=\"fixed-case\">EGF<\/jats:styled-content><\/jats:italic>) gene is associated with pre\u2010eclampsia and birthweight based on case\u2013control association studies involving two single nucleotide polymorphisms (<jats:styled-content style=\"fixed-case\">SNP<\/jats:styled-content>). We extended that work to investigate other <jats:styled-content style=\"fixed-case\">SNP<\/jats:styled-content> in the <jats:italic><jats:styled-content style=\"fixed-case\">EGF<\/jats:styled-content><\/jats:italic> gene for their association with pre\u2010eclampsia and the weight of babies at birth.<\/jats:p><\/jats:sec><jats:sec><jats:title>Material and Methods<\/jats:title><jats:p>A population\u2010based <jats:styled-content style=\"fixed-case\">DNA<\/jats:styled-content> collection was genotyped to determine whether the selected <jats:styled-content style=\"fixed-case\">SNP<\/jats:styled-content> were polymorphic in the study population. In total, 175 women with pre\u2010eclampsia and 171 matched normotensive controls were genotyped for the polymorphic <jats:styled-content style=\"fixed-case\">SNP<\/jats:styled-content> using polymerase chain reaction\/restriction fragment length polymorphism and <jats:styled-content style=\"fixed-case\">MassARRAY S<\/jats:styled-content>equenom <jats:styled-content style=\"fixed-case\">iPLEX<\/jats:styled-content> methodology.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>The rs3756261<jats:styled-content style=\"fixed-case\">A<\/jats:styled-content>, rs4444903<jats:styled-content style=\"fixed-case\">G<\/jats:styled-content>, rs2237051<jats:styled-content style=\"fixed-case\">G<\/jats:styled-content> haplotype was associated with the highest increased risk of pre\u2010eclampsia (odds ratio: 3.70, 95% confidence interval: 1.38\u20139.94; <jats:italic>P<\/jats:italic>\u2009=\u20090.016). The rs3756261<jats:styled-content style=\"fixed-case\">A<\/jats:styled-content> allele was the one that contributed to this high degree of significance. The same allele was present in the haplotype rs3756261<jats:styled-content style=\"fixed-case\">A<\/jats:styled-content>, rs11568943<jats:styled-content style=\"fixed-case\">G<\/jats:styled-content>, rs2237051<jats:styled-content style=\"fixed-case\">G<\/jats:styled-content>, rs11569017<jats:styled-content style=\"fixed-case\">A<\/jats:styled-content>, rs4698803<jats:styled-content style=\"fixed-case\">T<\/jats:styled-content> (likelihood ratio statistic\u2009=\u200920.4671, d.f.\u2009=\u20093, <jats:italic>P<\/jats:italic>\u2010value\u2009=\u20090.0001), which was associated with the lower birthweight.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>In this study we found further evidence for the association of polymorphism in the <jats:italic><jats:styled-content style=\"fixed-case\">EGF<\/jats:styled-content><\/jats:italic> gene with pre\u2010eclampsia and the weight of babies at birth and identified rs3756261<jats:styled-content style=\"fixed-case\">A<\/jats:styled-content>&gt;<jats:styled-content style=\"fixed-case\">G<\/jats:styled-content> as the <jats:styled-content style=\"fixed-case\">SNP<\/jats:styled-content> that makes the most significant contribution to this association. Bioinformatic analysis showed that this effect may be mediated by caudal type homeohox\u20102, a transcriptional repressor expressed in the trophoblast, for which a binding site is created at this polymorphic site when the rs3756261<jats:styled-content style=\"fixed-case\">A<\/jats:styled-content> allele is present.<\/jats:p><\/jats:sec>","DOI":"10.1111\/jog.12362","type":"journal-article","created":{"date-parts":[[2014,4,23]],"date-time":"2014-04-23T04:12:46Z","timestamp":1398226366000},"page":"1235-1242","source":"Crossref","is-referenced-by-count":10,"title":["Polymorphism in the epidermal growth factor gene is associated with pre\u2010eclampsia and low birthweight"],"prefix":"10.1111","volume":"40","author":[{"given":"Thurayratnam","family":"Chenthuran","sequence":"first","affiliation":[{"name":"Human Genetics Unit Faculty of Medicine University of Colombo  Colombo Sri Lanka"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gayani Harendra","family":"Galhenagey","sequence":"additional","affiliation":[{"name":"Human Genetics Unit Faculty of Medicine University of Colombo  Colombo Sri Lanka"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rohan W.","family":"Jayasekara","sequence":"additional","affiliation":[{"name":"Human Genetics Unit Faculty of Medicine University of Colombo  Colombo Sri Lanka"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Vajira H. W.","family":"Dissanayake","sequence":"additional","affiliation":[{"name":"Human Genetics Unit Faculty of Medicine University of Colombo  Colombo Sri Lanka"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2014,4,22]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1042\/CS20050323"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1447-0756.2008.00926.x"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1093\/molehr\/gam011"},{"key":"e_1_2_7_5_1","first-page":"131","article-title":"Fetal epidermal growth factor susceptibility haplotype associated with fetal growth restriction","volume":"45","author":"Faraj LE","year":"2008","journal-title":"Ann Clin Biochem"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1471-0528.2004.00128.x"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1447-0756.2007.00475.x"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1471-0528.1986.tb07830.x"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1349-7006.2007.00463.x"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/14.21.8427"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1159\/000119108"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.onc.1210258"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1093\/hmg\/ddq128"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1447-0756.2011.01700.x"}],"container-title":["Journal of Obstetrics and Gynaecology Research"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fjog.12362","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fjog.12362","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/obgyn.onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/jog.12362","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,2]],"date-time":"2023-10-02T21:32:42Z","timestamp":1696282362000},"score":1,"resource":{"primary":{"URL":"https:\/\/obgyn.onlinelibrary.wiley.com\/doi\/10.1111\/jog.12362"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2014,4,22]]},"references-count":13,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2014,5]]}},"alternative-id":["10.1111\/jog.12362"],"URL":"https:\/\/doi.org\/10.1111\/jog.12362","archive":["Portico"],"relation":{},"ISSN":["1341-8076","1447-0756"],"issn-type":[{"type":"print","value":"1341-8076"},{"type":"electronic","value":"1447-0756"}],"subject":[],"published":{"date-parts":[[2014,4,22]]}}}