{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,6]],"date-time":"2026-03-06T00:09:57Z","timestamp":1772755797420,"version":"3.50.1"},"reference-count":31,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2014,6,2]],"date-time":"2014-06-02T00:00:00Z","timestamp":1401667200000},"content-version":"vor","delay-in-days":1,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J of Obstet and Gynaecol"],"published-print":{"date-parts":[[2014,6]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Aim<\/jats:title><jats:p><jats:styled-content style=\"fixed-case\">p16INK4a<\/jats:styled-content> and argentophilic nucleolus organizer region (<jats:styled-content style=\"fixed-case\">AgNOR<\/jats:styled-content>) can be used as markers for progression of cervical intraepithelial neoplasia grade 1 (<jats:styled-content style=\"fixed-case\">CIN1<\/jats:styled-content>) of the uterine cervix. Our objective was to study the predictive value of the <jats:styled-content style=\"fixed-case\">AgNOR<\/jats:styled-content> technique as a progression marker of <jats:styled-content style=\"fixed-case\">CIN1<\/jats:styled-content> and its correlation with <jats:styled-content style=\"fixed-case\">p16INK4A<\/jats:styled-content>.<\/jats:p><\/jats:sec><jats:sec><jats:title>Material and Methods<\/jats:title><jats:p>One uterine cervix biopsy from each of 75 patients with diagnosis of <jats:styled-content style=\"fixed-case\">CIN<\/jats:styled-content>1 was selected. All of these patients underwent a second biopsy, and these were also used for the study.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>The second biopsies showed: regression (20 patients), persistent <jats:styled-content style=\"fixed-case\">CIN<\/jats:styled-content>1 (38 patients), progression to <jats:styled-content style=\"fixed-case\">CIN<\/jats:styled-content>2 (10 patients) and progression to <jats:styled-content style=\"fixed-case\">CIN<\/jats:styled-content>3 (seven patients). <jats:styled-content style=\"fixed-case\">p16INK4A<\/jats:styled-content> showed reactivity in 67 of the 75 first <jats:styled-content style=\"fixed-case\">CIN1<\/jats:styled-content> biopsies: 12 of the 20 cases that cleared the lesions and the 55 cases with persistent or progressive lesions were positive for <jats:styled-content style=\"fixed-case\">p16INK4a<\/jats:styled-content> (specificity: 40%; sensitivity: 100%; positive predictive value [<jats:styled-content style=\"fixed-case\">PPV<\/jats:styled-content>]: 82%; negative predictive value [<jats:styled-content style=\"fixed-case\">NPV<\/jats:styled-content>]: 100%). Samples with <jats:styled-content style=\"fixed-case\">AgNOR<\/jats:styled-content> areas less than 3.0 \u03bc<jats:sup>2<\/jats:sup> returned in all cases, but patients whose lesions persisted or progressed to <jats:styled-content style=\"fixed-case\">CIN2<\/jats:styled-content>\/<jats:styled-content style=\"fixed-case\">CIN3<\/jats:styled-content>, showed <jats:styled-content style=\"fixed-case\">AgNOR<\/jats:styled-content> areas greater than 3.0\u2009\u03bc<jats:sup>2<\/jats:sup> in 50\/55 cases (specificity: 100%; sensitivity: 91%; <jats:styled-content style=\"fixed-case\">PPV<\/jats:styled-content>: 100%; <jats:styled-content style=\"fixed-case\">NPV<\/jats:styled-content>: 80%).<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p><jats:styled-content style=\"fixed-case\">p16INK4a<\/jats:styled-content> is expressed in a high percentage of returning lesions. <jats:styled-content style=\"fixed-case\">AgNOR<\/jats:styled-content> might be a better marker of proliferation of <jats:styled-content style=\"fixed-case\">CIN1<\/jats:styled-content> than <jats:styled-content style=\"fixed-case\">p16INK4a<\/jats:styled-content> (<jats:styled-content style=\"fixed-case\">PPV<\/jats:styled-content>\u2009=\u2009100%), which means that a value greater than 3.0\u2009\u03bc<jats:sup>2<\/jats:sup> indicates the persistence or progression of the lesion. As its <jats:styled-content style=\"fixed-case\">NPV<\/jats:styled-content> is 80%, a value of <jats:styled-content style=\"fixed-case\">AgNOR<\/jats:styled-content> area less than 3.0\u2009\u03bc<jats:sup>2<\/jats:sup> in <jats:styled-content style=\"fixed-case\">CIN1<\/jats:styled-content> leaves a margin of doubt about the future behavior of the lesion.<\/jats:p><\/jats:sec>","DOI":"10.1111\/jog.12380","type":"journal-article","created":{"date-parts":[[2014,6,3]],"date-time":"2014-06-03T03:14:07Z","timestamp":1401765247000},"page":"1717-1724","source":"Crossref","is-referenced-by-count":5,"title":["Argentophilic nucleolus organizer region as a proliferation marker in cervical intraepithelial neoplasia grade 1 of the uterine cervix"],"prefix":"10.1111","volume":"40","author":[{"given":"Fernando","family":"Guerra","sequence":"first","affiliation":[{"name":"Department of Clinical Biochemistry Leloir Institute Foundation \u2013 CONICET  Buenos Aires 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