{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,5,20]],"date-time":"2025-05-20T15:12:43Z","timestamp":1747753963885},"reference-count":27,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2014,9,2]],"date-time":"2014-09-02T00:00:00Z","timestamp":1409616000000},"content-version":"vor","delay-in-days":1,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J of Obstet and Gynaecol"],"published-print":{"date-parts":[[2014,9]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Aim<\/jats:title><jats:p>Myosin is involved in cell contraction and motility, but it is unclear whether it is involved in cell proliferation in uterine myoma. In this study therefore we aimed to explore the role of myosin in uterine myoma.<\/jats:p><\/jats:sec><jats:sec><jats:title>Material and Methods<\/jats:title><jats:p>Immunohistochemistry and real\u2010time polymerase chain reaction were used to determine the expression of myosin light chain (<jats:styled-content style=\"fixed-case\">MLC<\/jats:styled-content>), myosin heavy chain (<jats:styled-content style=\"fixed-case\">MHC<\/jats:styled-content>) and myosin light chain kinase (<jats:styled-content style=\"fixed-case\">MLCK<\/jats:styled-content>) in patient uterine myoma and adjacent smooth muscle tissue. Human uterine fibroid cells were isolated and cultured <jats:italic>in vitro<\/jats:italic>, myosin heavy chain 11 (<jats:styled-content style=\"fixed-case\">MHC<\/jats:styled-content> subtype expressed in uterine fibroid cells) was knocked down by <jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content> interference to reduce the expression of myosin, then cell proliferation was determined by the methyl thiazol tetrazolium bromide method. To explore the possible mechanism of reduced cell proliferation after myosin heavy chain 11 knockdown, the downstream proteins collagen <jats:styled-content style=\"fixed-case\">I<\/jats:styled-content>, insulin\u2010like growth factor\u20101, fibronectin and proteoglycans were analyzed.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Expression of <jats:styled-content style=\"fixed-case\">MLC<\/jats:styled-content>, <jats:styled-content style=\"fixed-case\">MHC<\/jats:styled-content>, <jats:styled-content style=\"fixed-case\">MLCK<\/jats:styled-content> and p\u2010<jats:styled-content style=\"fixed-case\">MLCK<\/jats:styled-content> in uterine myoma cells was significantly higher than in adjacent smooth muscle cells. After knockdown of <jats:styled-content style=\"fixed-case\">MHC<\/jats:styled-content>, smooth muscle cell proliferation decreased, and the production of collagen <jats:styled-content style=\"fixed-case\">I<\/jats:styled-content>, insulin\u2010like growth factor\u20101 and fibronectin was also reduced, but proteoglycans did not show any significant change.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Myosin is overexpressed in uterine myoma, and the overexpression of myosin is associated with both uterine contraction and tumor development of uterine myoma.<\/jats:p><\/jats:sec>","DOI":"10.1111\/jog.12461","type":"journal-article","created":{"date-parts":[[2014,9,2]],"date-time":"2014-09-02T14:10:06Z","timestamp":1409667006000},"page":"2051-2057","source":"Crossref","is-referenced-by-count":7,"title":["Overexpression of myosin is associated with the development of uterine myoma"],"prefix":"10.1111","volume":"40","author":[{"given":"Wenchao","family":"Zhang","sequence":"first","affiliation":[{"name":"Center for Reproductive Medicine Provincial Hospital Affiliated to Shandong University  Jinan China"},{"name":"Department of Obstetrics and Gynecology Yangpu District Central Hospital  Shanghai China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zhongping","family":"Cheng","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynecology Yangpu District Central Hospital  Shanghai China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiaoyan","family":"Qu","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynecology Yangpu District Central Hospital  Shanghai China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hong","family":"Dai","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynecology Yangpu District Central Hospital  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