{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,15]],"date-time":"2026-07-15T13:52:59Z","timestamp":1784123579881,"version":"3.55.0"},"reference-count":22,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2014,11,25]],"date-time":"2014-11-25T00:00:00Z","timestamp":1416873600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100004330","name":"Japan Smoking Research Foundation","doi-asserted-by":"crossref","id":[{"id":"10.13039\/501100004330","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J of Obstet and Gynaecol"],"published-print":{"date-parts":[[2015,5]]},"abstract":"Abstract<\/jats:title>Aim<\/jats:title>S<\/jats:styled-content>hakuyaku\u2010kanzo\u2010to<\/jats:italic>, a K<\/jats:styled-content>ampo<\/jats:italic> medicine composed equally of shakuyaku<\/jats:italic> and kanzo<\/jats:italic>, is an antispasmodic drug that can inhibit contraction of uterine smooth muscles in pregnant women and rats. We aimed to test the inhibitory effects of water\u2010 and lipid\u2010soluble extracts of shakuyaku\u2010kanzo\u2010to<\/jats:italic>, shakuyaku<\/jats:italic>, and kanzo<\/jats:italic> in order to identify the fraction responsible for inhibiting uterine smooth muscle contraction in pregnancy.<\/jats:p><\/jats:sec>Material and Methods<\/jats:title>Myometrial tissues were obtained from pregnant women and rats. The water\u2010 and lipid\u2010soluble fractions of shakuyaku\u2010kanzo\u2010to<\/jats:italic>, shakuyaku<\/jats:italic>, and kanzo<\/jats:italic> were obtained using the method of B<\/jats:styled-content>ligh and D<\/jats:styled-content>yer. Lipid\u2010soluble fractions were also partially purified using thin\u2010layer chromatography (TLC<\/jats:styled-content>) with a chloroform\u2009:\u2009methanol\u2009:\u2009water (65:25:4 by volume) solvent system to yield four TLC<\/jats:styled-content> fractions. The effect of each fraction on oxytocin\u2010induced myometrial contraction was examined in vitro<\/jats:italic>.<\/jats:p><\/jats:sec>Results<\/jats:title>Lipid\u2010soluble fractions obtained from shakuyaku\u2010kanzo\u2010to<\/jats:italic> and kanzo<\/jats:italic> inhibited myometrial contraction; water\u2010soluble fractions had no effect. Of the four TLC<\/jats:styled-content> fractions, the inhibitory effect was greatest with TLC<\/jats:styled-content> fraction 1 (0.75\u2009<\u2009R<\/jats:styled-content>f value\u2009\u2264\u20091.0). Neither the water\u2010soluble nor the lipid\u2010soluble fraction from shakuyaku<\/jats:italic> inhibited myometrial contraction.<\/jats:p><\/jats:sec>Conclusions<\/jats:title>These results suggest that lipid\u2010soluble substances with low polarity derived from kanzo<\/jats:italic> are responsible for the inhibitory effect of shakuyaku\u2010kanzo\u2010to<\/jats:italic> on myometrial contraction.<\/jats:p><\/jats:sec>","DOI":"10.1111\/jog.12618","type":"journal-article","created":{"date-parts":[[2014,11,25]],"date-time":"2014-11-25T08:22:21Z","timestamp":1416903741000},"page":"670-679","source":"Crossref","is-referenced-by-count":9,"title":["Lipid\u2010soluble fraction of S<\/scp>hakuyaku\u2010kanzo\u2010to<\/i> inhibits myometrial contraction in pregnant women"],"prefix":"10.1111","volume":"41","author":[{"given":"Genichiro","family":"Sumi","sequence":"first","affiliation":[{"name":"Department of Obstetrics and Gynecology Kansai Medical University Moriguchi Osaka Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Katsuhiko","family":"Yasuda","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynecology Kansai Medical University Moriguchi Osaka Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shoko","family":"Tsuji","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynecology Kansai Medical University Moriguchi Osaka Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chiharu","family":"Kanamori","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynecology Kansai Medical University Moriguchi Osaka Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tomoko","family":"Tsuzuki","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynecology Kansai Medical University Moriguchi Osaka 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